Validation of Image Quality and Diagnostic Accuracy Using a Mobile Phone Camera Microscope Adaptor Compared With Glass Slide Review in Teledermatopathology.

new modalities to evaluate histopathology, such as imaging the entire slide, has been validated in the field of dermatopathology but often are not feasible and is not available in developing countries. Many are available worldwide, the mobile phone camera technology is a potentially simple and cheap method of imaging histological slides through the use of mobile camera microscope adapter.
This study aims to validate the use of an adapter that is available commercially in the diagnosis of inflammatory conditions and infections in dermatopathology. Representative images are taken from a slide to fifty-four cases by using an adapter and share through a cloud-based platform with five dermatopathologists given the diagnosis and assess the quality of the image.
After a washout period of 8 weeks, the same case assessed by dermatopathologists together using the original glass slide. Intraobserver level of concordance was 93.3%, and the image quality cell phone was rated as “excellent” or “diagnostic” in 94.4% of cases. This study validates the use of these low-tech and inexpensive adapter as a reliable tool in teledermatopathology. Study limitations include attached to an adapter and a limited panel of diagnosis. The main value of this device may be in developing countries, but the practicality and ease of use lends itself to use in academic settings and consultation in developed countries as well.
Natural History Museum, London (NHM) has embarked on an ambitious program to digitize its collection. One objective of this program is to improve workflow and infrastructure needed to support high-throughput digitizing and creating a comprehensive digital inventory of great scientific collections. This paper presents a workflow developed to digitize the entire Phthiraptera (parasitic lice) microscope slide collection (70 663 slides). Here we describe a new process of mass digitization semi-automatic use both temporary and permanent barcode label applied before and during imaging slide.
By using a series of barcode encoding the information associated with each slide (ie a unique identifier, location in the collection and taxonomic names), we can run a series of automated processes, including renaming files, image processing and bulk import into NHM collection management systems. We provide comparative data on the efficiency of this process, illustrates how simple activities, such as automatic file renaming, reducing image post-processing time, minimizing human error and can be applied in several types of collections.

HPV E6 / E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical lesions with Microscope Slides.
After breast and colon cancer, cervical cancer is the third most common cancer of women worldwide. Since the human papillomavirus (HPV) is known as the main cause of cervical cancer, HPV screening molecules when used together with cytology and histology method for the diagnosis of precancerous.
However, the HPV test’s sensitivity is currently less than 80%; thus, many cases of cervical cancer are not able to be diagnosed with HPV screening alone, and also, patients with cervical cancer are often determined to be HPV-negative with current screening methods.
Microscope Slides Plain 1.0-1.2mm 76x52mm |
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MIC2156 | Scientific Laboratory Supplies | PK50 | EUR 15.73 |
Cardboard tray for 20 microscope slides |
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DD65205 | Scientific Laboratory Supplies | EACH | EUR 15.85 |
Box for 25 microscope slides purple colour |
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DD49719 | Scientific Laboratory Supplies | EACH | EUR 15.28 |
Box for 25 microscope slides orange colour |
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DD49720 | Scientific Laboratory Supplies | EACH | EUR 15.28 |
Box for 25 microscope slides turquoise colour |
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DD49721 | Scientific Laboratory Supplies | EACH | EUR 15.28 |
Gelatin-coated microscope slides (75 x 25 mm) |
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PO101 | FD Neurotechnologies | 100 slides | EUR 236.4 |
Gelatin-coated microscope slides (75 x 50 mm) |
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PO105 | FD Neurotechnologies | 100 slides | EUR 420 |
Polystyrene box for 100 microscope slides blue colour |
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DD139537 | Scientific Laboratory Supplies | EACH | EUR 10.49 |
Polystyrene box for 100 microscope slides green colour |
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DD139538 | Scientific Laboratory Supplies | EACH | EUR 10.49 |
Plastic box for 100 microscope slides (blue colour) |
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DD37501 | Scientific Laboratory Supplies | EACH | EUR 29.64 |
Plastic box for 100 microscope slides (red colour) |
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DD37503 | Scientific Laboratory Supplies | EACH | EUR 29.64 |
Plastic box for 50 microscope slides (blue colour) |
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DD37507 | Scientific Laboratory Supplies | EACH | EUR 25.08 |
Plastic box for 100 microscope slides (assorted colours) cork bottom |
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DD10197 | Scientific Laboratory Supplies | PK5 | EUR 83.22 |
Microscope Slides 26X76mm Ground Edges Double Frosted 45° Angle -Silver- |
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VBS656 | Scientific Laboratory Supplies | PK2500 | EUR 232.56 |
100-place microscope slide box (for 75 x 25 mm slides) |
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PO201 | FD Neurotechnologies | each | EUR 84 |
25-place microscope slide box (for 75 x 25 mm slides) |
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PO202 | FD Neurotechnologies | each | EUR 73.2 |
100-place microscope slide box (for 75 x 50 mm slides) |
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PO203 | FD Neurotechnologies | each | EUR 152.4 |
Microscope slide dispenser for box of 72 standard 25 x 75mm slides |
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M7100-SD | MTC Bio | 1/pack | EUR 83.89 |
Description: Microscope slide dispenser |
Microscope slides, positive charged, 45° corners, frosted on one end, 25 x 75mm |
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M7100-45 | MTC Bio | 72/pack | EUR 75.4 |
Description: Microscope slides positive charged |
Microscope slides, positive charged, 90° corners, frosted on one end, 25 x 75mm |
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M7100-90 | MTC Bio | 72/pack | EUR 75.4 |
Description: Microscope slides positive charged |
Stereo Microscope Trinocular |
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MIC1590 | Scientific Laboratory Supplies | EACH | EUR 1022.58 |
Positive control tissue section for each antibody; Based on availability INQUIRE |
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Control-Slides | Innovex | Set of 5 | EUR 211.2 |
Proteinase |
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HY-B2228 | MedChemExpress | 1g | EUR 142.8 |
BA310 Elite Trinocular microscope |
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MIC2937 | Scientific Laboratory Supplies | EACH | EUR 2139.78 |
Microscope Slide Polysine 75x25x1mm |
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MIC3050 | Scientific Laboratory Supplies | PK72 | EUR 34.66 |
BA210E Elite Binocular Microscope |
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MIC5633 | Scientific Laboratory Supplies | EACH | EUR 1451.22 |
Proteinase K |
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abx082041-100mg | Abbexa | 100 mg | EUR 309.6 |
Proteinase K |
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abx098140-1ml | Abbexa | 1 ml | EUR 226.8 |
Proteinase K |
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BIO-37037 | Bioline | 100mg | Ask for price |
Proteinase K |
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BIO-37039 | Bioline | 1000mg | Ask for price |
Proteinase K |
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AR0056 | BosterBio | 5mL | EUR 121.2 |
Proteinase K |
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G029 | ABM | 1.0 ml | EUR 170.4 |
Proteinase K |
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GE0990-100MG | Glentham Life Sciences | 100 mg | EUR 112.8 |
Proteinase K |
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GE0990-1G | Glentham Life Sciences | 1 g | EUR 495.6 |
Proteinase K |
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GE0990-250MG | Glentham Life Sciences | 250 mg | EUR 184.8 |
Proteinase K |
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GE0990-25MG | Glentham Life Sciences | 25 mg | EUR 69.6 |
Proteinase K |
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GE0990-500MG | Glentham Life Sciences | 500 mg | EUR 298.8 |
Proteinase K |
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K1037-1 | ApexBio | 1 ml | EUR 80.4 |
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease. |
Proteinase K |
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K1037-5 | ApexBio | 5 ml | EUR 129.6 |
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease. |
Proteinase K |
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P2180-010 | GenDepot | 100mg | EUR 218.4 |
Proteinase K |
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P2180-050 | GenDepot | 500mg | EUR 448.8 |
Proteinase K |
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P2180-100 | GenDepot | 1g | EUR 627.6 |
Proteinase K |
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Z5050002 | Biochain | 100 mg | EUR 205.2 |
Description: No genetic downstream application can do without the good old Proteinase K. |
Proteinase K |
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M-CO-404 | MiTeGen | 100 mg | EUR 376 |
Description: Proteinase K |
Premium Plain Glass Microscope Slides, 144 /pk, 1 mm thickness, 25 mm wide by 73 mm long |
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M-ACC-GSL9 | MiTeGen | 200 TIPS | EUR 65 |
Description: Premium Plain Glass Microscope Slides, 144 /pk, 1 mm thickness, 25 mm wide by 73 mm long |
ReadiUse™ microscope mounting solution |
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20009 | AAT Bioquest | 50 mL | EUR 138 |
Digi-Pad Microscope Tablet Camera |
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MIC0115 | Scientific Laboratory Supplies | EACH | EUR 1144.56 |
Motic BA210 LED Microscope Binocular |
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MIC2004 | Scientific Laboratory Supplies | EACH | EUR 1337.22 |
Motic BA310 LED Microscope Binocular |
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MIC2016 | Scientific Laboratory Supplies | EACH | EUR 1805.76 |
Motic BA310 LED Microscope Trinocular |
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MIC2018 | Scientific Laboratory Supplies | EACH | EUR 2042.88 |
Motic SMZ-168-BL Microscope |
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MIC2800 | Scientific Laboratory Supplies | EACH | EUR 1357.74 |
Microscope Slide Box True North |
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MIC4090 | Scientific Laboratory Supplies | EACH | EUR 38.19 |
Amyloid (Control Slides) |
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TCS0003-100 | ScyTek Laboratories | 100 Slides | EUR 847.2 |
Amyloid (Control Slides) |
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TCS0003-25 | ScyTek Laboratories | 25 Slides | EUR 278.4 |
Amyloid (Control Slides) |
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TCS0003-5 | ScyTek Laboratories | 5 Slides | EUR 117.6 |
Argentaffin (Control Slides) |
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TCS0004-100 | ScyTek Laboratories | 100 Slides | EUR 847.2 |
Argentaffin (Control Slides) |
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TCS0004-25 | ScyTek Laboratories | 25 Slides | EUR 278.4 |
Argentaffin (Control Slides) |
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TCS0004-5 | ScyTek Laboratories | 5 Slides | EUR 117.6 |
Fungus (Control Slides) |
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TCS0005-100 | ScyTek Laboratories | 100 Slides | EUR 847.2 |
Fungus (Control Slides) |
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TCS0005-25 | ScyTek Laboratories | 25 Slides | EUR 278.4 |
Fungus (Control Slides) |
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TCS0005-5 | ScyTek Laboratories | 5 Slides | EUR 117.6 |
Bielschowsky's (Control Slides) |
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TCS0006-100 | ScyTek Laboratories | 100 Slides | EUR 847.2 |
Bielschowsky's (Control Slides) |
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TCS0006-25 | ScyTek Laboratories | 25 Slides | EUR 278.4 |
Bielschowsky's (Control Slides) |
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TCS0006-5 | ScyTek Laboratories | 5 Slides | EUR 117.6 |
Calcium (Control Slides) |
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TCS0007-100 | ScyTek Laboratories | 100 Slides | EUR 847.2 |
Calcium (Control Slides) |
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TCS0007-25 | ScyTek Laboratories | 25 Slides | EUR 278.4 |
Calcium (Control Slides) |
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TCS0007-5 | ScyTek Laboratories | 5 Slides | EUR 117.6 |
Elastic (Control Slides) |
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TCS0008-100 | ScyTek Laboratories | 100 Slides | EUR 847.2 |
Elastic (Control Slides) |
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TCS0008-25 | ScyTek Laboratories | 25 Slides | EUR 278.4 |
Elastic (Control Slides) |
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TCS0008-5 | ScyTek Laboratories | 5 Slides | EUR 117.6 |
Melanin (Control Slides) |
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TCS0015-100 | ScyTek Laboratories | 100 Slides | EUR 847.2 |
Melanin (Control Slides) |
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TCS0015-25 | ScyTek Laboratories | 25 Slides | EUR 278.4 |
Melanin (Control Slides) |
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TCS0015-5 | ScyTek Laboratories | 5 Slides | EUR 117.6 |
Mucin (Control Slides) |
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TCS0016-100 | ScyTek Laboratories | 100 Slides | EUR 847.2 |
Mucin (Control Slides) |
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TCS0016-25 | ScyTek Laboratories | 25 Slides | EUR 278.4 |
Mucin (Control Slides) |
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TCS0016-5 | ScyTek Laboratories | 5 Slides | EUR 117.6 |
Reticulum (Control Slides) |
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TCS0019-100 | ScyTek Laboratories | 100 Slides | EUR 847.2 |
Reticulum (Control Slides) |
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TCS0019-25 | ScyTek Laboratories | 25 Slides | EUR 278.4 |
Reticulum (Control Slides) |
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TCS0019-5 | ScyTek Laboratories | 5 Slides | EUR 117.6 |
CO2 Resistant Shaker |
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SHA1004 | Scientific Laboratory Supplies | EACH | EUR 4081.2 |
Proteinase K Antibody |
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6210-100 | Biovision | EUR 352.8 |
Proteinase K Antibody |
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6210-30T | Biovision | EUR 175.2 |
PROTEINASE K SOLUTION |
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501-PK | Viagen | 5 ml | EUR 182.4 |
Description: Genomic PCR Grade Proteinase K Solution |
Human Proteinase-3 |
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7-03490 | CHI Scientific | 2µg | Ask for price |
Human Proteinase-3 |
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7-03491 | CHI Scientific | 10µg | Ask for price |
Human Proteinase-3 |
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7-03492 | CHI Scientific | 1mg | Ask for price |
Proteinase K protein |
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30R-2901 | Fitzgerald | 500 mg | EUR 510 |
Description: Purified recombinant Human Proteinase K protein |
Proteinase 3 antibody |
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20R-PR037 | Fitzgerald | 250 ul | EUR 548.4 |
Description: Rabbit polyclonal Proteinase 3 antibody |
Proteinase 3 protein |
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30R-AP027 | Fitzgerald | 100 ug | EUR 872.4 |
Description: Purified native Human Proteinase 3 protein |
Proteinase 3 antibody |
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10R-1818 | Fitzgerald | 100 ul | EUR 483.6 |
Description: Mouse monoclonal Proteinase 3 antibody |
Proteinase 3 protein |
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30-1246 | Fitzgerald | 100 ug | EUR 651.6 |
Description: Purified native Human Proteinase 3 protein |
Proteinase 3 protein |
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30-AP70 | Fitzgerald | 200 ug | EUR 748.8 |
Description: Purified native Human Proteinase 3 protein |
Proteinase-3 Enzyme |
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20-abx073696 | Abbexa |
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Proteinase 3 Antibody |
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abx011403-100ul | Abbexa | 100 ul | EUR 493.2 |
Proteinase 3 Antibody |
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abx020707-1mg | Abbexa | 1 mg | EUR 961.2 |
proteinase 3 Antibody |
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abx430941-200ul | Abbexa | 200 ul | EUR 460.8 |
Proteinase 3 Antibody |
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BF0163 | Affbiotech | 200ul | EUR 540 |
Proteinase K Solution |
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BIO-37084 | Bioline | 5ml @ 20mg/ml | Ask for price |
Proteinase K Solution |
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BIO-37085 | Bioline | 5 x 5ml @ 20mg/ml | Ask for price |
Proteinase K solution |
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GZ5904-100ML | Glentham Life Sciences | 100 ml | EUR 1088.4 |
Proteinase K solution |
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GZ5904-25ML | Glentham Life Sciences | 25 ml | EUR 418.8 |
Proteinase K - 1g |
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PB0451 | Bio Basic | 1g | EUR 667.61 |
10x Proteinase K |
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TG4039 | TopoGen | 0.5ml | EUR 160.8 |
Microscope Slide Boxes,100-Place, Blue |
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LC7002-001 | GenDepot | Ea | EUR 84 |
Microscope Slide Boxes,100-Place, Blue |
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LC7002-010 | GenDepot | 10/PK | EUR 181.2 |
Microscope Slide Boxes,100-Place, Blue |
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LC7002-100 | GenDepot | 100/CS | EUR 967.2 |
Microscope Slide Boxes,100-Place, Green |
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LC7003-001 | GenDepot | Ea | EUR 84 |
Microscope Slide Boxes,100-Place, Green |
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LC7003-010 | GenDepot | 10/PK | EUR 181.2 |
Microscope Slide Boxes,100-Place, Green |
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LC7003-100 | GenDepot | 100/CS | EUR 967.2 |
Microscope Slide Boxes,100-Place, Red |
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LC7004-001 | GenDepot | Ea | EUR 84 |
Microscope Slide Boxes,100-Place, Red |
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LC7004-010 | GenDepot | 10/PK | EUR 181.2 |
Microscope Slide Boxes,100-Place, Red |
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LC7004-100 | GenDepot | 100/CS | EUR 967.2 |
Microscope Slide Boxes,100-Place, Yellow |
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LC7005-001 | GenDepot | Ea | EUR 84 |
Microscope Slide Boxes,100-Place, Yellow |
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LC7005-010 | GenDepot | 10/PK | EUR 181.2 |
Microscope Slide Boxes,100-Place, Yellow |
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LC7005-100 | GenDepot | 100/CS | EUR 967.2 |
Microscope Slide Boxes,100-Place, White |
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LC7006-001 | GenDepot | Ea | EUR 84 |
Microscope Slide Boxes,100-Place, White |
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LC7006-010 | GenDepot | 10/PK | EUR 181.2 |
Microscope Slide Boxes,100-Place, White |
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LC7006-100 | GenDepot | 100/CS | EUR 967.2 |
Microscope Slide Boxes,25-Place, Blue |
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LC7007-001 | GenDepot | EA | EUR 79.2 |
Microscope Slide Boxes,25-Place, Blue |
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LC7007-010 | GenDepot | 10/PK | EUR 148.8 |
Microscope Slide Boxes,25-Place, Blue |
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LC7007-100 | GenDepot | 100/CS | EUR 684 |
Microscope Slide Boxes,25-Place, Green |
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LC7008-001 | GenDepot | EA | EUR 79.2 |
Microscope Slide Boxes,25-Place, Green |
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LC7008-010 | GenDepot | 10/PK | EUR 148.8 |
Microscope Slide Boxes,25-Place, Green |
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LC7008-100 | GenDepot | 100/CS | EUR 684 |
Microscope Slide Boxes,25-Place, Red |
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LC7009-001 | GenDepot | EA | EUR 79.2 |
Microscope Slide Boxes,25-Place, Red |
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LC7009-010 | GenDepot | 10/PK | EUR 148.8 |
Microscope Slide Boxes,25-Place, Red |
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LC7009-100 | GenDepot | 100/CS | EUR 684 |
Microscope Slide Boxes,25-Place, Yellow |
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LC7010-001 | GenDepot | EA | EUR 79.2 |
Microscope Slide Boxes,25-Place, Yellow |
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LC7010-010 | GenDepot | 10/PK | EUR 148.8 |
Microscope Slide Boxes,25-Place, Yellow |
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LC7010-100 | GenDepot | 100/CS | EUR 684 |
Microscope Slide Boxes,25-Place, White |
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LC7011-001 | GenDepot | EA | EUR 79.2 |
Microscope Slide Boxes,25-Place, White |
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LC7011-010 | GenDepot | 10/PK | EUR 148.8 |
Microscope Slide Boxes,25-Place, White |
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LC7011-100 | GenDepot | 100/CS | EUR 684 |
VIKEM(R) VINYL MICROSCOPE COVER EXTRA |
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BAF243040000-1EA | Scientific Laboratory Supplies | EACH | EUR 32.38 |
Microscope Binoc MAGNUM-B planachrom obj |
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MIC0105 | Scientific Laboratory Supplies | EACH | EUR 1800.06 |
Microscope Trinoc MAGNUM-T planachrom obj |
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MIC0107 | Scientific Laboratory Supplies | EACH | EUR 1868.46 |
Microscope Pack 1 CX23 100x Oil |
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MIC1968 | Scientific Laboratory Supplies | EACH | EUR 1827.42 |
Microscope Pack 2 CX23 BIN LED |
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MIC1970 | Scientific Laboratory Supplies | EACH | EUR 1447.8 |
Microscope Cover Slips No. 0 18x18mm |
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MIC2158 | Scientific Laboratory Supplies | PK100 | EUR 2.28 |
Microscope Cover Slips No. 0 22x22mm |
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MIC2160 | Scientific Laboratory Supplies | PK100 | EUR 2.96 |
Microscope Cover Slips No. 1 18x18mm |
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MIC2162 | Scientific Laboratory Supplies | PK100 | EUR 1.94 |
Microscope Cover Slips No. 1 22x22mm |
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MIC2164 | Scientific Laboratory Supplies | PK100 | EUR 2.62 |
Microscope Cover Slips No. 1.5 18x18mm |
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MIC2166 | Scientific Laboratory Supplies | PK100 | EUR 2.05 |
Microscope Cover Slips No. 1.5 22x22mm |
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MIC2168 | Scientific Laboratory Supplies | PK100 | EUR 2.96 |
Microscope Cover Slips No.1.5 22x26mm |
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MIC2170 | Scientific Laboratory Supplies | PK100 | EUR 3.53 |
Microscope Cover Slips No. 1.5 22x32mm |
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MIC2172 | Scientific Laboratory Supplies | PK100 | EUR 4.1 |
Microscope Cover Slips No. 1.5 22x40mm |
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MIC2174 | Scientific Laboratory Supplies | PK100 | EUR 4.9 |
Microscope Cover Slips No. 2 18x18mm |
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MIC2176 | Scientific Laboratory Supplies | PK100 | EUR 2.39 |
Microscope Cover Slips No. 2 22x22mm |
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MIC2178 | Scientific Laboratory Supplies | PK100 | EUR 3.31 |
Microscope Cover Slips No. 1.5 16mm |
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MIC2182 | Scientific Laboratory Supplies | PK100 | EUR 8.89 |
Microscope Slide Box 100 Place Tall |
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MIC4080 | Scientific Laboratory Supplies | EACH | EUR 38.19 |
Microscope Slide Box 25 ULT safe |
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MIC4092 | Scientific Laboratory Supplies | EACH | EUR 17.62 |
SMZ-171-Binocular LED Stero Microscope |
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MIC5444 | Scientific Laboratory Supplies | EACH | EUR 1596 |
SMZ-171-Trinocular LED Stero Microscope |
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MIC5445 | Scientific Laboratory Supplies | EACH | EUR 1691.76 |
SMZ-171-Binocular P Stero Microscope |
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MIC5446 | Scientific Laboratory Supplies | EACH | EUR 1244.88 |
SMZ-171-Trinocular P Stero Microscope |
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MIC5447 | Scientific Laboratory Supplies | EACH | EUR 1338.36 |
Motic SMZ-140-N2GG Stereo Microscope |
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MIC5900 | Scientific Laboratory Supplies | EACH | EUR 621.3 |
Motic SMZ-143-N2GG Stereo Microscope |
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MIC5902 | Scientific Laboratory Supplies | EACH | EUR 722.76 |
BRAND(TM) MICROSCOPE SLIDE CUT EDGES |
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BR474701-2500EA | Scientific Laboratory Supplies | PK2500 | EUR 175.67 |
MIGHTY SCOPE(TM) DIGITAL MICROSCOPE 5M |
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Z742668-1EA | Scientific Laboratory Supplies | EACH | EUR 348.04 |
CometAssay High Throughput Slides |
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4252-02K-01 | Biotechne | 100 tests | EUR 1943.28 |
CometAssay Alkaline Control Slides |
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4256-010-CC | Biotechne | 10 Tests | EUR 389.28 |
Therefore, the human telomerase reverse transcriptase (hTERT) and Ki67 previously identified as cancer markers tested. And cells exfoliated cervical high-grade squamous intraepithelial lesions (HSIL), the most severe precancerous lesions of cancer, which is used in this study. However, it takes a long time to collect enough specimens to perform statistical analysis.