Birefringence is an important optical properties of anisotropic materials arising from the anisotropy of tissue microstructure. birefringence parameters have been found to be important to understand the architecture of many optical anisotropic material and polarization imaging has been applied in many fields of research in biology and medicine. Here, we propose a scheme for the miniaturize dual-channel polarization interferometer optical holograms to create holographic polarization microscope slide (P-HMS) suitable for integrating with (LoC) systems microfluidic lab-on-a-chip.

Based on the P-HMS combined with simple reconstruction algorithms described in the paper, we can not only simultaneously realize holographic imaging of two orthogonal polarization components of dynamic samples in microfluidic channels but also a quantitative measurement of 2D information birefringence, both including birefringence the phase retardation and orientation optik- axis. Chip interferometer allows for off-axis polarization dual-channel digital holographic recording uses only a single light beam without any beam splitter or mirror. General quasi lane configuration and self-aligned design also makes it tolerant to vibration and misalignment. This work on the P-HMS can play a positive role in promoting the application of imaging birefringence in LOC microfluidic technology.

holographic microscope appeared as a suitable tool for in situ diagnostics and environmental monitoring, providing high-throughput, label-free, quantitative imaging capabilities through a small and compact device. In-line holographic microscope can be realized with contained costs, trade of complexity in decision phase and is limited to rare samples.

Here we present a 3D printed, cost effective and portable field off-axis holographic microscope slide hologram based microfluidic concepts. Our scheme eliminates the complexity of the reconstruction process, such as non-recurring and retrieval phase can be obtained by hologram demodulation. Our configuration ensures introduce flexibility in the definition of the optical scheme, exploited to realize a modular device with different features.

A micro cell culture method utilizes modified microscope slides for use in fluorescent antibody and enzyme immunoassay.

A technique for the culture of a small number of mammalian cells on a microscope slide modified explained. The modified microscope slide is Bellco Glass, Inc., toxoplasmosis slides and cell culture used early part cow embryo lung cells and continuous cell lines origin pigs and dogs. Shear cell culture either infected or infected with a virus selected or obligate intracellular protozoanEncephalitozoon caniculi for use in the direct fluorescent antibody test and the indirect or peroxidase antiperoxidase immunosorbant test.

25-place microscope slide box (for 75 x 25 mm slides)
PO202	FD Neurotechnologies	each	EUR 59.1
Description: Best quality products

100-place microscope slide box (for 75 x 25 mm slides)
PO201	FD Neurotechnologies	each	EUR 67.03
Description: Best quality products

100-place microscope slide box (for 75 x 50 mm slides)
PO203	FD Neurotechnologies	each	EUR 114.36
Description: Best quality products

FUNNEL, 75 MM, PP
6120P-75	CORNING	12/pk	EUR 40
Description: Reusable Plastics; Reusable Funnels

Microscope slide dispenser for box of 72 standard 25 x 75mm slides
M7100-SD	MTC Bio	1/pack	EUR 69.91
Description: Microscope slide dispenser

Proteinase-resistant microscope slides
PO102	FD Neurotechnologies	100 slides	EUR 186.41
Description: Best quality products

Microscope slides, positive charged, 45° corners, frosted on one end, 25 x 75mm
M7100-45	MTC Bio	72/pack	EUR 62.83
Description: Microscope slides positive charged

Microscope slides, positive charged, 90° corners, frosted on one end, 25 x 75mm
M7100-90	MTC Bio	72/pack	EUR 62.83
Description: Microscope slides positive charged

Superfrost® Plus microscope slides
PO103	FD Neurotechnologies	box (72 slides)	EUR 94.55
Description: Best quality products

12 X 75 MM POLYPROPYLENE CULTURE TUBE
T-1275-PP	CORNING	500/pk	EUR 77
Description: Micro Tubes; Screw Cap Macro Tubes - Axygen

12 X 75 MM POLYSTYRENE CULTURE TUBE.
T-1275-PS	CORNING	500/pk	EUR 78
Description: Micro Tubes; Screw Cap Macro Tubes - Axygen

Mini gel tubes, 7 x 2.5 x 75 mm, 8/pk
2394122	Atto	6unit	EUR 263

Oligo (dT)25 Coated Gold Particles
OdT(25)AuP-002-1	Spherotech	1 mL	EUR 385
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Oligo (dT)25 Coated Gold Particles
OdT(25)AuP-002-5	Spherotech	5 mL	EUR 1045
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

DiagNano Fluorophore Labeled Gold Nanoparticles, 75 nm
GFL-75	Creative Diagnostics	1 mL	EUR 1053

MgCl2 25 mM
BA00154	Bioatlas	5x1.8ml	EUR 62
Description: High purity buffer for various PCR applications.

Microscope Slide Boxes,25-Place, Blue
LC7007-001	GenDepot	EA	EUR 66

Microscope Slide Boxes,25-Place, Blue
LC7007-010	GenDepot	10/PK	EUR 124

Microscope Slide Boxes,25-Place, Blue
LC7007-100	GenDepot	100/CS	EUR 570

Microscope Slide Boxes,25-Place, Green
LC7008-001	GenDepot	EA	EUR 66

Microscope Slide Boxes,25-Place, Green
LC7008-010	GenDepot	10/PK	EUR 124

Microscope Slide Boxes,25-Place, Green
LC7008-100	GenDepot	100/CS	EUR 570

Microscope Slide Boxes,25-Place, Red
LC7009-001	GenDepot	EA	EUR 66

Microscope Slide Boxes,25-Place, Red
LC7009-010	GenDepot	10/PK	EUR 124

Microscope Slide Boxes,25-Place, Red
LC7009-100	GenDepot	100/CS	EUR 570

Microscope Slide Boxes,25-Place, Yellow
LC7010-001	GenDepot	EA	EUR 66

Microscope Slide Boxes,25-Place, Yellow
LC7010-010	GenDepot	10/PK	EUR 124

Microscope Slide Boxes,25-Place, Yellow
LC7010-100	GenDepot	100/CS	EUR 570

Microscope Slide Boxes,25-Place, White
LC7011-001	GenDepot	EA	EUR 66

Microscope Slide Boxes,25-Place, White
LC7011-010	GenDepot	10/PK	EUR 124

Microscope Slide Boxes,25-Place, White
LC7011-100	GenDepot	100/CS	EUR 570

Single channel mini-pipette (130 mm) 25 ul, autoclavable
SCMP-25	Alpha Diagnostics	1	EUR 92

COVER GLASS, NO. 2, SQUARE 25 X 25MM
2855-25	CORNING	50/pk	EUR 86
Description: Cover Glass; Cover Glass, CGW

COVER GLASS, NO. 1, SQUARE 25 X 25MM
2845-25	CORNING	100/pk	EUR 112
Description: Cover Glass; Cover Glass, CGW

Amyloid (Control Slides)
TCS0003-25	ScyTek Laboratories	25 Slides	EUR 232

Argentaffin (Control Slides)
TCS0004-25	ScyTek Laboratories	25 Slides	EUR 232

Fungus (Control Slides)
TCS0005-25	ScyTek Laboratories	25 Slides	EUR 232

Bielschowsky's (Control Slides)
TCS0006-25	ScyTek Laboratories	25 Slides	EUR 232

Calcium (Control Slides)
TCS0007-25	ScyTek Laboratories	25 Slides	EUR 232

Elastic (Control Slides)
TCS0008-25	ScyTek Laboratories	25 Slides	EUR 232

Melanin (Control Slides)
TCS0015-25	ScyTek Laboratories	25 Slides	EUR 232

Mucin (Control Slides)
TCS0016-25	ScyTek Laboratories	25 Slides	EUR 232

Reticulum (Control Slides)
TCS0019-25	ScyTek Laboratories	25 Slides	EUR 232

2947 75 X 25MM
2947-75X25	CORNING	0,5/pk	EUR 248
Description: Slides; Slides

2947 75 X 38MM
2947-75X38	CORNING	0,5/pk	EUR 209
Description: Slides; Slides

2947 75 X 50MM
2947-75X50	CORNING	0,5/pk	EUR 265
Description: Slides; Slides

2948 75 X 25MM
2948-75X25	CORNING	0,5/pk	EUR 317
Description: Slides; Slides

2949 75 X 25MM
2949-75X25	CORNING	0,5/pk	EUR 405
Description: Slides; Slides

Gelatin
abx082013-100g	Abbexa	100 g	EUR 203
Shipped within 5-10 working days.

GELATIN
G07-102-10kg	Alphabiosciences	10 kg	EUR 523

GELATIN
G07-102-2kg	Alphabiosciences	2kg	EUR 153

GELATIN
G07-102-500g	Alphabiosciences	500 g	EUR 78

Gelatin
G9764	Bio Basic	100g	EUR 63.05
Product category: Culture Media/Other

Oligo (dT)25 Coated Polystyrene Beads
OdT25P-100-4	Spherotech	4 mL	EUR 411
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Alcian Blue (Control Slides)
TCS0002-25	ScyTek Laboratories	25 Slides	EUR 232

Helicobacter Pylori (Control Slides)
TCS0011-25	ScyTek Laboratories	25 Slides	EUR 232

Iron - Liver (Control Slides)
TCS0013-25	ScyTek Laboratories	25 Slides	EUR 232

Mast Cell (Control Slides)
TCS0014-25	ScyTek Laboratories	25 Slides	EUR 232

PAS/Glycogen (Control Slides)
TCS0017-25	ScyTek Laboratories	25 Slides	EUR 232

Trichrome - Colon (Control Slides)
TCS0021-25	ScyTek Laboratories	25 Slides	EUR 232

Trichrome - Liver (Control Slides)
TCS0022-25	ScyTek Laboratories	25 Slides	EUR 232

MM-102
B2364-25	Biovision		EUR 588

MM 11253
B7505-25	ApexBio	25 mg	EUR 503

COVER GLASS, NO. 1 1/2, SQUARE 25 X 25MM
2850-25	CORNING	100/pk	EUR 227
Description: Cover Glass; Cover Glass, CGW

MgCl2 25 mM 1.8 ml
BA00153	Bioatlas	1.8ml	EUR 55
Description: High purity buffer for various PCR applications.

1.1 MM X 4 MM GEL CUTTING TIPS
TGL-1140	CORNING	250/pk	EUR 298
Description: Non Filter Tips; Pipette Tips - Axygen

1.1 MM X 4 MM GEL CUTTING TIPS
TGL-1140-R	CORNING	480/pk	EUR 407
Description: Non Filter Tips; Pipette Tips - Axygen

1.1 MM X 6.5 MM GEL CUTTING TIPS
TGL-1165	CORNING	250/pk	EUR 298
Description: Non Filter Tips; Pipette Tips - Axygen

1.1 MM X 6.5 MM GEL CUTTING TIPS
TGL-1165-R	CORNING	480/pk	EUR 407
Description: Non Filter Tips; Pipette Tips - Axygen

100 mM Triton X-100
603	AthenaES	100 ml	EUR 98

Cardboard freezer box, hinged lid, insert for 25 snap-cap 5ml tubes, 5.25 x 5.25 x 3 inches
C2580-25	MTC Bio	5/pack	EUR 63.37

Fish Gelatin
22011	Biotium	100G	EUR 106
Description: Minimum order quantity: 1 unit of 100G

NUTRIENT GELATIN
N14-108-10kg	Alphabiosciences	10 kg	EUR 907

NUTRIENT GELATIN
N14-108-2kg	Alphabiosciences	2kg	EUR 236

NUTRIENT GELATIN
N14-108-500g	Alphabiosciences	500 g	EUR 101

HAV antibody IgM ELISA test
75	Biobase	96T/Box	Ask for price
Area of application: Hepatitis testing
Description: ELISA based test for quantitative detection of HAV antibody IgM

mt comb 25 sample, 1.0 mm
EHS3600-CMT25-1.0	Consort	ea	EUR 73

mt comb 25 sample, 1.5 mm
EHS3600-CMT25-1.5	Consort	ea	EUR 73

comb 1 mm thick 25 sample
EHS1200-C25-1.0	Consort	ea	EUR 41

comb 1.5 mm thick 25 sample
EHS1200-C25-1.5	Consort	ea	EUR 41

comb 1 mm thick 25 sample
EHS1400-C25-1.0	Consort	ea	EUR 56

comb 1.5 mm thick 25 sample
EHS1400-C25-1.5	Consort	ea	EUR 56

mt comb 25 sample, 1.0 mm
EHS3300-CMT25-1.0	Consort	ea	EUR 65

mt comb 25 sample, 1.5 mm
EHS3300-CMT25-1.5	Consort	ea	EUR 65

PAS/Glycogen, For Digestion (Control Slides)
TCS0024-25	ScyTek Laboratories	25 Slides	EUR 232

Core Panel Multi-Tumor control slides
TS900-25	Innovex	Set of 25	EUR 485

Breast Panel Multi-Tumor control slides
TS901-25	Innovex	Set of 25	EUR 485

CD Marker Multi-Tumor control slides
TS902-25	Innovex	Set of 25	EUR 485

AXYGEN® 25-WELL COMB FOR USE WITH 10 CM GEL BOX, 1.0 MM THICKNESS
HGB10-25-1	CORNING	1/pk	EUR 59
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 25-WELL COMB FOR USE WITH 10 CM GEL BOX, 2.0 MM THICKNESS
HGB10-25-2	CORNING	1/pk	EUR 59
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 25-WELL COMB FOR USE WITH 20 CM GEL BOX, 1.0 MM THICKNESS
HGB20-25-1	CORNING	1/pk	EUR 69
Description: Lab Equipment; Axygen Branded EQ

Recombinant Human Gelatin
7-05257	CHI Scientific	10mg	Ask for price

Recombinant Human Gelatin
7-05258	CHI Scientific	50mg	Ask for price

Recombinant Human Gelatin
7-05259	CHI Scientific	100mg	Ask for price

Gelatin (4%), Acidulated
GSA125	ScyTek Laboratories	125 ml	EUR 90

Gelatin (4%), Acidulated
GSA500	ScyTek Laboratories	500 ml	EUR 143

MTBK_20640-25
AR02-P0011-25	Abfrontier	25ug	EUR 473

Nucleotide Set 100mM (4 x 100 mM)
110-010	GeneOn	4 x 100 µl	EUR 56

Nucleotide Set 100mM (4 x 100 mM)
110-011	GeneOn	4 x 200 µl	EUR 68

Cover Glasses, Siliconized, 22 x 40 mm
C014	Insitus Biotechnologies	1 oz	EUR 182

Cover Glasses, Siliconized, 22 x 60 mm
C015	Insitus Biotechnologies	1 oz	EUR 191

Coumarin 343 X azide, 25 mg
42630	lumiprobe	25 mg	EUR 316

CA Syringe Filter, 0.22 µm, 25 mm, Nonsterile
M4219-100	Biovision		EUR 294

CA Syringe Filter, 0.22 µm, 25 mm, Nonsterile
M4220-100	Biovision		EUR 320

CA Syringe Filter, 0.45 µm, 25 mm, Nonsterile
M4224-100	Biovision		EUR 294

CA Syringe Filter, 0.45 µm, 25 mm, Nonsterile
M4225-100	Biovision		EUR 294

CA Syringe Filter, 0.45 µm, 25 mm, Nonsterile
M4226-100	Biovision		EUR 294

CA Syringe Filter, 1 µm, 25 mm, Nonsterile
M4229-100	Biovision		EUR 294

CA Syringe Filter, 3 µm, 25 mm, Nonsterile
M4231-100	Biovision		EUR 294

CA Syringe Filter, 5 µm, 25 mm, Nonsterile
M4234-100	Biovision		EUR 294

Glass Fiber Syringe Filter, 0.22 µm, 25 mm
M4238-100	Biovision		EUR 294

Glass Fiber Syringe Filter, 0.45 µm, 25 mm
M4240-100	Biovision		EUR 294

Glass Fiber Syringe Filter, 0.70 µm, 25 mm
M4242-100	Biovision		EUR 294

Glass Fiber Syringe Filter, 1 µm, 25 mm
M4244-100	Biovision		EUR 294

Glass Fiber Syringe Filter, 1.0 µm, 25 mm
M4246-100	Biovision		EUR 376

Glass Fiber Syringe Filter, 5 µm, 25 mm
M4247-100	Biovision		EUR 294

Glass Fiber Syringe Filter, 10 µm, 25 mm
M4248-100	Biovision		EUR 294

Nylon Syringe Filter, 0.10 µm, 25 mm, Nonsterile
M4269-100	Biovision		EUR 294

Nylon Syringe Filter, 0.22 µm, 25 mm, Nonsterile
M4274-100	Biovision		EUR 294

Nylon Syringe Filter, 0.22 µm, 25 mm, Nonsterile
M4275-100	Biovision		EUR 294

Nylon Syringe Filter, 0.22 µm, 25 mm, Nonsterile
M4277-100	Biovision		EUR 376

Nylon Syringe Filter, 0.45 µm, 25 mm, Nonsterile
M4282-100	Biovision		EUR 376

Nylon Syringe Filter, 0.45 µm, 25 mm, Nonsterile
M4283-100	Biovision		EUR 294

Nylon Syringe Filter, 0.45 µm, 25 mm, Nonsterile
M4284-100	Biovision		EUR 294

Nylon Syringe Filter, 0.45 µm, 25 mm, Nonsterile
M4285-100	Biovision		EUR 316

Nylon Syringe Filter, 1 µm, 25 mm, Nonsterile
M4289-100	Biovision		EUR 294

Nylon Syringe Filter, 1 µm, 25 mm, Nonsterile
M4290-100	Biovision		EUR 294

Nylon Syringe Filter, 3 µm, 25 mm, Nonsterile
M4293-100	Biovision		EUR 316

Nylon Syringe Filters, 3 µm, 25 mm, Nonsterile
M4294-100	Biovision		EUR 294

Nylon Syringe Filters, 5 µm, 25 mm, Nonsterile
M4297-100	Biovision		EUR 294

Nylon Syringe Filters, 5 µm, 25 mm, Nonsterile
M4298-100	Biovision		EUR 294

Nylon Syringe Filters, 10 µm, 25 mm, Nonsterile
M4299-100	Biovision		EUR 294

Nylon Syringe Filters, 10 µm, 25 mm, Nonsterile
M4300-100	Biovision		EUR 294

Nylon Syringe Filters, 5.0 µm, 25 mm, Nonsterile
M4301-100	Biovision		EUR 376

Polyethersulfone Syringe Filters, 0.22 µm, 25 mm, Sterile
M4303-100	Biovision		EUR 370

Polyethersulfone Syringe Filters, 0.22 µm, 25 mm, Sterile
M4303-200	Biovision		EUR 588

Polyethersulfone Syringe Filters, 0.45 µm, 25 mm, Sterile
M4306-100	Biovision		EUR 370

Polyethersulfone Syringe Filters, 0.45 µm, 25 mm, Sterile
M4306-200	Biovision		EUR 588

PES Syringe Filters, 0.22 um, 25 mm, Nonsterile
M4332-100	Biovision		EUR 316

PES Syringe Filters, 0.45 um, 25 mm, Nonsterile
M4339-100	Biovision		EUR 316

RPMI 1640, with 25 mM HEPES & L-Glutamine
CCM1606-500	Bio Basic	500 mL	EUR 62.85
Product category: Culture Media

Rv0351 (GrpE)-25
AR02-P0003-25	Abfrontier	25ug	EUR 126

Rv1009 (RpfB)-25
AR02-P0006-25	Abfrontier	25ug	EUR 473

Rv2450c (RpfE)-25
AR02-P0007-25	Abfrontier	25ug	EUR 473

Rv3875 (esxA)-25
AR02-P0008-25	Abfrontier	25ug	EUR 473

Rv2031c(HspX)-25
AR02-P0009-25	Abfrontier	25ug	EUR 473

Rv3131 (NfnB)-25
AR02-P0010-25	Abfrontier	25ug	EUR 473

Mini gel tubes, 7 x 2.5 x 100 mm, 8/pk
2394132	Atto	6unit	EUR 273

26795 25 CLOSURE NTRL 25MM
26795-25	CORNING	50/pk	EUR 96
Description: Disposable Screw Cap Culture Tubes; Poly Closures

Self-Quenched BODIPY Gelatin
7935-30	Biovision		EUR 153

PANCREATIC DIGEST OF GELATIN
P16-100-10kg	Alphabiosciences	10 kg	EUR 689

PANCREATIC DIGEST OF GELATIN
P16-100-2kg	Alphabiosciences	2kg	EUR 189

PANCREATIC DIGEST OF GELATIN
P16-100-500g	Alphabiosciences	500 g	EUR 88

Gelatin, Fluorescein (FITC) Conjugate
M1303-1	Biovision		EUR 98

Gelatin, Fluorescein (FITC) Conjugate
M1303-5	Biovision		EUR 218

Recombinant (yeast) Human Gelatin
RP-921	Alpha Diagnostics	5 mg	EUR 286

Peptone E GELATIN Peptone
G212	Bio Basic	100g	EUR 63.92
Product category: Culture Media/Supplements

X-press Tag Peptide
A6010-25	ApexBio	25 mg	EUR 587
Description: X-press Tag is an N-terminal leader peptide; this N-terminal peptide contains a polyhistidine sequence, the Xpress epitope (part of bacteriophage T7 gene 10?protein) and an enterokinase cleavage site. Anti-Xpress antibodies recognize the Xpress epitope sequence found in this leader peptide.

125 ML SPINNER FLASK, 70 MM CENTRE NECK, 2 X 32 MM SIDEARMS
4500-125	CORNING	1/pk	EUR 485
Description: Bioprocess Vessels; Spinner Flask 70

15 LTR SPINNER FLASK, 100 MM CENTRE NECK, 2 X 45 MM SIDEARMS
4500-15L	CORNING	1/pk	EUR 1459
Description: Bioprocess Vessels; Spinner Flask 70

1 LTR SPINNER FLASK, 100 MM CENTRE NECK, 2 X 45 MM SIDEARMS
4500-1L	CORNING	1/pk	EUR 748
Description: Bioprocess Vessels; Spinner Flask 70

We discuss the trade-offs and practical design rules to follow to develop a microscope DH by the proposed solution. Using our prototype, we picture flowing marine microalgae, beads of polystyrene, E.coli and microplastics. We detail the effects on the performance and cost of each of the parameters, design and hardware selection, guiding the reader toward the realization of optimized device that can be used out of the lab by non-expert users for point of care testing.