Polarization holographic microscope slide for birefringence imaging of anisotropic samples in microfluidics.

Birefringence is an important optical properties of anisotropic materials arising from the anisotropy of tissue microstructure. birefringence parameters have been found to be important to understand the architecture of many optical anisotropic material and polarization imaging has been applied in many fields of research in biology and medicine. Here, we propose a scheme for the miniaturize dual-channel polarization interferometer optical holograms to create holographic polarization microscope slide (P-HMS) suitable for integrating with (LoC) systems microfluidic lab-on-a-chip.

olympus confocal

Based on the P-HMS combined with simple reconstruction algorithms described in the paper, we can not only simultaneously realize holographic imaging of two orthogonal polarization components of dynamic samples in microfluidic channels but also a quantitative measurement of 2D information birefringence, both including birefringence the phase retardation and orientation optik- axis. Chip interferometer allows for off-axis polarization dual-channel digital holographic recording uses only a single light beam without any beam splitter or mirror. General quasi lane configuration and self-aligned design also makes it tolerant to vibration and misalignment. This work on the P-HMS can play a positive role in promoting the application of imaging birefringence in LOC microfluidic technology.


holographic microscope appeared as a suitable tool for in situ diagnostics and environmental monitoring, providing high-throughput, label-free, quantitative imaging capabilities through a small and compact device. In-line holographic microscope can be realized with contained costs, trade of complexity in decision phase and is limited to rare samples.

Here we present a 3D printed, cost effective and portable field off-axis holographic microscope slide hologram based microfluidic concepts. Our scheme eliminates the complexity of the reconstruction process, such as non-recurring and retrieval phase can be obtained by hologram demodulation. Our configuration ensures introduce flexibility in the definition of the optical scheme, exploited to realize a modular device with different features.

 Polarization holographic microscope slide for birefringence imaging of anisotropic samples in microfluidics.
Polarization holographic microscope slide for birefringence imaging of anisotropic samples in microfluidics.

A micro cell culture method utilizes modified microscope slides for use in fluorescent antibody and enzyme immunoassay.

A technique for the culture of a small number of mammalian cells on a microscope slide modified explained. The modified microscope slide is Bellco Glass, Inc., toxoplasmosis slides and cell culture used early part cow embryo lung cells and continuous cell lines origin pigs and dogs. Shear cell culture either infected or infected with a virus selected or obligate intracellular protozoanEncephalitozoon caniculi for use in the direct fluorescent antibody test and the indirect or peroxidase antiperoxidase immunosorbant test.

100-place microscope slide box (for 75 x 50 mm slides)

PO203 each
EUR 152.4

FUNNEL, 75 MM, PP

6120P-75 12/pk
EUR 48
Description: Reusable Plastics; Reusable Funnels

Box for 25 microscope slides purple colour

DD49719 EACH
EUR 15.28

Box for 25 microscope slides orange colour

DD49720 EACH
EUR 15.28

Box for 25 microscope slides turquoise colour

DD49721 EACH
EUR 15.28

Premium Plain Glass Microscope Slides, 144 /pk, 1 mm thickness, 25 mm wide by 73 mm long

M-ACC-GSL9 200 TIPS
EUR 65
Description: Premium Plain Glass Microscope Slides, 144 /pk, 1 mm thickness, 25 mm wide by 73 mm long

Microscope slide dispenser for box of 72 standard 25 x 75mm slides

M7100-SD 1/pack
EUR 83.89
Description: Microscope slide dispenser

Proteinase-resistant microscope slides

PO102 100 slides
EUR 236.4

Vinyl microscope slides RINZL

DD52951 PK144
EUR 24.62

Microscope slides, positive charged, 45° corners, frosted on one end, 25 x 75mm

M7100-45 72/pack
EUR 75.4
Description: Microscope slides positive charged

Microscope slides, positive charged, 90° corners, frosted on one end, 25 x 75mm

M7100-90 72/pack
EUR 75.4
Description: Microscope slides positive charged

Superfrost® Plus microscope slides

PO103 box (72 slides)
EUR 123.6

12 X 75 MM POLYPROPYLENE CULTURE TUBE

T-1275-PP 500/pk
EUR 92.4
Description: Micro Tubes; Screw Cap Macro Tubes - Axygen

12 X 75 MM POLYSTYRENE CULTURE TUBE.

T-1275-PS 500/pk
EUR 93.6
Description: Micro Tubes; Screw Cap Macro Tubes - Axygen

Terumo Agani needle G 25 0.5 x 25 mm

DD050139B PK100
EUR 5.93

Microscope Slides Plain 1.2-1.5mm 76x26mm

MIC2154 PK50
EUR 3.88

Microscope Slides Plain 1.0-1.2mm 76x52mm

MIC2156 PK50
EUR 15.73

Cardboard tray for 20 microscope slides

DD65205 EACH
EUR 15.85

Axygen Culture Tube 12 x 75 mm Polypropylene

AXY2174 PK1000
EUR 75.24

BRAND(TM) CAVITY SLIDES 76 X 26 MM 1.2

BR475505-50EA PK50
EUR 149.91

Oligo (dT)25 Coated Gold Particles

OdT(25)AuP-002-1 1 mL
EUR 462
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Oligo (dT)25 Coated Gold Particles

OdT(25)AuP-002-5 5 mL
EUR 1254
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Mini gel tubes, 7 x 2.5 x 75 mm, 8/pk

2394122 6unit
EUR 315.6

Anti-Human IgM Antibody | MM-80A

MM-80A 1.0 mg
EUR 334
Description:

Anti-Human IgM Antibody | MM-80A | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Secondary Antibody

Antibody Clonality: Monoclonal

Dual-Thickness MicroMounts, box of 20 with 75 micron aperture mounts; mounted on 18 mm rods

M2-L18SP-75 20 MOUNTS
EUR 112
Description: Dual-Thickness MicroMounts, box of 20 with 75 micron aperture mounts; mounted on 18 mm rods

DiagNano Fluorophore Labeled Gold Nanoparticles, 75 nm

GFL-75 1 mL
EUR 1263.6

MgCl2 25 mM

BA00154 5x1.8ml
EUR 74.4
Description: High purity buffer for various PCR applications.

Single channel mini-pipette (130 mm) 25 ul, autoclavable

SCMP-25 1
EUR 110.4

Polystyrene box for 100 microscope slides blue colour

DD139537 EACH
EUR 10.49

Polystyrene box for 100 microscope slides green colour

DD139538 EACH
EUR 10.49

Plastic box for 100 microscope slides (blue colour)

DD37501 EACH
EUR 29.64

Plastic box for 100 microscope slides (red colour)

DD37503 EACH
EUR 29.64

Plastic box for 50 microscope slides (blue colour)

DD37507 EACH
EUR 25.08

Microscope Slide Boxes,25-Place, Blue

LC7007-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Blue

LC7007-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Blue

LC7007-100 100/CS
EUR 684

Microscope Slide Boxes,25-Place, Green

LC7008-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Green

LC7008-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Green

LC7008-100 100/CS
EUR 684

Microscope Slide Boxes,25-Place, Red

LC7009-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Red

LC7009-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Red

LC7009-100 100/CS
EUR 684

Microscope Slide Boxes,25-Place, Yellow

LC7010-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Yellow

LC7010-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Yellow

LC7010-100 100/CS
EUR 684

Microscope Slide Boxes,25-Place, White

LC7011-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, White

LC7011-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, White

LC7011-100 100/CS
EUR 684

Microscope Slide Box 25 ULT safe

MIC4092 EACH
EUR 17.62

Star-Frost slides 76 x 26 mm (standard version) colour : orange

DD100205O PK50
EUR 12.54

Regular Unleaded Gasoline 75% Weathered

S-WG-75 1ML
EUR 221.16

COVER GLASS, NO. 2, SQUARE 25 X 25MM

2855-25 50/pk
EUR 103.2
Description: Cover Glass; Cover Glass, CGW

COVER GLASS, NO. 1, SQUARE 25 X 25MM

2845-25 100/pk
EUR 134.4
Description: Cover Glass; Cover Glass, CGW

Weighing cup shock-resistant white colour 140 x 140 x 25 mm

DD45107 PK500
EUR 136.8

Amyloid (Control Slides)

TCS0003-25 25 Slides
EUR 278.4

Argentaffin (Control Slides)

TCS0004-25 25 Slides
EUR 278.4

Fungus (Control Slides)

TCS0005-25 25 Slides
EUR 278.4

Bielschowsky's (Control Slides)

TCS0006-25 25 Slides
EUR 278.4

Calcium (Control Slides)

TCS0007-25 25 Slides
EUR 278.4

Elastic (Control Slides)

TCS0008-25 25 Slides
EUR 278.4

Melanin (Control Slides)

TCS0015-25 25 Slides
EUR 278.4

Mucin (Control Slides)

TCS0016-25 25 Slides
EUR 278.4

Reticulum (Control Slides)

TCS0019-25 25 Slides
EUR 278.4

Slide Box Blue 25 Slides

MIC3562 EACH
EUR 19.13

Slide Box Green 25 Slides

MIC3564 EACH
EUR 19.13

Slide Box Red 25 Slides

MIC3566 EACH
EUR 19.13

Slide Box White 25 Slides

MIC3570 EACH
EUR 19.13

dNTPs (25 mM each)

RK20101 100 μL Ask for price

Gelatin

abx082013-100g 100 g
EUR 243.6

GELATIN

G07-102-10kg 10 kg
EUR 627.6

GELATIN

G07-102-2kg 2kg
EUR 183.6

GELATIN

G07-102-500g 500 g
EUR 93.6

Gelatin

G9764 100g
EUR 75.66

DB 5.7 Single block for 8 x 25 mm vials

BLO6072 EACH
EUR 164.16

Divider for 25 tubes (for boxes 136 x 136 mm)

DD39528 EACH
EUR 3.65

MM 11253

B7505-25 25 mg
EUR 603.6

MM-102

B2364-25
EUR 705.6

2947 75 X 25MM

2947-75X25 0,5/pk
EUR 297.6
Description: Slides; Slides

2947 75 X 38MM

2947-75X38 0,5/pk
EUR 250.8
Description: Slides; Slides

2947 75 X 50MM

2947-75X50 0,5/pk
EUR 318
Description: Slides; Slides

2948 75 X 25MM

2948-75X25 0,5/pk
EUR 380.4
Description: Slides; Slides

2949 75 X 25MM

2949-75X25 0,5/pk
EUR 486
Description: Slides; Slides

Alcian Blue (Control Slides)

TCS0002-25 25 Slides
EUR 278.4

Helicobacter Pylori (Control Slides)

TCS0011-25 25 Slides
EUR 278.4

Iron - Liver (Control Slides)

TCS0013-25 25 Slides
EUR 278.4

Mast Cell (Control Slides)

TCS0014-25 25 Slides
EUR 278.4

PAS/Glycogen (Control Slides)

TCS0017-25 25 Slides
EUR 278.4

Trichrome - Colon (Control Slides)

TCS0021-25 25 Slides
EUR 278.4

Trichrome - Liver (Control Slides)

TCS0022-25 25 Slides
EUR 278.4

Anti-Bovine IgM Antibody | MM-10A-2B10

MM-10A-2B10 1.0 mg
EUR 516
Description:

Anti-Bovine IgM Antibody | MM-10A-2B10 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Secondary Antibody

Antibody Clonality: Monoclonal

Anti-Cat IgM Antibody | MM-20A-1C8

MM-20A-1C8 1.0 mg
EUR 448
Description:

Anti-Cat IgM Antibody | MM-20A-1C8 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Secondary Antibody

Antibody Clonality: Monoclonal

Anti-Sheep IgM Antibody | MM-35A-2G3

MM-35A-2G3 1.0 mg
EUR 516
Description:

Anti-Sheep IgM Antibody | MM-35A-2G3 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Secondary Antibody

Antibody Clonality: Monoclonal

Anti-Dog IgM Antibody | MM-40A-3H6

MM-40A-3H6 1.0 mg
EUR 448
Description:

Anti-Dog IgM Antibody | MM-40A-3H6 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Secondary Antibody

Antibody Clonality: Monoclonal

Anti-Human IgM Antibody | MM-80A-1A5

MM-80A-1A5 1.0 mg
EUR 334
Description:

Anti-Human IgM Antibody | MM-80A-1A5 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Secondary Antibody

Antibody Clonality: Monoclonal

Anti-Human IgM Antibody | MM-80A-1D6

MM-80A-1D6 1.0 mg
EUR 334
Description:

Anti-Human IgM Antibody | MM-80A-1D6 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Secondary Antibody

Antibody Clonality: Monoclonal

Anti-Human IgM Antibody | MM-80A-1H12

MM-80A-1H12 1.0 mg
EUR 334
Description:

Anti-Human IgM Antibody | MM-80A-1H12 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Secondary Antibody

Antibody Clonality: Monoclonal

Anti-Human IgM Antibody | MM-80A-208

MM-80A-208 1.0 mg
EUR 334
Description:

Anti-Human IgM Antibody | MM-80A-208 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Secondary Antibody

Antibody Clonality: Monoclonal

COVER GLASS, NO. 1 1/2, SQUARE 25 X 25MM

2850-25 100/pk
EUR 272.4
Description: Cover Glass; Cover Glass, CGW

Sulfur 75 ug/g (75 PPM) for AA and ICP 100 grams in no.2 Diesel Fuel

SDFS-75-Y 100ML
EUR 102.6

Grain Sieve 3.55 mm x 20 mm

SIE3080 EACH
EUR 210.9

Grain Sieve 2.24 mm x 20 mm

SIE3084 EACH
EUR 210.9

Grain Sieve 2.20 mm x 20 mm

SIE3086 EACH
EUR 210.9

Grain Sieve 2.00 mm x 20 mm

SIE3088 EACH
EUR 210.9

Grain Sieve 1.80 mm x 20 mm

SIE3092 EACH
EUR 210.9

Grain Sieve 1.70 mm x 20 mm

SIE3094 EACH
EUR 210.9

Grain Sieve 1.00 mm x 20 mm

SIE3096 EACH
EUR 210.9

Slide Box White for 25 Slides

MIC4000 EACH
EUR 9.69

PTFE pipe o int. x o ext. = 7 x 8.5 mm length 25 m

DD91947 EACH
EUR 414.96

MgCl2 25 mM 1.8 ml

BA00153 1.8ml
EUR 66
Description: High purity buffer for various PCR applications.

Spreader stainless steel 25 mm

Z363375-1EA EACH
EUR 21.89

Plastic box for 100 microscope slides (assorted colours) cork bottom

DD10197 PK5
EUR 83.22

Microscope Slides 26X76mm Ground Edges Double Frosted 45° Angle -Silver-

VBS656 PK2500
EUR 232.56

Oligo (dT)25 Coated Polystyrene Beads

OdT25P-100-4 4 mL
EUR 493.2
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

NUTRIENT GELATIN

N14-108-10kg 10 kg
EUR 1088.4

NUTRIENT GELATIN

N14-108-2kg 2kg
EUR 283.2

NUTRIENT GELATIN

N14-108-500g 500 g
EUR 121.2

Difco Gelatin

214340 EACH
EUR 101.46

Gelatin Peptone

MED1504 EACH
EUR 78.66

Corning 2947 75 X 25mm

294775X25 PK1440
EUR 361.38

Adapter 17.5 x 75-100mm

E5920708007 EACH
EUR 281.58

1.1 MM X 4 MM GEL CUTTING TIPS

TGL-1140 250/pk
EUR 357.6
Description: Non Filter Tips; Pipette Tips - Axygen

1.1 MM X 4 MM GEL CUTTING TIPS

TGL-1140-R 480/pk
EUR 488.4
Description: Non Filter Tips; Pipette Tips - Axygen

1.1 MM X 6.5 MM GEL CUTTING TIPS

TGL-1165 250/pk
EUR 357.6
Description: Non Filter Tips; Pipette Tips - Axygen

1.1 MM X 6.5 MM GEL CUTTING TIPS

TGL-1165-R 480/pk
EUR 488.4
Description: Non Filter Tips; Pipette Tips - Axygen

Slide Tray Singles x 20 Slides

MIC4200 EACH
EUR 14.61

Cardboard freezer box, hinged lid, insert for 25 snap-cap 5ml tubes, 5.25 x 5.25 x 3 inches

C2580-25 5/pack
EUR 76.04

Rod for base M10 zinc-coated steel 18/10 diameter 12 x 1500 mm

DD076602 EA
EUR 24.05

BD BioCoat Fibronectin Coated 4 Well Tissue Culture Slides

354559 PK12
EUR 557.46

BD BioCoat Fibronectin Coated 8 Well Tissue Culture Slides

354631 PK12
EUR 477.66

BRAND(TM) STAINING TRAY FOR 25 SLIDES

BR471400-5EA PK5
EUR 46.63

PAS/Glycogen, For Digestion (Control Slides)

TCS0024-25 25 Slides
EUR 278.4

Core Panel Multi-Tumor control slides

TS900-25 Set of 25
EUR 582

Breast Panel Multi-Tumor control slides

TS901-25 Set of 25
EUR 582

CD Marker Multi-Tumor control slides

TS902-25 Set of 25
EUR 582

Interseal Sheets148 x 98 mm

PCR0560 PK100
EUR 189.24

mt comb 25 sample, 1.0 mm

EHS3300-CMT25-1.0 ea
EUR 78

mt comb 25 sample, 1.5 mm

EHS3300-CMT25-1.5 ea
EUR 78

mt comb 25 sample, 1.0 mm

EHS3600-CMT25-1.0 ea
EUR 87.6

mt comb 25 sample, 1.5 mm

EHS3600-CMT25-1.5 ea
EUR 87.6

comb 1 mm thick 25 sample

EHS1200-C25-1.0 ea
EUR 49.2

comb 1.5 mm thick 25 sample

EHS1200-C25-1.5 ea
EUR 49.2

comb 1 mm thick 25 sample

EHS1400-C25-1.0 ea
EUR 67.2

comb 1.5 mm thick 25 sample

EHS1400-C25-1.5 ea
EUR 67.2

1L DMEM glucose 25 mM HEPES

15-018-CM PK6
EUR 304.38

500mL DMEM glucose 25 mM HEPES

15-018-CV PK6
EUR 181.26

25 mm Test Tube Foam Rack

MIX1116 EACH
EUR 42.18

Black box 130 x 130 x 50 mm

DD139735 EACH
EUR 14.82

Axygen Gel Cutting Tips 1.1 mm x 6.5 mm

AXY2216 PK2500
EUR 613.32

Adapter 16 x 75-100mm highcap

E5920720007 EACH
EUR 281.58

BD BioCoat Collagen I Coated 4 Well Tissue Culture Slides

354557 PK12
EUR 528.96

BD BioCoat Collagen I Coated 8 Well Tissue Culture Slides

354630 PK12
EUR 430.92

Star-Frost slides 76x26 mm standard version white colour

DD100205 PK50
EUR 12.54

18 mm Circular Glass Cover Slides - Plain, 1000/CS

M-CSL18-103 1000 pcs.
EUR 218
Description: 18 mm Circular Glass Cover Slides - Plain, 1000/CS

18 mm Circular Glass Cover Slides - Siliconized, 500/CS

M-CSL18-105S 500 pcs.
EUR 221
Description: 18 mm Circular Glass Cover Slides - Siliconized, 500/CS

18 mm Square Glass Cover Slides - Superhydrophobic, 500/CS

M-CSL18-110 1 Slides
EUR 246
Description: 18 mm Square Glass Cover Slides - Superhydrophobic, 500/CS

22 mm Circular Glass Cover Slides - Plain, 1000/CS

M-CSL22-104 1000 pcs.
EUR 270
Description: 22 mm Circular Glass Cover Slides - Plain, 1000/CS

22 mm Circular Glass Cover Slides - Siliconized, 500/CS

M-CSL22-106S 500 pcs.
EUR 244
Description: 22 mm Circular Glass Cover Slides - Siliconized, 500/CS

Reservoir 1 well 100 mL (width 75 mm)

DD47179 PK10
EUR 33.86

Reservoir 8 wells 10 mL (width 75 mm)

DD47180 PK10
EUR 33.86

Recombinant Human Gelatin

7-05257 10mg Ask for price

Recombinant Human Gelatin

7-05258 50mg Ask for price

We discuss the trade-offs and practical design rules to follow to develop a microscope DH by the proposed solution. Using our prototype, we picture flowing marine microalgae, beads of polystyrene, E.coli and microplastics. We detail the effects on the performance and cost of each of the parameters, design and hardware selection, guiding the reader toward the realization of optimized device that can be used out of the lab by non-expert users for point of care testing.