Polarization holographic microscope slide for birefringence imaging of anisotropic samples in microfluidics.

Birefringence is an important optical properties of anisotropic materials arising from the anisotropy of tissue microstructure. birefringence parameters have been found to be important to understand the architecture of many optical anisotropic material and polarization imaging has been applied in many fields of research in biology and medicine. Here, we propose a scheme for the miniaturize dual-channel polarization interferometer optical holograms to create holographic polarization microscope slide (P-HMS) suitable for integrating with (LoC) systems microfluidic lab-on-a-chip.

olympus confocal

Based on the P-HMS combined with simple reconstruction algorithms described in the paper, we can not only simultaneously realize holographic imaging of two orthogonal polarization components of dynamic samples in microfluidic channels but also a quantitative measurement of 2D information birefringence, both including birefringence the phase retardation and orientation optik- axis. Chip interferometer allows for off-axis polarization dual-channel digital holographic recording uses only a single light beam without any beam splitter or mirror. General quasi lane configuration and self-aligned design also makes it tolerant to vibration and misalignment. This work on the P-HMS can play a positive role in promoting the application of imaging birefringence in LOC microfluidic technology.


holographic microscope appeared as a suitable tool for in situ diagnostics and environmental monitoring, providing high-throughput, label-free, quantitative imaging capabilities through a small and compact device. In-line holographic microscope can be realized with contained costs, trade of complexity in decision phase and is limited to rare samples.

Here we present a 3D printed, cost effective and portable field off-axis holographic microscope slide hologram based microfluidic concepts. Our scheme eliminates the complexity of the reconstruction process, such as non-recurring and retrieval phase can be obtained by hologram demodulation. Our configuration ensures introduce flexibility in the definition of the optical scheme, exploited to realize a modular device with different features.

 Polarization holographic microscope slide for birefringence imaging of anisotropic samples in microfluidics.
Polarization holographic microscope slide for birefringence imaging of anisotropic samples in microfluidics.

A micro cell culture method utilizes modified microscope slides for use in fluorescent antibody and enzyme immunoassay.

A technique for the culture of a small number of mammalian cells on a microscope slide modified explained. The modified microscope slide is Bellco Glass, Inc., toxoplasmosis slides and cell culture used early part cow embryo lung cells and continuous cell lines origin pigs and dogs. Shear cell culture either infected or infected with a virus selected or obligate intracellular protozoanEncephalitozoon caniculi for use in the direct fluorescent antibody test and the indirect or peroxidase antiperoxidase immunosorbant test.

100-place microscope slide box (for 75 x 50 mm slides)

PO203 each
EUR 152.4

Box for 25 microscope slides purple colour

DD49719 EACH
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Vinyl microscope slides RINZL

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Microscope slide dispenser for box of 72 standard 25 x 75mm slides

M7100-SD 1/pack
EUR 83.89
Description: Microscope slide dispenser

Premium Plain Glass Microscope Slides, 144 /pk, 1 mm thickness, 25 mm wide by 73 mm long

M-ACC-GSL9 200 TIPS
EUR 65
Description: Premium Plain Glass Microscope Slides, 144 /pk, 1 mm thickness, 25 mm wide by 73 mm long

Microscope Slides Plain 1.0mm 76x52mm

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Superfrost® Plus microscope slides

PO103 box (72 slides)
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Proteinase-resistant microscope slides

PO102 100 slides
EUR 236.4

Microscope Slides Plain 1.2-1.5mm 76x26mm

MIC2154 PK50
EUR 4.08

Cardboard tray for 20 microscope slides

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Microscope slides, positive charged, 45° corners, frosted on one end, 25 x 75mm

M7100-45 72/pack
EUR 75.4
Description: Microscope slides positive charged

Microscope slides, positive charged, 90° corners, frosted on one end, 25 x 75mm

M7100-90 72/pack
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Description: Microscope slides positive charged

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DD37503 EACH
EUR 38.4

Plastic box for 100 microscope slides (blue colour)

DD37501 EACH
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Plastic box for 50 microscope slides (blue colour)

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Polystyrene box for 100 microscope slides blue colour

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Microscope Slide Boxes,25-Place, Red

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LC7009-010 10/PK
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Microscope Slide Boxes,25-Place, Red

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Microscope Slide Boxes,25-Place, Blue

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Microscope Slide Boxes,25-Place, Blue

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Microscope Slides 26X76mm Ground Edges Double Frosted 45° Angle -Silver-

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Microscope Slide Box True North

MIC4090 EACH
EUR 47.52

Microscope Slide Box 100 Place Tall

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Microscope Slide Polysine 75x25x1mm

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BRAND(TM) MICROSCOPE SLIDE CUT EDGES

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Microscope Slide Boxes,100-Place, Blue

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Microscope Slide Boxes,100-Place, Yellow

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Premium Microscope Slide Box 50-Place Cork Red

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Microscope Cover Slips No. 0 19mm dia

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Motic SMZ-168-BL Microscope

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BA310 Elite Trinocular microscope

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EUR 1809.56

Microscope Pack 1 CX23 100x Oil

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Microscope Pack 2 CX23 BIN LED

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Digi-Pad Microscope Tablet Camera

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Motic BA210 LED Microscope Binocular

MIC2004 EACH
EUR 1667.25

Motic BA310 LED Microscope Binocular

MIC2016 EACH
EUR 2250.85

ReadiUseâ„¢ microscope mounting solution

20009-50mL 50 mL
EUR 86
Description: The mounting medium is the solution in which the specimen is embedded, generally under a cover glass.

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VIKEM(R) VINYL MICROSCOPE COVER EXTRA

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MIGHTY SCOPE(TM) DIGITAL MICROSCOPE 5M

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ReadiUse™ microscope mounting solution

20009 50 mL
EUR 86

Motic SMZ-140-N2GG Stereo Microscope

MIC5900 EACH
EUR 774.22

Motic SMZ-143-N2GG Stereo Microscope

MIC5902 EACH
EUR 900.86

SMZ-171-Binocular P Stero Microscope

MIC5446 EACH
EUR 1552.36

Microscope Binoc MAGNUM-B planachrom obj

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SMZ-171-Binocular LED Stero Microscope

MIC5444 EACH
EUR 1989.73

SMZ-171-Trinocular P Stero Microscope

MIC5447 EACH
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BD BioCoat Fibronectin Coated 4 Well Tissue Culture Slides

354559 PK12
EUR 586.8

BD BioCoat Fibronectin Coated 8 Well Tissue Culture Slides

354631 PK12
EUR 514.8

Microscope Trinoc MAGNUM-T planachrom obj

MIC0107 EACH
EUR 2085.6

SMZ-171-Trinocular LED Stero Microscope

MIC5445 EACH
EUR 2108.54

BD BioCoat Collagen I Coated 4 Well Tissue Culture Slides

354557 PK12
EUR 573.6

BD BioCoat Collagen I Coated 8 Well Tissue Culture Slides

354630 PK12
EUR 465.6

Microscope Binoc MAGNUM-B pos phase contrast

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EUR 2624.4

SLS Lab Basics Star-24 LED Stereo Microscope

MIC2026 EACH
EUR 1422

Microscope Trinoc MAGNUM-T pos phase contrast

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EUR 2865.6

MiTeGen Kit V201 Stereo Microscope kit for MicroCrystallography:

ZV20-MTGKV201 1 KIT
EUR 16532
Description: MiTeGen Kit V201 Stereo Microscope kit for MicroCrystallography:- Ergonomic adjustable eyepieces- A wide array of accessories available- 7.5 to 150 x magnification range- ZEISS SteREO Discovery.V20- ZEISS SteREO Discovery.V20 body,- Human Interface Panel HIP,- Dust Protection Set M ,- Binoc Phototube Ergo Stereo 5-45 ,- Eyepiece PL 10x/23 Br foc ,- Folding Eyecup ,- Stand base Profile S,- Transmitted Light Equipment S- Cold-light source Zeiss CL6000 LED- 6200K color temperature,- control of intensity & 6 memory positions,- filter slider for 2 filters 35x26x4mm (sold separately)- Analyzer S Rotatable,- Polarizer D =84mm,- Coarse/fine drive w/column S 490mm- Mount S with 76 mm Diameter Support- Achromat S 1.0x Reo WD=63 lens

MiTeGen Kit V81 Stereo Microscope kit for Crystallography:

ZV20-MTGKV81 1 KIT
EUR 13998
Description: MiTeGen Kit V81 Stereo Microscope kit for Crystallography:- ZEISS Stereo Discovery V8 Microscope:- ZEISS Stereo Discovery.V8 body,- Dust Protection Set M ,- Binocular Phototube Ergo Stereo 5-45 ,- Eyepiece PL 10x/23 Br foc ,- Folding Eyecup ,- Stand base Profile S,- Transmitted Light Equipment S:- Cold-light source ZEISS CL6000 LED- 6200K color temperature, control of intensity & 6 memory positions,- filter slider for 2 filters (35 x 26 x 4mm, (filters sold separately)- Analyzer S Rotatable,- Polarizer D =84mm,- Manual focus drive fa/ Discovery,- Mount S with 76 mm Diameter Support- Achromatic S 1.0x Reo WD=63 lens

MiTeGen Kit V121 Stereo Microscope kit for Crystallography:

ZV20-MTGKV121 1 KIT
EUR 15282
Description: MiTeGen Kit V121 Stereo Microscope kit for Crystallography:- ZEISS Stereo Discovery V12 Microscope:- ZEISS Stereo Discovery.V8 body,- Dust Protection Set M ,- Binocular Phototube Ergo Stereo 5-45 ,- Eyepiece PL 10x/23 Br foc ,- Folding Eyecup ,- Stand base Profile S,- Transmitted Light Equipment S:- Cold-light source ZEISS CL6000 LED- 6200K color temperature,- control of intensity & 6 memory positions,- filter slider for 2 filters (35 x 26 x 4mm, (filters sold separately)- Analyzer S Rotatable,- Polarizer D =84mm,- Manual focus drive fa/ Discovery,- Mount S with 76 mm Diameter Support- Achromatic S 1.0x Reo WD=63 lens

MiTeGen Kit Stemi 305 Stereo Microscope kit for Crystallography:

ZV20-MTGK305 1 KIT
EUR 2589
Description: MiTeGen Kit Stemi 305 Stereo Microscope kit for Crystallography:Stemi 305 trinocular body w/ integrated camera portIncludes adapter for C-mount lensesStand K Edu w/ integrated transmissive LED cold light and polarizerSpot illuminator w/ polarizerAnalyzer (rotatable)

MiTeGen Crystallography Microscope Kit for Protein Crystallography:

ZV20-MTGK508-PC1 1 KIT
EUR 6492
Description: MiTeGen Crystallography Microscope Kit for Protein Crystallography:- Stemi 508 Stand M- Magnification: 10-80x (with 16x eyepieces)- Free Working Distance: 92 mm- Eyepieces (2 x 10x & 2 x 16x)- Cold light (LED) transillumination source with large working area stand- Rotatable and slidable mirror for brightfield, darkfield and oblique transillumination- Plain mirror side for crisp, diffuse mirror side for homogeneous illumination- Trinocular Camera Port Configuration- Camera Adapter 60N- Viewing angle 35 degrees with adjustable interocular distance- Camera Port- Analyzer (rotatable)- Transillumination Polarizer- Dual flex-neck top-side LED illumination

Max III Trinocular Compound Microscope with Achromatic Objectives

SLS2270 EACH
EUR 1194

Max III Trinocular Compound Microscope with Plan Achromatic Objectives

SLS2272 EACH
EUR 1525.2

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abx082013-100g 100 g
EUR 243.6

GELATIN

G07-102-10kg 10 kg
EUR 627.6

GELATIN

G07-102-2kg 2kg
EUR 183.6

GELATIN

G07-102-500g 500 g
EUR 93.6

Gelatin

G9764 100g
EUR 75.66

Steddy-T Trinocular Stereo Microscope with Diamond Base with Zoom Ratio 0.67x - 4.5x

SLS2106 EACH
EUR 1638

Magtex Trinocular Materials and Biological Microscope with Plan Achromatic Objectives

SLS2280 EACH
EUR 4160.4

Difco Gelatin

214340 EACH
EUR 116.4

Poly-L-Lysine Coated Slide

AR1065 50 slides/box
EUR 82.8

Gelatin Peptone

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NUTRIENT GELATIN

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NUTRIENT GELATIN

N14-108-500g 500 g
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0.1%Gelatin Solution

GLT-11301 100mL
EUR 49

Gelatin (4%), Acidulated

GSA125 125 ml
EUR 108

Gelatin (4%), Acidulated

GSA500 500 ml
EUR 171.6

Magnum Binocular Compound Microscope with Infinity Corrected Plan Achromatic Objectives with LED Illumination

MIC0122 EACH
EUR 2385.6

Magtex Trinocular Reflected and Transmitted Light Microscope with Polarisation and Plan Achromatic Objectives

SLS2282 EACH
EUR 6100.8

Recombinant Human Gelatin

7-05257 10mg Ask for price

Recombinant Human Gelatin

7-05258 50mg Ask for price

Recombinant Human Gelatin

7-05259 100mg Ask for price

Magnum Trinocular Compound Microscope with Infinity Corrected Plan Achromatic Objectives with LED Illumination

MIC0124 EACH
EUR 2496

C-Mount for All SLS Lab Basics Max III Microscopes

SLS2300 EACH
EUR 127.2

Gelatin Sepharose 4B 25mL

17095601 EACH
EUR 472.8

PANCREATIC DIGEST OF GELATIN

P16-100-10kg 10 kg
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PANCREATIC DIGEST OF GELATIN

P16-100-2kg 2kg
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PANCREATIC DIGEST OF GELATIN

P16-100-500g 500 g
EUR 105.6

Self-Quenched BODIPY Gelatin

7935-30 each
EUR 183.6

Recombinant (yeast) Human Gelatin

RP-921 5 mg
EUR 343.2

Bovine skin gelatin, type B

GELB11-N-10 10 g
EUR 123.6

Fish gelatin (cold water fish)

GELF14-N-50 50 ml
EUR 123.6

Porcine skin gelatin (type A)

GELP12-N-10 10 g
EUR 123.6

Gelatin 5% in Acetate Buffer

RRSP510-D 500ml
EUR 12.32

Gelatin 5% in Acetate Buffer

RRSP510-E 1L
EUR 18.46

Gelatin 5% in Acetate Buffer

RRSP510-F 2.5L
EUR 28.33

BRAND(TM) CAVITY SLIDES 76 X 26 MM 1.2

BR475505-50EA PK50
EUR 188.4

Sartorius Gelatin Membrane Holder

FIL3154 EACH
EUR 145.2

Gelatin, Fluorescein (FITC) Conjugate

M1303-1 each
EUR 117.6

Gelatin, Fluorescein (FITC) Conjugate

M1303-5 each
EUR 261.6

C-Mount for All SLS Lab Basics Magnum and Magtex Microscopes

SLS2302 EACH
EUR 145.2

CORNING® COLLAGEN COATED MICROCARRIERS, 1 VIAL, 10G

3786 10g/pk
EUR 198
Description: Microcarriers; Corning Microcarriers

Slide Box Red 25 Slides

MIC3566 EACH
EUR 22.8

Gelatin, powdered, from porcine skin

GE2739-100G 100 g
EUR 55.2

Gelatin, powdered, from porcine skin

GE2739-1KG 1 kg
EUR 156

Gelatin, powdered, from porcine skin

GE2739-250G 250 g
EUR 74.4

Gelatin, powdered, from porcine skin

GE2739-500G 500 g
EUR 103.2

Slide Box Blue 25 Slides

MIC3562 EACH
EUR 23.75

Slide Box Green 25 Slides

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EUR 23.75

Slide Box White 25 Slides

MIC3570 EACH
EUR 23.75

Rabbit Anti-Fish gelatin antiserum

GELF14-S 100 ul
EUR 548.4

Gelatinase (Gelatin Degradation/Zymography) Assay Kit (Fluorometric)

K444-100 each
EUR 516

Rabbit Anti-Bovine gelatin antiserum

GELB11-S 100 ul
EUR 548.4

We discuss the trade-offs and practical design rules to follow to develop a microscope DH by the proposed solution. Using our prototype, we picture flowing marine microalgae, beads of polystyrene, E.coli and microplastics. We detail the effects on the performance and cost of each of the parameters, design and hardware selection, guiding the reader toward the realization of optimized device that can be used out of the lab by non-expert users for point of care testing.