Polarization holographic microscope slide for birefringence imaging of anisotropic samples in microfluidics.

Birefringence is an important optical properties of anisotropic materials arising from the anisotropy of tissue microstructure. birefringence parameters have been found to be important to understand the architecture of many optical anisotropic material and polarization imaging has been applied in many fields of research in biology and medicine. Here, we propose a scheme for the miniaturize dual-channel polarization interferometer optical holograms to create holographic polarization microscope slide (P-HMS) suitable for integrating with (LoC) systems microfluidic lab-on-a-chip.

olympus confocal

Based on the P-HMS combined with simple reconstruction algorithms described in the paper, we can not only simultaneously realize holographic imaging of two orthogonal polarization components of dynamic samples in microfluidic channels but also a quantitative measurement of 2D information birefringence, both including birefringence the phase retardation and orientation optik- axis. Chip interferometer allows for off-axis polarization dual-channel digital holographic recording uses only a single light beam without any beam splitter or mirror. General quasi lane configuration and self-aligned design also makes it tolerant to vibration and misalignment. This work on the P-HMS can play a positive role in promoting the application of imaging birefringence in LOC microfluidic technology.


holographic microscope appeared as a suitable tool for in situ diagnostics and environmental monitoring, providing high-throughput, label-free, quantitative imaging capabilities through a small and compact device. In-line holographic microscope can be realized with contained costs, trade of complexity in decision phase and is limited to rare samples.

Here we present a 3D printed, cost effective and portable field off-axis holographic microscope slide hologram based microfluidic concepts. Our scheme eliminates the complexity of the reconstruction process, such as non-recurring and retrieval phase can be obtained by hologram demodulation. Our configuration ensures introduce flexibility in the definition of the optical scheme, exploited to realize a modular device with different features.

 Polarization holographic microscope slide for birefringence imaging of anisotropic samples in microfluidics.
Polarization holographic microscope slide for birefringence imaging of anisotropic samples in microfluidics.

A micro cell culture method utilizes modified microscope slides for use in fluorescent antibody and enzyme immunoassay.

A technique for the culture of a small number of mammalian cells on a microscope slide modified explained. The modified microscope slide is Bellco Glass, Inc., toxoplasmosis slides and cell culture used early part cow embryo lung cells and continuous cell lines origin pigs and dogs. Shear cell culture either infected or infected with a virus selected or obligate intracellular protozoanEncephalitozoon caniculi for use in the direct fluorescent antibody test and the indirect or peroxidase antiperoxidase immunosorbant test.

25-place microscope slide box (for 75 x 25 mm slides)

PO202 each
EUR 73.2

100-place microscope slide box (for 75 x 25 mm slides)

PO201 each
EUR 84

100-place microscope slide box (for 75 x 50 mm slides)

PO203 each
EUR 152.4

Microscope Slides, Plain

07441-1 1box
EUR 50

Microscope Slides, Micropure

22245-20 1box
EUR 380

Microscope Slides, Micropure

22245-1 1box
EUR 32

Box for 25 microscope slides purple colour

DD49719 EACH
EUR 19.2

Box for 25 microscope slides orange colour

DD49720 EACH
EUR 19.2

Box for 25 microscope slides turquoise colour

DD49721 EACH
EUR 19.2

Premium Plain Glass Microscope Slides, 144 /pk, 1 mm thickness, 25 mm wide by 73 mm long

M-ACC-GSL9 200 TIPS
EUR 65
Description: Premium Plain Glass Microscope Slides, 144 /pk, 1 mm thickness, 25 mm wide by 73 mm long

Vinyl microscope slides RINZL

DD52951 PK144
EUR 24.62

Microscope slide dispenser for box of 72 standard 25 x 75mm slides

M7100-SD 1/pack
EUR 83.89
Description: Microscope slide dispenser

Super Frosted Microscope Slides

21912-1 1box
EUR 60

Microscope Slides, Immunofluorescence

18357-1 1box
EUR 168

Microscope Slides Plain 1.0mm 76x52mm

MIC2156 PK50
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Superfrost® Plus microscope slides

PO103 box (72 slides)
EUR 123.6

Proteinase-resistant microscope slides

PO102 100 slides
EUR 236.4

Tissue Tack (+ Charged) Microscope Slides

24216-1 1box
EUR 55

Tissue Tack (+ Charged) Microscope Slides

24216-20 20box
EUR 720

Microscope Slides Plain 1.2-1.5mm 76x26mm

MIC2154 PK50
EUR 4.08

Microscope slides, positive charged, 45° corners, frosted on one end, 25 x 75mm

M7100-45 72/pack
EUR 75.4
Description: Microscope slides positive charged

Microscope slides, positive charged, 90° corners, frosted on one end, 25 x 75mm

M7100-90 72/pack
EUR 75.4
Description: Microscope slides positive charged

Cardboard tray for 20 microscope slides

DD65205 EACH
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TruBond 380 Adhesive Microscope Slides, 1000 pcs/case

IW-T380C -
EUR 385

TruBond 380 Adhesive Microscope Slides, 100 pcs/box

IW-T380 -
EUR 45

Quartz Microscope Slide, 1"x2" (25.4 x 50.8 mm) x 0.5 mm Thin

24962-1 1EA
EUR 217

Quartz Microscope Slide, 1"x3" (25.4 x 76.2 mm) x 0.5 mm Thin

24963-1 1EA
EUR 192

Quartz Microscope Slide, 1"x1" (25.4 x 25.4 mm) x 1 mm Thick

24957-1 1EA
EUR 58

Plastic box for 100 microscope slides (red colour)

DD37503 EACH
EUR 38.4

Plastic box for 100 microscope slides (blue colour)

DD37501 EACH
EUR 38.4

Plastic box for 50 microscope slides (blue colour)

DD37507 EACH
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Polystyrene box for 100 microscope slides blue colour

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EUR 22.8

Polystyrene box for 100 microscope slides green colour

DD139538 EACH
EUR 22.8

Microscope Slide Box 25 ULT safe

MIC4092 EACH
EUR 19.37

Plastic box for 100 microscope slides (assorted colours) cork bottom

DD10197 PK5
EUR 87.6

Microscope Slide Boxes,25-Place, Red

LC7009-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Red

LC7009-010 10/PK
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Microscope Slide Boxes,25-Place, Red

LC7009-100 100/CS
EUR 684

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EUR 79.2

Microscope Slide Boxes,25-Place, Blue

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Microscope Slide Boxes,25-Place, Blue

LC7007-100 100/CS
EUR 684

Microscope Slide Boxes,25-Place, Green

LC7008-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Green

LC7008-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Green

LC7008-100 100/CS
EUR 684

Microscope Slide Boxes,25-Place, White

LC7011-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, White

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EUR 148.8

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Microscope Slide Boxes,25-Place, Yellow

LC7010-001 EA
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Microscope Slide Boxes,25-Place, Yellow

LC7010-010 10/PK
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Microscope Slide Boxes,25-Place, Yellow

LC7010-100 100/CS
EUR 684

Microscope Slides 26X76mm Ground Edges Double Frosted 45° Angle -Silver-

VBS656 PK2500
EUR 262.8

AutoFrost Enhanced IHC Microscope Slides, Clipped corner, White, 3000pcs/case

EMP00W3 -
EUR 1250

Microscope Slide Coverslips, Glass, 22mm x 30mm

23999-1 1oz
EUR 40

Microscope Slide Coverslips, Glass, 24mm x 40mm

24070-1 1oz
EUR 40

Microscope Slide Coverslips, Glass, 24mm x 50mm

24071-1 1oz
EUR 41

Microscope Slide Coverslips, Glass, 24mm x 60mm

24072-1 1oz
EUR 53

Microscope Slide Coverslips, Glass, 22mm x 22mm

21913-1 1oz
EUR 40

Microscope Slide Holder Block

24764-1 1each
EUR 148

Microscope Slide Mailing Tubes

23998-10 10pkg
EUR 40

Microscope Slide Storage Cabinet

22352-1 1each
EUR 278

Microscope Slide Box True North

MIC4090 EACH
EUR 47.52

Microscope Slide Polysine 75x25x1mm

MIC3050 PK72
EUR 43.22

Microscope Slide Coverslips, Plastic

16601-1 1box
EUR 148

Microscope Slide Box 100 Place Tall

MIC4080 EACH
EUR 42

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BR474701-2500EA PK2500
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LC7002-010 10/PK
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Microscope Slide Boxes,100-Place, Blue

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19801A-1 1Box
EUR 56

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LC7003-010 10/PK
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Microscope Slide Boxes,100-Place, Green

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Microscope Slide Boxes,100-Place, White

LC7006-100 100/CS
EUR 967.2

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LC7005-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, Yellow

LC7005-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, Yellow

LC7005-100 100/CS
EUR 967.2

Microscope Slide Storage Cabinet Base Unit

22353-1 1each
EUR 397

2 Place Microscope Slide Mailer PP Natural

MIC3172 PK25
EUR 46.92

OOSB00004-72U - Gelatin Subbed Slides

OOSB00004-72U 72Slides/Box
EUR 109

OOSB00005-10U - Gelatin Subbed Slides

OOSB00005-10U 10Boxes/Case
EUR 819

Microscope Slide Storage Box, Plastic small box

16603-1 1bag
EUR 28

Microscope Slide Storage Box, Plastic large box

16604-1 1each
EUR 54

Premium Microscope Slide Box 50-Place Cork Red

MIC4216 EACH
EUR 21.46

Premium Microscope Slide Box 50-Place Cork Blue

MIC4214 EACH
EUR 26.52

2 Place Microscope Slide Mailer PP Assorted Colours

MIC3170 PK25
EUR 48

FlyStuff Microscope

59-502 1 Microscope/Unit
EUR 1801.67
Description: Binocular, w/base

Microscope Cover Slips No. 1.5 16mm

MIC2182 PK100
EUR 10.8

Microscope Cover Slips No. 0 18x18mm

MIC2158 PK100
EUR 3.6

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MIC2160 PK100
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Microscope Cover Slips No. 1 18x18mm

MIC2162 PK100
EUR 2.4

Microscope Cover Slips No. 1 22x22mm

MIC2164 PK100
EUR 3.6

Microscope Cover Slips No. 1.5 18x18mm

MIC2166 PK100
EUR 2.4

Microscope Cover Slips No. 1.5 22x22mm

MIC2168 PK100
EUR 3.6

Microscope Cover Slips No.1.5 22x26mm

MIC2170 PK100
EUR 4.8

Microscope Cover Slips No. 1.5 22x32mm

MIC2172 PK100
EUR 4.8

Microscope Cover Slips No. 1.5 22x40mm

MIC2174 PK100
EUR 6

Microscope Cover Slips No. 2 18x18mm

MIC2176 PK100
EUR 3.6

Microscope Cover Slips No. 2 22x22mm

MIC2178 PK100
EUR 4.8

Microscope Cover Slips No. 0 19mm dia

MIC2180 PK100
EUR 12

Stereo Microscope Trinocular

MIC1590 EACH
EUR 1274.27

FlyStuff Trinocular Microscope

59-503 1 Microscope/Unit
EUR 2779.21
Description: Trinocular, w/base

Upright Fluorescence Microscope

25039-1 1EA
EUR 19098

Inverted Fluorescence Microscope

25040-1 1EA
EUR 15134

BA310 Elite Trinocular microscope

MIC2937 EACH
EUR 2667.34

BA210E Elite Binocular Microscope

MIC5633 EACH
EUR 1809.56

Motic SMZ-168-BL Microscope

MIC2800 EACH
EUR 1357.74

Microscope Pack 1 CX23 100x Oil

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EUR 1827.42

Microscope Pack 2 CX23 BIN LED

MIC1970 EACH
EUR 1658.11

Digi-Pad Microscope Tablet Camera

MIC0115 EACH
EUR 1807.2

ReadiUseâ„¢ microscope mounting solution

20009-50mL 50 mL
EUR 86
Description: The mounting medium is the solution in which the specimen is embedded, generally under a cover glass.

Motic BA210 LED Microscope Binocular

MIC2004 EACH
EUR 1667.25

Motic BA310 LED Microscope Binocular

MIC2016 EACH
EUR 2250.85

Motic BA310 LED Microscope Trinocular

MIC2018 EACH
EUR 2545.92

VIKEM(R) VINYL MICROSCOPE COVER EXTRA

BAF243040000-1EA EACH
EUR 32.88

MIGHTY SCOPE(TM) DIGITAL MICROSCOPE 5M

Z742668-1EA EACH
EUR 451.74

ReadiUse™ microscope mounting solution

20009 50 mL
EUR 86

Motic SMZ-140-N2GG Stereo Microscope

MIC5900 EACH
EUR 774.22

Motic SMZ-143-N2GG Stereo Microscope

MIC5902 EACH
EUR 900.86

SMZ-171-Binocular P Stero Microscope

MIC5446 EACH
EUR 1552.36

Microscope Binoc MAGNUM-B planachrom obj

MIC0105 EACH
EUR 2008.8

SMZ-171-Binocular LED Stero Microscope

MIC5444 EACH
EUR 1989.73

SMZ-171-Trinocular P Stero Microscope

MIC5447 EACH
EUR 1668.56

Microscope Trinoc MAGNUM-T planachrom obj

MIC0107 EACH
EUR 2085.6

SMZ-171-Trinocular LED Stero Microscope

MIC5445 EACH
EUR 2108.54

BD BioCoat Fibronectin Coated 4 Well Tissue Culture Slides

354559 PK12
EUR 586.8

BD BioCoat Fibronectin Coated 8 Well Tissue Culture Slides

354631 PK12
EUR 514.8

BD BioCoat Collagen I Coated 4 Well Tissue Culture Slides

354557 PK12
EUR 573.6

BD BioCoat Collagen I Coated 8 Well Tissue Culture Slides

354630 PK12
EUR 465.6

Microscope Binoc MAGNUM-B pos phase contrast

MIC0106 EACH
EUR 2624.4

Streptavidin Coated 12 x 75 mm Tubes

TS1275-SA5/500 1 pack = 5 units of 20 pcs
EUR 268.18

Streptavidin Coated 12 x 75 mm Tubes

TS1275-SA5/500/W 1 pack = 5 units of 20 pcs
EUR 326.48

SLS Lab Basics Star-24 LED Stereo Microscope

MIC2026 EACH
EUR 1422

Microscope Trinoc MAGNUM-T pos phase contrast

MIC0108 EACH
EUR 2865.6

Gelatin Coating Solution (0.1%)

TM063 100 ml
EUR 100

MiTeGen Kit V201 Stereo Microscope kit for MicroCrystallography:

ZV20-MTGKV201 1 KIT
EUR 16532
Description: MiTeGen Kit V201 Stereo Microscope kit for MicroCrystallography:- Ergonomic adjustable eyepieces- A wide array of accessories available- 7.5 to 150 x magnification range- ZEISS SteREO Discovery.V20- ZEISS SteREO Discovery.V20 body,- Human Interface Panel HIP,- Dust Protection Set M ,- Binoc Phototube Ergo Stereo 5-45 ,- Eyepiece PL 10x/23 Br foc ,- Folding Eyecup ,- Stand base Profile S,- Transmitted Light Equipment S- Cold-light source Zeiss CL6000 LED- 6200K color temperature,- control of intensity & 6 memory positions,- filter slider for 2 filters 35x26x4mm (sold separately)- Analyzer S Rotatable,- Polarizer D =84mm,- Coarse/fine drive w/column S 490mm- Mount S with 76 mm Diameter Support- Achromat S 1.0x Reo WD=63 lens

MiTeGen Kit V81 Stereo Microscope kit for Crystallography:

ZV20-MTGKV81 1 KIT
EUR 13998
Description: MiTeGen Kit V81 Stereo Microscope kit for Crystallography:- ZEISS Stereo Discovery V8 Microscope:- ZEISS Stereo Discovery.V8 body,- Dust Protection Set M ,- Binocular Phototube Ergo Stereo 5-45 ,- Eyepiece PL 10x/23 Br foc ,- Folding Eyecup ,- Stand base Profile S,- Transmitted Light Equipment S:- Cold-light source ZEISS CL6000 LED- 6200K color temperature, control of intensity & 6 memory positions,- filter slider for 2 filters (35 x 26 x 4mm, (filters sold separately)- Analyzer S Rotatable,- Polarizer D =84mm,- Manual focus drive fa/ Discovery,- Mount S with 76 mm Diameter Support- Achromatic S 1.0x Reo WD=63 lens

MiTeGen Kit V121 Stereo Microscope kit for Crystallography:

ZV20-MTGKV121 1 KIT
EUR 15282
Description: MiTeGen Kit V121 Stereo Microscope kit for Crystallography:- ZEISS Stereo Discovery V12 Microscope:- ZEISS Stereo Discovery.V8 body,- Dust Protection Set M ,- Binocular Phototube Ergo Stereo 5-45 ,- Eyepiece PL 10x/23 Br foc ,- Folding Eyecup ,- Stand base Profile S,- Transmitted Light Equipment S:- Cold-light source ZEISS CL6000 LED- 6200K color temperature,- control of intensity & 6 memory positions,- filter slider for 2 filters (35 x 26 x 4mm, (filters sold separately)- Analyzer S Rotatable,- Polarizer D =84mm,- Manual focus drive fa/ Discovery,- Mount S with 76 mm Diameter Support- Achromatic S 1.0x Reo WD=63 lens

MiTeGen Kit Stemi 305 Stereo Microscope kit for Crystallography:

ZV20-MTGK305 1 KIT
EUR 2589
Description: MiTeGen Kit Stemi 305 Stereo Microscope kit for Crystallography:Stemi 305 trinocular body w/ integrated camera portIncludes adapter for C-mount lensesStand K Edu w/ integrated transmissive LED cold light and polarizerSpot illuminator w/ polarizerAnalyzer (rotatable)

MiTeGen Crystallography Microscope Kit for Protein Crystallography:

ZV20-MTGK508-PC1 1 KIT
EUR 6492
Description: MiTeGen Crystallography Microscope Kit for Protein Crystallography:- Stemi 508 Stand M- Magnification: 10-80x (with 16x eyepieces)- Free Working Distance: 92 mm- Eyepieces (2 x 10x & 2 x 16x)- Cold light (LED) transillumination source with large working area stand- Rotatable and slidable mirror for brightfield, darkfield and oblique transillumination- Plain mirror side for crisp, diffuse mirror side for homogeneous illumination- Trinocular Camera Port Configuration- Camera Adapter 60N- Viewing angle 35 degrees with adjustable interocular distance- Camera Port- Analyzer (rotatable)- Transillumination Polarizer- Dual flex-neck top-side LED illumination

Max III Trinocular Compound Microscope with Achromatic Objectives

SLS2270 EACH
EUR 1194

Max III Trinocular Compound Microscope with Plan Achromatic Objectives

SLS2272 EACH
EUR 1525.2

Streptavidin High Binding Coated 12 x 75 mm Tubes

TS1275-SB75/500 1 pack = 5 units of 20 pcs
EUR 303.16

Streptavidin High Binding Coated 12 x 75 mm Tubes

TS1275-SB75/500/W 1 pack = 5 units of 20 pcs
EUR 373.12

Steddy-T Trinocular Stereo Microscope with Diamond Base with Zoom Ratio 0.67x - 4.5x

SLS2106 EACH
EUR 1638

Biotin Coated Microspheres, CP10N, 10µm

CP10000-1 1 mL
EUR 229
Description: HS Code : 38220000

Biotin Coated Microspheres, CP10N, 10µm

CP10000-10 10 mL
EUR 1071
Description: HS Code : 38220000

Biotin Coated Microspheres, CP10N, 10µm

CP10000-2 2 mL
EUR 357
Description: HS Code : 38220000

Biotin Coated Microspheres, CP10N, 10µm

CP10000-5 5 mL
EUR 644
Description: HS Code : 38220000

Magtex Trinocular Materials and Biological Microscope with Plan Achromatic Objectives

SLS2280 EACH
EUR 4160.4

Gelatin

abx082013-100g 100 g
EUR 243.6

GELATIN

G07-102-10kg 10 kg
EUR 627.6

GELATIN

G07-102-2kg 2kg
EUR 183.6

GELATIN

G07-102-500g 500 g
EUR 93.6

Gelatin

G9764 100g
EUR 75.66

Gelatin

16605-42 25G
EUR 9.1

Gelatin

16605-55 500G
EUR 19.6

We discuss the trade-offs and practical design rules to follow to develop a microscope DH by the proposed solution. Using our prototype, we picture flowing marine microalgae, beads of polystyrene, E.coli and microplastics. We detail the effects on the performance and cost of each of the parameters, design and hardware selection, guiding the reader toward the realization of optimized device that can be used out of the lab by non-expert users for point of care testing.