Polarization holographic microscope slide for birefringence imaging of anisotropic samples in microfluidics.

Birefringence is an important optical properties of anisotropic materials arising from the anisotropy of tissue microstructure. birefringence parameters have been found to be important to understand the architecture of many optical anisotropic material and polarization imaging has been applied in many fields of research in biology and medicine. Here, we propose a scheme for the miniaturize dual-channel polarization interferometer optical holograms to create holographic polarization microscope slide (P-HMS) suitable for integrating with (LoC) systems microfluidic lab-on-a-chip.

olympus confocal

Based on the P-HMS combined with simple reconstruction algorithms described in the paper, we can not only simultaneously realize holographic imaging of two orthogonal polarization components of dynamic samples in microfluidic channels but also a quantitative measurement of 2D information birefringence, both including birefringence the phase retardation and orientation optik- axis. Chip interferometer allows for off-axis polarization dual-channel digital holographic recording uses only a single light beam without any beam splitter or mirror. General quasi lane configuration and self-aligned design also makes it tolerant to vibration and misalignment. This work on the P-HMS can play a positive role in promoting the application of imaging birefringence in LOC microfluidic technology.


holographic microscope appeared as a suitable tool for in situ diagnostics and environmental monitoring, providing high-throughput, label-free, quantitative imaging capabilities through a small and compact device. In-line holographic microscope can be realized with contained costs, trade of complexity in decision phase and is limited to rare samples.

Here we present a 3D printed, cost effective and portable field off-axis holographic microscope slide hologram based microfluidic concepts. Our scheme eliminates the complexity of the reconstruction process, such as non-recurring and retrieval phase can be obtained by hologram demodulation. Our configuration ensures introduce flexibility in the definition of the optical scheme, exploited to realize a modular device with different features.

 Polarization holographic microscope slide for birefringence imaging of anisotropic samples in microfluidics.
Polarization holographic microscope slide for birefringence imaging of anisotropic samples in microfluidics.

A micro cell culture method utilizes modified microscope slides for use in fluorescent antibody and enzyme immunoassay.

A technique for the culture of a small number of mammalian cells on a microscope slide modified explained. The modified microscope slide is Bellco Glass, Inc., toxoplasmosis slides and cell culture used early part cow embryo lung cells and continuous cell lines origin pigs and dogs. Shear cell culture either infected or infected with a virus selected or obligate intracellular protozoanEncephalitozoon caniculi for use in the direct fluorescent antibody test and the indirect or peroxidase antiperoxidase immunosorbant test.

25-place microscope slide box (for 75 x 25 mm slides)

PO202 each
EUR 59.1
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100-place microscope slide box (for 75 x 25 mm slides)

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EUR 67.03
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Description: Reusable Plastics; Reusable Funnels

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Description: Microscope slide dispenser

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EUR 77
Description: Micro Tubes; Screw Cap Macro Tubes - Axygen

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Description: Micro Tubes; Screw Cap Macro Tubes - Axygen

Mini gel tubes, 7 x 2.5 x 75 mm, 8/pk

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Oligo (dT)25 Coated Gold Particles

OdT(25)AuP-002-1 1 mL
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EUR 1045
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DiagNano Fluorophore Labeled Gold Nanoparticles, 75 nm

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MgCl2 25 mM

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EUR 62
Description: High purity buffer for various PCR applications.

Microscope Slide Boxes,25-Place, Blue

LC7007-001 EA
EUR 66

Microscope Slide Boxes,25-Place, Blue

LC7007-010 10/PK
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Microscope Slide Boxes,25-Place, Blue

LC7007-100 100/CS
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EUR 570

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EUR 92

COVER GLASS, NO. 2, SQUARE 25 X 25MM

2855-25 50/pk
EUR 86
Description: Cover Glass; Cover Glass, CGW

COVER GLASS, NO. 1, SQUARE 25 X 25MM

2845-25 100/pk
EUR 112
Description: Cover Glass; Cover Glass, CGW

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TCS0003-25 25 Slides
EUR 232

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TCS0007-25 25 Slides
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TCS0008-25 25 Slides
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TCS0015-25 25 Slides
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TCS0016-25 25 Slides
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TCS0019-25 25 Slides
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2947 75 X 25MM

2947-75X25 0,5/pk
EUR 248
Description: Slides; Slides

2947 75 X 38MM

2947-75X38 0,5/pk
EUR 209
Description: Slides; Slides

2947 75 X 50MM

2947-75X50 0,5/pk
EUR 265
Description: Slides; Slides

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2948-75X25 0,5/pk
EUR 317
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2949 75 X 25MM

2949-75X25 0,5/pk
EUR 405
Description: Slides; Slides

Gelatin

abx082013-100g 100 g
EUR 203
  • Shipped within 5-10 working days.

GELATIN

G07-102-10kg 10 kg
EUR 523

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G07-102-2kg 2kg
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OdT25P-100-4 4 mL
EUR 411
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Alcian Blue (Control Slides)

TCS0002-25 25 Slides
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MM-102

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EUR 588

MM 11253

B7505-25 25 mg
EUR 503

COVER GLASS, NO. 1 1/2, SQUARE 25 X 25MM

2850-25 100/pk
EUR 227
Description: Cover Glass; Cover Glass, CGW

MgCl2 25 mM 1.8 ml

BA00153 1.8ml
EUR 55
Description: High purity buffer for various PCR applications.

1.1 MM X 4 MM GEL CUTTING TIPS

TGL-1140 250/pk
EUR 298
Description: Non Filter Tips; Pipette Tips - Axygen

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Description: Non Filter Tips; Pipette Tips - Axygen

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EUR 106
Description: Minimum order quantity: 1 unit of 100G

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EUR 236

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  • Area of application: Hepatitis testing
Description: ELISA based test for quantitative detection of HAV antibody IgM

mt comb 25 sample, 1.0 mm

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EUR 65

mt comb 25 sample, 1.5 mm

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PAS/Glycogen, For Digestion (Control Slides)

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EUR 232

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EUR 485

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CD Marker Multi-Tumor control slides

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AXYGEN® 25-WELL COMB FOR USE WITH 10 CM GEL BOX, 1.0 MM THICKNESS

HGB10-25-1 1/pk
EUR 59
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 25-WELL COMB FOR USE WITH 10 CM GEL BOX, 2.0 MM THICKNESS

HGB10-25-2 1/pk
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Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 25-WELL COMB FOR USE WITH 20 CM GEL BOX, 1.0 MM THICKNESS

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EUR 69
Description: Lab Equipment; Axygen Branded EQ

Recombinant Human Gelatin

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Recombinant Human Gelatin

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EUR 90

Gelatin (4%), Acidulated

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EUR 56

We discuss the trade-offs and practical design rules to follow to develop a microscope DH by the proposed solution. Using our prototype, we picture flowing marine microalgae, beads of polystyrene, E.coli and microplastics. We detail the effects on the performance and cost of each of the parameters, design and hardware selection, guiding the reader toward the realization of optimized device that can be used out of the lab by non-expert users for point of care testing.