Polarization holographic microscope slide for birefringence imaging of anisotropic samples in microfluidics.

Birefringence is an important optical properties of anisotropic materials arising from the anisotropy of tissue microstructure. birefringence parameters have been found to be important to understand the architecture of many optical anisotropic material and polarization imaging has been applied in many fields of research in biology and medicine. Here, we propose a scheme for the miniaturize dual-channel polarization interferometer optical holograms to create holographic polarization microscope slide (P-HMS) suitable for integrating with (LoC) systems microfluidic lab-on-a-chip.

Based on the P-HMS combined with simple reconstruction algorithms described in the paper, we can not only simultaneously realize holographic imaging of two orthogonal polarization components of dynamic samples in microfluidic channels but also a quantitative measurement of 2D information birefringence, both including birefringence the phase retardation and orientation optik- axis. Chip interferometer allows for off-axis polarization dual-channel digital holographic recording uses only a single light beam without any beam splitter or mirror. General quasi lane configuration and self-aligned design also makes it tolerant to vibration and misalignment. This work on the P-HMS can play a positive role in promoting the application of imaging birefringence in LOC microfluidic technology.


holographic microscope appeared as a suitable tool for in situ diagnostics and environmental monitoring, providing high-throughput, label-free, quantitative imaging capabilities through a small and compact device. In-line holographic microscope can be realized with contained costs, trade of complexity in decision phase and is limited to rare samples.

Here we present a 3D printed, cost effective and portable field off-axis holographic microscope slide hologram based microfluidic concepts. Our scheme eliminates the complexity of the reconstruction process, such as non-recurring and retrieval phase can be obtained by hologram demodulation. Our configuration ensures introduce flexibility in the definition of the optical scheme, exploited to realize a modular device with different features.

 Polarization holographic microscope slide for birefringence imaging of anisotropic samples in microfluidics.
Polarization holographic microscope slide for birefringence imaging of anisotropic samples in microfluidics.

A micro cell culture method utilizes modified microscope slides for use in fluorescent antibody and enzyme immunoassay.

A technique for the culture of a small number of mammalian cells on a microscope slide modified explained. The modified microscope slide is Bellco Glass, Inc., toxoplasmosis slides and cell culture used early part cow embryo lung cells and continuous cell lines origin pigs and dogs. Shear cell culture either infected or infected with a virus selected or obligate intracellular protozoanEncephalitozoon caniculi for use in the direct fluorescent antibody test and the indirect or peroxidase antiperoxidase immunosorbant test.

25-place microscope slide box (for 75 x 25 mm slides)

PO202 each
EUR 59.10
Description: Best quality products

100-place microscope slide box (for 75 x 25 mm slides)

PO201 each
EUR 67.03
Description: Best quality products

100-place microscope slide box (for 75 x 50 mm slides)

PO203 each
EUR 114.36
Description: Best quality products

FUNNEL, 75 MM, PP

6120P-75 12/pk
EUR 40.00
Description: Reusable Plastics; Reusable Funnels

Proteinase-resistant microscope slides

PO102 100 slides
EUR 186.41
Description: Best quality products

Superfrost® Plus microscope slides

PO103 box (72 slides)
EUR 94.55
Description: Best quality products

12 X 75 MM POLYPROPYLENE CULTURE TUBE

T-1275-PP 500/pk
EUR 77.00
Description: Micro Tubes; Screw Cap Macro Tubes - Axygen

12 X 75 MM POLYSTYRENE CULTURE TUBE.

T-1275-PS 500/pk
EUR 78.00
Description: Micro Tubes; Screw Cap Macro Tubes - Axygen

DiagNano Fluorophore Labeled Gold Nanoparticles, 75 nm

GFL-75 1 mL
EUR 1053.00

Mini gel tubes, 7 x 2.5 x 75 mm, 8/pk

2394122 6unit
EUR 263.00

Oligo (dT)25 Coated Gold Particles

OdT(25)AuP-002-1 1 mL
EUR 385.00
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Oligo (dT)25 Coated Gold Particles

OdT(25)AuP-002-5 5 mL
EUR 1045.00
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MgCl2 25 mM

BA00154 5x1.8ml
EUR 62.00
Description: High purity buffer for various PCR applications.

Single channel mini-pipette (130 mm) 25 ul, autoclavable

SCMP-25 1
EUR 92.00

Microscope Slide Boxes,25-Place, Blue

LC7007-001 EA
EUR 66.00

Microscope Slide Boxes,25-Place, Blue

LC7007-010 10/PK
EUR 124.00

Microscope Slide Boxes,25-Place, Blue

LC7007-100 100/CS
EUR 570.00

Microscope Slide Boxes,25-Place, Green

LC7008-001 EA
EUR 66.00

Microscope Slide Boxes,25-Place, Green

LC7008-010 10/PK
EUR 124.00

Microscope Slide Boxes,25-Place, Green

LC7008-100 100/CS
EUR 570.00

Microscope Slide Boxes,25-Place, Red

LC7009-001 EA
EUR 66.00

Microscope Slide Boxes,25-Place, Red

LC7009-010 10/PK
EUR 124.00

Microscope Slide Boxes,25-Place, Red

LC7009-100 100/CS
EUR 570.00

Microscope Slide Boxes,25-Place, Yellow

LC7010-001 EA
EUR 66.00

Microscope Slide Boxes,25-Place, Yellow

LC7010-010 10/PK
EUR 124.00

Microscope Slide Boxes,25-Place, Yellow

LC7010-100 100/CS
EUR 570.00

Microscope Slide Boxes,25-Place, White

LC7011-001 EA
EUR 66.00

Microscope Slide Boxes,25-Place, White

LC7011-010 10/PK
EUR 124.00

Microscope Slide Boxes,25-Place, White

LC7011-100 100/CS
EUR 570.00

COVER GLASS, NO. 2, SQUARE 25 X 25MM

2855-25 50/pk
EUR 86.00
Description: Cover Glass; Cover Glass, CGW

COVER GLASS, NO. 1, SQUARE 25 X 25MM

2845-25 100/pk
EUR 112.00
Description: Cover Glass; Cover Glass, CGW

Amyloid (Control Slides)

TCS0003-25 25 Slides
EUR 232.00

Argentaffin (Control Slides)

TCS0004-25 25 Slides
EUR 232.00

Fungus (Control Slides)

TCS0005-25 25 Slides
EUR 232.00

Bielschowsky's (Control Slides)

TCS0006-25 25 Slides
EUR 232.00

Calcium (Control Slides)

TCS0007-25 25 Slides
EUR 232.00

Elastic (Control Slides)

TCS0008-25 25 Slides
EUR 232.00

Melanin (Control Slides)

TCS0015-25 25 Slides
EUR 232.00

Mucin (Control Slides)

TCS0016-25 25 Slides
EUR 232.00

Reticulum (Control Slides)

TCS0019-25 25 Slides
EUR 232.00

2947 75 X 25MM

2947-75X25 0,5/pk
EUR 248.00
Description: Slides; Slides

2947 75 X 38MM

2947-75X38 0,5/pk
EUR 209.00
Description: Slides; Slides

2947 75 X 50MM

2947-75X50 0,5/pk
EUR 265.00
Description: Slides; Slides

2948 75 X 25MM

2948-75X25 0,5/pk
EUR 317.00
Description: Slides; Slides

2949 75 X 25MM

2949-75X25 0,5/pk
EUR 405.00
Description: Slides; Slides

Gelatin

abx082013-100g 100 g
EUR 203.00
  • Shipped within 5-10 working days.

GELATIN

G07-102-10kg 10 kg
EUR 523.00

GELATIN

G07-102-2kg 2kg
EUR 153.00

GELATIN

G07-102-500g 500 g
EUR 78.00

Gelatin

G9764 100g
EUR 63.05
  • Product category: Culture Media/Other

MM-102

B2364-25
EUR 588.00

MM 11253

B7505-25 25 mg
EUR 503.00

Alcian Blue (Control Slides)

TCS0002-25 25 Slides
EUR 232.00

Helicobacter Pylori (Control Slides)

TCS0011-25 25 Slides
EUR 232.00

Iron - Liver (Control Slides)

TCS0013-25 25 Slides
EUR 232.00

Mast Cell (Control Slides)

TCS0014-25 25 Slides
EUR 232.00

PAS/Glycogen (Control Slides)

TCS0017-25 25 Slides
EUR 232.00

Trichrome - Colon (Control Slides)

TCS0021-25 25 Slides
EUR 232.00

Trichrome - Liver (Control Slides)

TCS0022-25 25 Slides
EUR 232.00

COVER GLASS, NO. 1 1/2, SQUARE 25 X 25MM

2850-25 100/pk
EUR 227.00
Description: Cover Glass; Cover Glass, CGW

MgCl2 25 mM 1.8 ml

BA00153 1.8ml
EUR 55.00
Description: High purity buffer for various PCR applications.

Oligo (dT)25 Coated Polystyrene Beads

OdT25P-100-4 4 mL
EUR 411.00
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1.1 MM X 4 MM GEL CUTTING TIPS

TGL-1140 250/pk
EUR 298.00
Description: Non Filter Tips; Pipette Tips - Axygen

1.1 MM X 4 MM GEL CUTTING TIPS

TGL-1140-R 480/pk
EUR 407.00
Description: Non Filter Tips; Pipette Tips - Axygen

1.1 MM X 6.5 MM GEL CUTTING TIPS

TGL-1165 250/pk
EUR 298.00
Description: Non Filter Tips; Pipette Tips - Axygen

1.1 MM X 6.5 MM GEL CUTTING TIPS

TGL-1165-R 480/pk
EUR 407.00
Description: Non Filter Tips; Pipette Tips - Axygen

100 mM Triton X-100

603 100 ml
EUR 98.00

HAV antibody IgM ELISA test

75 96T/Box Ask for price
  • Area of application: Hepatitis testing
Description: ELISA based test for quantitative detection of HAV antibody IgM

Fish Gelatin

22011 100G
EUR 106.00
Description: Minimum order quantity: 1 unit of 100G

NUTRIENT GELATIN

N14-108-10kg 10 kg
EUR 907.00

NUTRIENT GELATIN

N14-108-2kg 2kg
EUR 236.00

NUTRIENT GELATIN

N14-108-500g 500 g
EUR 101.00

PAS/Glycogen, For Digestion (Control Slides)

TCS0024-25 25 Slides
EUR 232.00

Core Panel Multi-Tumor control slides

TS900-25 Set of 25
EUR 485.00

Breast Panel Multi-Tumor control slides

TS901-25 Set of 25
EUR 485.00

CD Marker Multi-Tumor control slides

TS902-25 Set of 25
EUR 485.00

comb 1 mm thick 25 sample

EHS1200-C25-1.0 ea
EUR 41.00

comb 1.5 mm thick 25 sample

EHS1200-C25-1.5 ea
EUR 41.00

comb 1 mm thick 25 sample

EHS1400-C25-1.0 ea
EUR 56.00

comb 1.5 mm thick 25 sample

EHS1400-C25-1.5 ea
EUR 56.00

mt comb 25 sample, 1.0 mm

EHS3300-CMT25-1.0 ea
EUR 65.00

mt comb 25 sample, 1.5 mm

EHS3300-CMT25-1.5 ea
EUR 65.00

mt comb 25 sample, 1.0 mm

EHS3600-CMT25-1.0 ea
EUR 73.00

mt comb 25 sample, 1.5 mm

EHS3600-CMT25-1.5 ea
EUR 73.00

AXYGEN® 25-WELL COMB FOR USE WITH 10 CM GEL BOX, 1.0 MM THICKNESS

HGB10-25-1 1/pk
EUR 59.00
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 25-WELL COMB FOR USE WITH 10 CM GEL BOX, 2.0 MM THICKNESS

HGB10-25-2 1/pk
EUR 59.00
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 25-WELL COMB FOR USE WITH 20 CM GEL BOX, 1.0 MM THICKNESS

HGB20-25-1 1/pk
EUR 69.00
Description: Lab Equipment; Axygen Branded EQ

Recombinant Human Gelatin

7-05257 10mg Ask for price

Recombinant Human Gelatin

7-05258 50mg Ask for price

Recombinant Human Gelatin

7-05259 100mg Ask for price

Gelatin (4%), Acidulated

GSA125 125 ml
EUR 90.00

Gelatin (4%), Acidulated

GSA500 500 ml
EUR 143.00

MTBK_20640-25

AR02-P0011-25 25ug
EUR 473.00

Cover Glasses, Siliconized, 22 x 40 mm

C014 1 oz
EUR 182.00

Cover Glasses, Siliconized, 22 x 60 mm

C015 1 oz
EUR 191.00

Nucleotide Set 100mM (4 x 100 mM)

110-010 4 x 100 µl
EUR 56.00

Nucleotide Set 100mM (4 x 100 mM)

110-011 4 x 200 µl
EUR 68.00

Rv0351 (GrpE)-25

AR02-P0003-25 25ug
EUR 126.00

Rv1009 (RpfB)-25

AR02-P0006-25 25ug
EUR 473.00

Rv2450c (RpfE)-25

AR02-P0007-25 25ug
EUR 473.00

Rv3875 (esxA)-25

AR02-P0008-25 25ug
EUR 473.00

Rv2031c(HspX)-25

AR02-P0009-25 25ug
EUR 473.00

Rv3131 (NfnB)-25

AR02-P0010-25 25ug
EUR 473.00

RPMI 1640, with 25 mM HEPES & L-Glutamine

CCM1606-500 500 mL
EUR 62.85
  • Product category: Culture Media

CA Syringe Filter, 0.22 µm, 25 mm, Nonsterile

M4219-100
EUR 294.00

CA Syringe Filter, 0.22 µm, 25 mm, Nonsterile

M4220-100
EUR 320.00

CA Syringe Filter, 0.45 µm, 25 mm, Nonsterile

M4224-100
EUR 294.00

CA Syringe Filter, 0.45 µm, 25 mm, Nonsterile

M4225-100
EUR 294.00

CA Syringe Filter, 0.45 µm, 25 mm, Nonsterile

M4226-100
EUR 294.00

CA Syringe Filter, 1 µm, 25 mm, Nonsterile

M4229-100
EUR 294.00

CA Syringe Filter, 3 µm, 25 mm, Nonsterile

M4231-100
EUR 294.00

CA Syringe Filter, 5 µm, 25 mm, Nonsterile

M4234-100
EUR 294.00

Glass Fiber Syringe Filter, 0.22 µm, 25 mm

M4238-100
EUR 294.00

Glass Fiber Syringe Filter, 0.45 µm, 25 mm

M4240-100
EUR 294.00

Glass Fiber Syringe Filter, 0.70 µm, 25 mm

M4242-100
EUR 294.00

Glass Fiber Syringe Filter, 1 µm, 25 mm

M4244-100
EUR 294.00

Glass Fiber Syringe Filter, 1.0 µm, 25 mm

M4246-100
EUR 376.00

Glass Fiber Syringe Filter, 5 µm, 25 mm

M4247-100
EUR 294.00

Glass Fiber Syringe Filter, 10 µm, 25 mm

M4248-100
EUR 294.00

Nylon Syringe Filter, 0.10 µm, 25 mm, Nonsterile

M4269-100
EUR 294.00

Nylon Syringe Filter, 0.22 µm, 25 mm, Nonsterile

M4274-100
EUR 294.00

Nylon Syringe Filter, 0.22 µm, 25 mm, Nonsterile

M4275-100
EUR 294.00

Nylon Syringe Filter, 0.22 µm, 25 mm, Nonsterile

M4277-100
EUR 376.00

Nylon Syringe Filter, 0.45 µm, 25 mm, Nonsterile

M4282-100
EUR 376.00

Nylon Syringe Filter, 0.45 µm, 25 mm, Nonsterile

M4283-100
EUR 294.00

Nylon Syringe Filter, 0.45 µm, 25 mm, Nonsterile

M4284-100
EUR 294.00

Nylon Syringe Filter, 0.45 µm, 25 mm, Nonsterile

M4285-100
EUR 316.00

Nylon Syringe Filter, 1 µm, 25 mm, Nonsterile

M4289-100
EUR 294.00

Nylon Syringe Filter, 1 µm, 25 mm, Nonsterile

M4290-100
EUR 294.00

Nylon Syringe Filter, 3 µm, 25 mm, Nonsterile

M4293-100
EUR 316.00

Nylon Syringe Filters, 3 µm, 25 mm, Nonsterile

M4294-100
EUR 294.00

Nylon Syringe Filters, 5 µm, 25 mm, Nonsterile

M4297-100
EUR 294.00

Nylon Syringe Filters, 5 µm, 25 mm, Nonsterile

M4298-100
EUR 294.00

Nylon Syringe Filters, 10 µm, 25 mm, Nonsterile

M4299-100
EUR 294.00

Nylon Syringe Filters, 10 µm, 25 mm, Nonsterile

M4300-100
EUR 294.00

Nylon Syringe Filters, 5.0 µm, 25 mm, Nonsterile

M4301-100
EUR 376.00

Polyethersulfone Syringe Filters, 0.22 µm, 25 mm, Sterile

M4303-100
EUR 370.00

Polyethersulfone Syringe Filters, 0.22 µm, 25 mm, Sterile

M4303-200
EUR 588.00

Polyethersulfone Syringe Filters, 0.45 µm, 25 mm, Sterile

M4306-100
EUR 370.00

Polyethersulfone Syringe Filters, 0.45 µm, 25 mm, Sterile

M4306-200
EUR 588.00

PES Syringe Filters, 0.22 um, 25 mm, Nonsterile

M4332-100
EUR 316.00

PES Syringe Filters, 0.45 um, 25 mm, Nonsterile

M4339-100
EUR 316.00

26795 25 CLOSURE NTRL 25MM

26795-25 50/pk
EUR 96.00
Description: Disposable Screw Cap Culture Tubes; Poly Closures

Coumarin 343 X azide, 25 mg

42630 25 mg
EUR 316.00

Mini gel tubes, 7 x 2.5 x 100 mm, 8/pk

2394132 6unit
EUR 273.00

X-press Tag Peptide

A6010-25 25 mg
EUR 587.00
Description: X-press Tag is an N-terminal leader peptide; this N-terminal peptide contains a polyhistidine sequence, the Xpress epitope (part of bacteriophage T7 gene 10?protein) and an enterokinase cleavage site. Anti-Xpress antibodies recognize the Xpress epitope sequence found in this leader peptide.

Self-Quenched BODIPY Gelatin

7935-30
EUR 153.00

Peptone E GELATIN Peptone

G212 100g
EUR 63.92
  • Product category: Culture Media/Supplements

Gelatin, Fluorescein (FITC) Conjugate

M1303-1
EUR 98.00

Gelatin, Fluorescein (FITC) Conjugate

M1303-5
EUR 218.00

PANCREATIC DIGEST OF GELATIN

P16-100-10kg 10 kg
EUR 689.00

PANCREATIC DIGEST OF GELATIN

P16-100-2kg 2kg
EUR 189.00

PANCREATIC DIGEST OF GELATIN

P16-100-500g 500 g
EUR 88.00

Recombinant (yeast) Human Gelatin

RP-921 5 mg
EUR 286.00

Positive control tissue section for each antibody; Based on availability INQUIRE

Control-Slides Set of 5
EUR 176.00

100 ML-GLUTAMINE (200 MM, LIQUID)

25-005-CI 100 mL/pk
EUR 58.00
Description: Media Catalog; Cell Culture Reagents

125 ML SPINNER FLASK, 70 MM CENTRE NECK, 2 X 32 MM SIDEARMS

4500-125 1/pk
EUR 485.00
Description: Bioprocess Vessels; Spinner Flask 70

15 LTR SPINNER FLASK, 100 MM CENTRE NECK, 2 X 45 MM SIDEARMS

4500-15L 1/pk
EUR 1459.00
Description: Bioprocess Vessels; Spinner Flask 70

1 LTR SPINNER FLASK, 100 MM CENTRE NECK, 2 X 45 MM SIDEARMS

4500-1L 1/pk
EUR 748.00
Description: Bioprocess Vessels; Spinner Flask 70

36 LTR SPINNER FLASK, 100 MM CENTRE NECK, 2 X 45 MM SIDEARMS

4500-36L 1/pk
EUR 2118.00
Description: Bioprocess Vessels; Spinner Flask 70

3 LTR SPINNER FLASK, 100 MM CENTRE NECK, 2 X 45 MM SIDEARMS

4500-3L 1/pk
EUR 842.00
Description: Bioprocess Vessels; Spinner Flask 70

We discuss the trade-offs and practical design rules to follow to develop a microscope DH by the proposed solution. Using our prototype, we picture flowing marine microalgae, beads of polystyrene, E.coli and microplastics. We detail the effects on the performance and cost of each of the parameters, design and hardware selection, guiding the reader toward the realization of optimized device that can be used out of the lab by non-expert users for point of care testing.