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Validation of Image Quality and Diagnostic Accuracy Using a Mobile Phone Camera Microscope Adaptor Compared With Glass Slide Review in Teledermatopathology.
Ningbo
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new modalities to evaluate histopathology, such as imaging the entire slide, has been validated in the field of dermatopathology but often are not feasible and is not available in developing countries. Many are available worldwide, the mobile phone camera technology is a potentially simple and cheap method of imaging histological slides through the use of mobile camera microscope adapter.
This study aims to validate the use of an adapter that is available commercially in the diagnosis of inflammatory conditions and infections in dermatopathology. Representative images are taken from a slide to fifty-four cases by using an adapter and share through a cloud-based platform with five dermatopathologists given the diagnosis and assess the quality of the image.
After a washout period of 8 weeks, the same case assessed by dermatopathologists together using the original glass slide. Intraobserver level of concordance was 93.3%, and the image quality cell phone was rated as “excellent” or “diagnostic” in 94.4% of cases. This study validates the use of these low-tech and inexpensive adapter as a reliable tool in teledermatopathology. Study limitations include attached to an adapter and a limited panel of diagnosis. The main value of this device may be in developing countries, but the practicality and ease of use lends itself to use in academic settings and consultation in developed countries as well.
Natural History Museum, London (NHM) has embarked on an ambitious program to digitize its collection. One objective of this program is to improve workflow and infrastructure needed to support high-throughput digitizing and creating a comprehensive digital inventory of great scientific collections. This paper presents a workflow developed to digitize the entire Phthiraptera (parasitic lice) microscope slide collection (70 663 slides). Here we describe a new process of mass digitization semi-automatic use both temporary and permanent barcode label applied before and during imaging slide.
By using a series of barcode encoding the information associated with each slide (ie a unique identifier, location in the collection and taxonomic names), we can run a series of automated processes, including renaming files, image processing and bulk import into NHM collection management systems. We provide comparative data on the efficiency of this process, illustrates how simple activities, such as automatic file renaming, reducing image post-processing time, minimizing human error and can be applied in several types of collections.
HPV E6 / E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical lesions with Microscope Slides.
After breast and colon cancer, cervical cancer is the third most common cancer of women worldwide. Since the human papillomavirus (HPV) is known as the main cause of cervical cancer, HPV screening molecules when used together with cytology and histology method for the diagnosis of precancerous.
However, the HPV test’s sensitivity is currently less than 80%; thus, many cases of cervical cancer are not able to be diagnosed with HPV screening alone, and also, patients with cervical cancer are often determined to be HPV-negative with current screening methods.
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| PO101 | FD Neurotechnologies | 100 slides | EUR 186.41 |
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Description: Best quality products |
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) Gelatin-coated microscope slides (75 x 50 mm) |
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Description: Best quality products |
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) 100-place microscope slide box (for 75 x 25 mm slides) |
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Description: Best quality products |
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) 25-place microscope slide box (for 75 x 25 mm slides) |
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Description: Best quality products |
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Description: Best quality products |
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 Positive control tissue section for each antibody; Based on availability INQUIRE |
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 Proteinase |
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 ReadiUse™ microscope mounting solution |
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| 20009 | AAT Bioquest | 50 mL | EUR 115.00 |
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 Microscope Slide Boxes,100-Place, Blue |
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| LC7002-001 | GenDepot | Ea | EUR 70.00 |
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Microscope Slide Boxes,100-Place, Blue |
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| LC7002-010 | GenDepot | 10/PK | EUR 151.00 |
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Microscope Slide Boxes,100-Place, Blue |
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 Microscope Slide Boxes,100-Place, Green |
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Microscope Slide Boxes,100-Place, Green |
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 Microscope Slide Boxes,100-Place, Red |
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Microscope Slide Boxes,100-Place, Red |
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 Microscope Slide Boxes,100-Place, Yellow |
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Microscope Slide Boxes,100-Place, Yellow |
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Microscope Slide Boxes,100-Place, Yellow |
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 Microscope Slide Boxes,100-Place, White |
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Microscope Slide Boxes,100-Place, White |
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Microscope Slide Boxes,100-Place, White |
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Microscope Slide Boxes,25-Place, Blue |
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Microscope Slide Boxes,25-Place, Blue |
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 Microscope Slide Boxes,25-Place, Green |
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Microscope Slide Boxes,25-Place, Green |
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| LC7008-010 | GenDepot | 10/PK | EUR 124.00 |
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Microscope Slide Boxes,25-Place, Green |
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 Microscope Slide Boxes,25-Place, Red |
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Microscope Slide Boxes,25-Place, Red |
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Microscope Slide Boxes,25-Place, Red |
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 Microscope Slide Boxes,25-Place, Yellow |
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Microscope Slide Boxes,25-Place, Yellow |
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Microscope Slide Boxes,25-Place, Yellow |
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 Microscope Slide Boxes,25-Place, White |
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| LC7011-001 | GenDepot | EA | EUR 66.00 |
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Microscope Slide Boxes,25-Place, White |
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Microscope Slide Boxes,25-Place, White |
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| LC7011-100 | GenDepot | 100/CS | EUR 570.00 |
 Proteinase K |
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| AR0056 | BosterBio | 5mL | EUR 101.00 |
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Proteinase K |
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Proteinase K |
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Proteinase K |
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Proteinase K |
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Proteinase K |
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| GE0990-100MG | Glentham Life Sciences | 100 mg | EUR 94.00 |
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Proteinase K |
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| GE0990-1G | Glentham Life Sciences | 1 g | EUR 413.00 |
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Proteinase K |
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| GE0990-250MG | Glentham Life Sciences | 250 mg | EUR 154.00 |
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Proteinase K |
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| GE0990-25MG | Glentham Life Sciences | 25 mg | EUR 58.00 |
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Proteinase K |
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| GE0990-500MG | Glentham Life Sciences | 500 mg | EUR 249.00 |
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Proteinase K |
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| G029 | ABM | 1.0 ml | EUR 142.00 |
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Proteinase K |
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| K1037-1 | ApexBio | 1 ml | EUR 67.00 |
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Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease. |
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Proteinase K |
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| K1037-5 | ApexBio | 5 ml | EUR 108.00 |
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Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease. |
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Proteinase K |
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| P2180-010 | GenDepot | 100mg | EUR 182.00 |
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Proteinase K |
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| P2180-050 | GenDepot | 500mg | EUR 374.00 |
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Proteinase K |
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| P2180-100 | GenDepot | 1g | EUR 523.00 |
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Proteinase K |
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| Z5050002 | Biochain | 100 mg | EUR 171.00 |
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Description: No genetic downstream application can do without the good old Proteinase K. |
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) Amyloid (Control Slides) |
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| TCS0003-100 | ScyTek Laboratories | 100 Slides | EUR 706.00 |
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Amyloid (Control Slides) |
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| TCS0003-25 | ScyTek Laboratories | 25 Slides | EUR 232.00 |
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Amyloid (Control Slides) |
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| TCS0003-5 | ScyTek Laboratories | 5 Slides | EUR 98.00 |
) Argentaffin (Control Slides) |
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| TCS0004-100 | ScyTek Laboratories | 100 Slides | EUR 706.00 |
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Argentaffin (Control Slides) |
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| TCS0004-25 | ScyTek Laboratories | 25 Slides | EUR 232.00 |
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Argentaffin (Control Slides) |
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| TCS0004-5 | ScyTek Laboratories | 5 Slides | EUR 98.00 |
) Fungus (Control Slides) |
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| TCS0005-100 | ScyTek Laboratories | 100 Slides | EUR 706.00 |
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Fungus (Control Slides) |
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| TCS0005-25 | ScyTek Laboratories | 25 Slides | EUR 232.00 |
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Fungus (Control Slides) |
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| TCS0006-100 | ScyTek Laboratories | 100 Slides | EUR 706.00 |
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Bielschowsky's (Control Slides) |
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Bielschowsky's (Control Slides) |
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) Calcium (Control Slides) |
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| TCS0007-100 | ScyTek Laboratories | 100 Slides | EUR 706.00 |
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Calcium (Control Slides) |
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Calcium (Control Slides) |
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) Elastic (Control Slides) |
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| TCS0008-100 | ScyTek Laboratories | 100 Slides | EUR 706.00 |
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Elastic (Control Slides) |
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Elastic (Control Slides) |
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) Melanin (Control Slides) |
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| TCS0015-100 | ScyTek Laboratories | 100 Slides | EUR 706.00 |
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Melanin (Control Slides) |
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Melanin (Control Slides) |
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) Mucin (Control Slides) |
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| TCS0016-100 | ScyTek Laboratories | 100 Slides | EUR 706.00 |
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Mucin (Control Slides) |
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| TCS0016-25 | ScyTek Laboratories | 25 Slides | EUR 232.00 |
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Mucin (Control Slides) |
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| TCS0016-5 | ScyTek Laboratories | 5 Slides | EUR 98.00 |
) Reticulum (Control Slides) |
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| TCS0019-100 | ScyTek Laboratories | 100 Slides | EUR 706.00 |
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Reticulum (Control Slides) |
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| TCS0019-25 | ScyTek Laboratories | 25 Slides | EUR 232.00 |
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Reticulum (Control Slides) |
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| TCS0019-5 | ScyTek Laboratories | 5 Slides | EUR 98.00 |
 CometAssay High Throughput Slides |
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| 4252-02K-01 | Biotechne | 100 tests | EUR 1619.40 |
 CometAssay Alkaline Control Slides |
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| 4256-010-CC | Biotechne | 10 Tests | EUR 324.40 |
 CometAssay Neutral Control Slides |
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| 4257-010-NC | Biotechne | 10 Tests | EUR 324.40 |
) Alcian Blue (Control Slides) |
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| TCS0002-100 | ScyTek Laboratories | 100 Slides | EUR 706.00 |
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Alcian Blue (Control Slides) |
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| TCS0002-25 | ScyTek Laboratories | 25 Slides | EUR 232.00 |
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Alcian Blue (Control Slides) |
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| TCS0002-5 | ScyTek Laboratories | 5 Slides | EUR 98.00 |
) Helicobacter Pylori (Control Slides) |
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| TCS0011-100 | ScyTek Laboratories | 100 Slides | EUR 706.00 |
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Helicobacter Pylori (Control Slides) |
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| TCS0011-25 | ScyTek Laboratories | 25 Slides | EUR 232.00 |
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Helicobacter Pylori (Control Slides) |
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| TCS0011-5 | ScyTek Laboratories | 5 Slides | EUR 98.00 |
) Iron - Liver (Control Slides) |
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| TCS0013-100 | ScyTek Laboratories | 100 Slides | EUR 706.00 |
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Iron - Liver (Control Slides) |
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| TCS0013-25 | ScyTek Laboratories | 25 Slides | EUR 232.00 |
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Iron - Liver (Control Slides) |
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| TCS0013-5 | ScyTek Laboratories | 5 Slides | EUR 98.00 |
) Mast Cell (Control Slides) |
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| TCS0014-100 | ScyTek Laboratories | 100 Slides | EUR 706.00 |
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Mast Cell (Control Slides) |
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| TCS0014-25 | ScyTek Laboratories | 25 Slides | EUR 232.00 |
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Mast Cell (Control Slides) |
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| TCS0014-5 | ScyTek Laboratories | 5 Slides | EUR 98.00 |
) PAS/Glycogen (Control Slides) |
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| TCS0017-100 | ScyTek Laboratories | 100 Slides | EUR 706.00 |
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PAS/Glycogen (Control Slides) |
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| TCS0017-25 | ScyTek Laboratories | 25 Slides | EUR 232.00 |
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PAS/Glycogen (Control Slides) |
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| TCS0017-5 | ScyTek Laboratories | 5 Slides | EUR 98.00 |
) Trichrome - Colon (Control Slides) |
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| TCS0021-100 | ScyTek Laboratories | 100 Slides | EUR 706.00 |
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Trichrome - Colon (Control Slides) |
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| TCS0021-25 | ScyTek Laboratories | 25 Slides | EUR 232.00 |
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Trichrome - Colon (Control Slides) |
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| TCS0021-5 | ScyTek Laboratories | 5 Slides | EUR 98.00 |
) Trichrome - Liver (Control Slides) |
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| TCS0022-100 | ScyTek Laboratories | 100 Slides | EUR 706.00 |
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Trichrome - Liver (Control Slides) |
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| TCS0022-25 | ScyTek Laboratories | 25 Slides | EUR 232.00 |
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Trichrome - Liver (Control Slides) |
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| TCS0022-5 | ScyTek Laboratories | 5 Slides | EUR 98.00 |
 TOMO Slides 1 Box |
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| TOMO100 | ScyTek Laboratories | 100 Slides | EUR 90.00 |
 proteinase 3 Antibody |
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| abx430941-200ul | Abbexa | 200 ul | EUR 384.00 |
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 Proteinase K Antibody |
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| 6210-100 | Biovision | EUR 294.00 | |
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Proteinase K Antibody |
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| 6210-30T | Biovision | EUR 146.00 | |
 Human Proteinase-3 |
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| 7-03490 | CHI Scientific | 2µg | Ask for price |
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Human Proteinase-3 |
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| 7-03491 | CHI Scientific | 10µg | Ask for price |
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Human Proteinase-3 |
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| 7-03492 | CHI Scientific | 1mg | Ask for price |
 PROTEINASE K SOLUTION |
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| 501-PK | Viagen | 5 ml | EUR 152.00 |
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Description: Genomic PCR Grade Proteinase K Solution |
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 Proteinase 3 antibody |
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| 10R-1818 | Fitzgerald | 100 ul | EUR 403.00 |
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Description: Mouse monoclonal Proteinase 3 antibody |
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 Proteinase K protein |
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| 30R-2901 | Fitzgerald | 500 mg | EUR 425.00 |
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Description: Purified recombinant Human Proteinase K protein |
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 Proteinase 3 protein |
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| 30-1246 | Fitzgerald | 100 ug | EUR 543.00 |
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Description: Purified native Human Proteinase 3 protein |
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Proteinase 3 protein |
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| 30-AP70 | Fitzgerald | 200 ug | EUR 624.00 |
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Description: Purified native Human Proteinase 3 protein |
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Proteinase 3 antibody |
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| 20R-PR037 | Fitzgerald | 250 ul | EUR 457.00 |
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Description: Rabbit polyclonal Proteinase 3 antibody |
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Proteinase 3 protein |
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| 30R-AP027 | Fitzgerald | 100 ug | EUR 727.00 |
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Description: Purified native Human Proteinase 3 protein |
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 Proteinase 3 Antibody |
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| abx020707-1mg | Abbexa | 1 mg | EUR 801.00 |
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Proteinase 3 Antibody |
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| abx011403-100ul | Abbexa | 100 ul | EUR 411.00 |
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 Proteinase-3 Enzyme |
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| 20-abx073696 | Abbexa |
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Proteinase 3 Antibody |
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| BF0163 | Affbiotech | 200ul | EUR 376.00 |
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Description: Proteinase 3 antibody detects endogenous levels of total Proteinase 3. |
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 Proteinase K Solution |
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| BIO-37084 | Bioline | 5ml @ 20mg/ml | Ask for price |
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Proteinase K Solution |
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| BIO-37085 | Bioline | 5 x 5ml @ 20mg/ml | Ask for price |
 Proteinase K - 1g |
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| PB0451 | BBI Biotech | 1g | EUR 556.34 |
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 Proteinase K solution |
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| GZ5904-100ML | Glentham Life Sciences | 100 ml | EUR 907.00 |
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Proteinase K solution |
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| GZ5904-25ML | Glentham Life Sciences | 25 ml | EUR 349.00 |
 10x Proteinase K |
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| TG4039 | TopoGen | 0.5ml | EUR 134.00 |
) Solid Block (for slides / machining) |
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| BSW01 | BenchMark | 1 PC | EUR 182.23 |
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Solid Block (for slides / machining) |
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| BCM1412-BSW01 | BBI Biotech | 1 pcs, 1 UNIT | EUR 194.34 |
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 PROTEINASE K SOLUTION 10ML |
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| 502-PK | Viagen | 10 ml | EUR 239.00 |
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Description: Genomic PCR Grade Proteinase K Solution |
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 PROTEINASE K SOLUTION 25ML |
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| 503-PK | Viagen | 25 ml | EUR 502.00 |
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Description: Genomic PCR Grade Proteinase K Solution |
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 Proteinase 3 Antigen Protein |
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| abx061014-100ug | Abbexa | 100 ug | EUR 815.00 |
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 Antibody) Proteinase 3 (PR3) Antibody |
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| 20-abx100860 | Abbexa |
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Proteinase 3 (PR3) Antibody |
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| 20-abx100861 | Abbexa |
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Proteinase 3 (PR3) Antibody |
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| 20-abx100862 | Abbexa |
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Proteinase 3 (PR3) Antibody |
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| 20-abx178187 | Abbexa |
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Proteinase 3 (PR3) Antibody |
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| 20-abx174295 | Abbexa |
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Proteinase 3 (PR3) Antibody |
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| 20-abx174296 | Abbexa |
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 Proteinase 3 Blocking Peptide |
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| BF0163-BP | Affbiotech | 1mg | EUR 195.00 |
 Proteinase 3, Human Neutrophil |
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| PRT35-N-100 | Alpha Diagnostics | 25 ug | EUR 225.00 |
 PRTN3 Proteinase-3 Human |
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| PROTP24158 | BosterBio | Regular: 10ug | EUR 317.00 |
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Description: PRTN3 is a natural antigen having a molecular mass of 25kDa to 32kDa due to glycosylation isoforms. PRTN3 is isolated from human peripheral blood leukocytes. |
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 Proteinase K, 20mg/ml |
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| P2170-002 | GenDepot | 2X1ml | EUR 151.00 |
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Proteinase K, 20mg/ml |
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| P2170-005 | GenDepot | 5X1ml | EUR 199.00 |
) anti-Proteinase 3 (3B4) |
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| LF-MA30307 | Abfrontier | 100 ul | EUR 486.00 |
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Description: Mouse Monoclonal to Proteinase 3 |
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) Recombinant Proteinase 3 (PR3) |
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| 4-RPB434Hu01 | Cloud-Clone |
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Description: Recombinant Human Proteinase 3 expressed in: E.coli |
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Recombinant Proteinase 3 (PR3) |
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| 4-RPB434Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Proteinase 3 expressed in: E.coli |
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Recombinant Proteinase 3 (PR3) |
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| 4-RPB434Ra01 | Cloud-Clone |
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Description: Recombinant Rat Proteinase 3 expressed in: E.coli |
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) MEF Feeder Cells (Hygromycin-resistant) |
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| CBA-313 | Cell Biolabs | 1 vial | EUR 461.00 |
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Description: MEF Feeder Cells are mouse embryonic fibroblasts that allow stem cell culture without LIF. Cells must be mitotically inactivated prior to addition of stem cells. Hygromycin resistant. |
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 Methicillin-Resistant Staphylococcus Aureus Antibody |
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| 20-abx137142 | Abbexa |
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) MEF Feeder Cells (Neomycin-resistant) |
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| CBA-311 | Cell Biolabs | 1 vial | EUR 461.00 |
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Description: MEF Feeder Cells are mouse embryonic fibroblasts that allow stem cell culture without LIF. Cells must be mitotically inactivated prior to addition of stem cells. Neomycin resistant. |
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) MEF Feeder Cells (Puromycin-resistant) |
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| CBA-312 | Cell Biolabs | 1 vial | EUR 461.00 |
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Description: MEF Feeder Cells are mouse embryonic fibroblasts that allow stem cell culture without LIF. Cells must be mitotically inactivated prior to addition of stem cells. Puromycin resistant. |
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 Vancomycin resistant enterococci PCR kit |
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| PCR-H487-48D | Bioingentech | 50T | EUR 425.80 |
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Description: An conventional PCR kit for detection of Vancomycin resistant enterococci |
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Vancomycin resistant enterococci PCR kit |
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| PCR-H487-96D | Bioingentech | 100T | EUR 521.50 |
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Description: An conventional PCR kit for detection of Vancomycin resistant enterococci |
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 Water-Resistant, Traceable Flashing Timer |
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| LE9004 | GenDepot | Ea | EUR 80.00 |
 anti-Tartrate Resistant Acid Phosphatase |
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| YF-PA10048 | Abfrontier | 50 ug | EUR 363.00 |
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Description: Mouse polyclonal to Tartrate Resistant Acid Phosphatase |
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anti-Tartrate Resistant Acid Phosphatase |
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| YF-PA10049 | Abfrontier | 100 ug | EUR 403.00 |
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Description: Rabbit polyclonal to Tartrate Resistant Acid Phosphatase |
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anti-Tartrate Resistant Acid Phosphatase |
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| YF-PA23162 | Abfrontier | 50 ul | EUR 334.00 |
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Description: Mouse polyclonal to Tartrate Resistant Acid Phosphatase |
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 Slide dryer attachment for 3 slides |
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| B1000-03 | BenchMark | 1 PC | EUR 197.97 |
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 Mouse EmbryoSections, fixed/paraffin, 10 slides |
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| PE102 | FD Neurotechnologies | each | Ask for price |
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Description: Best quality products |
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Therefore, the human telomerase reverse transcriptase (hTERT) and Ki67 previously identified as cancer markers tested. And cells exfoliated cervical high-grade squamous intraepithelial lesions (HSIL), the most severe precancerous lesions of cancer, which is used in this study. However, it takes a long time to collect enough specimens to perform statistical analysis.
