A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.

A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.

In this paper we report the development of a modular, cost-effective open source, and fully automated slide-scanning microscope, composed entirely of readily available parts of the off-the-shelf, and capable of bright field and fluorescence mode.

Automatic X-Y stage consists of two steps Cheap micrometer coupled with the stepper motor is operated in open loop mode. The microscope consists of low cost CMOS sensor and lens’s board placed in the 4f configuration. This system has a resolution of about 1 micron, is limited by the f / # lens boards are available. The microscope is compact, measuring just 25 × 25 × 30 cm, and have the absolute positioning accuracy of ± 1 m in the X and the direction Y. A Z-phase imaging allows autofocus and a wider field of view even on non-planar samples, and custom software enables automatic determination of the boundaries of the sample and image mosaicking.

We demonstrate the utility of our devices through the blood imaging fluorescent- and transmission-dye stained and smears feces containing parasite of humans and animals, as well as some tissue samples ready. These results indicate that the image quality comparable to high-end commercial microscopes with a cost of less than US $ 400 for the bright-field system, with an additional US $ 100 is required for the fluorescence module.


In the current trend of miniaturization and simplification of imaging flow cytometry, Lab-on-a-Chip (LOC) of the microfluidic device represents an innovative and cost-effective solutions. Within this framework, we propose for the first time the platform novel based on the compactness of the microscope slide holographic (HMS) in combination with computing features the new space-time holography digital (STDH) using a 1D linear sensor array (LSA) is not a 2D CCD or CMOS camera to respond real diagnostic needs. In this LoC platform, computational methods, holography, and microfluidics interlinked to provide an imaging system by reducing the number of optical components and the ability to achieve a reliable cell counting even in the absence of highly accurate flow control. STDH utilize motion sample into a microfluidic channel to get the field-of-view is limited along the direction of flow, independent of magnification factor.

Furthermore, the typical numerical refocusing from modality hologram allows imaging and visualization of the entire volume of the channel, thus avoiding the loss of information due to the limited depth of focus of a standard microscope. As a result, we believe that this platform could open new perspectives to improve throughput by 3D volumetric imaging.

 A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.
A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.

Droplet spray ionization of a glass microscope slide: real-time monitoring of polymerization of ethylene.

Ambient ionization mass spectrometry is achieved in a simple manner by loading a sample solution onto a glass microscope cover corner is positioned in front of the inlet to the mass spectrometer and applying a high voltage to the sample. The resulting flow of charged droplets is stable, has no contamination from the substrate platform, and can be used repeatedly.

Gelatin-coated microscope slides (75 x 50 mm)

PO105 100 slides
EUR 334.25
Description: Best quality products

DiagNano Fluorophore Labeled Gold Nanoparticles, 90 nm

GFL-90 1 mL
EUR 1053

100-place microscope slide box (for 75 x 25 mm slides)

PO201 each
EUR 67.03
Description: Best quality products

25-place microscope slide box (for 75 x 25 mm slides)

PO202 each
EUR 59.1
Description: Best quality products

100-place microscope slide box (for 75 x 50 mm slides)

PO203 each
EUR 114.36
Description: Best quality products

Microscope slide dispenser for box of 72 standard 25 x 75mm slides

M7100-SD 1/pack
EUR 69.91
Description: Microscope slide dispenser

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 90 nm

GCK-M-90 1 kit
EUR 757

Custom Peptide Synthesis (>90%; mass spec, hplc (mg-kg size, price based upon peptide size: 2-100 aa)

PEP-90 1 Ask for price

Microscope slides, positive charged, 45° corners, frosted on one end, 25 x 75mm

M7100-45 72/pack
EUR 62.83
Description: Microscope slides positive charged

HEV-IgM Hepatitis E IgM ELISA test

90 96T/Box Ask for price
  • Area of application: Hepatitis testing
Description: ELISA based test for quantitative detection of HEV-IgM Hepatitis E IgM

Positive control tissue section for each antibody; Based on availability INQUIRE

Control-Slides Set of 5
EUR 176

ReadiUse™ microscope mounting solution

20009 50 mL
EUR 115
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Amyloid (Control Slides)

TCS0003-100 100 Slides
EUR 706

Amyloid (Control Slides)

TCS0003-25 25 Slides
EUR 232

Amyloid (Control Slides)

TCS0003-5 5 Slides
EUR 98

Argentaffin (Control Slides)

TCS0004-100 100 Slides
EUR 706

Argentaffin (Control Slides)

TCS0004-25 25 Slides
EUR 232

Argentaffin (Control Slides)

TCS0004-5 5 Slides
EUR 98

Fungus (Control Slides)

TCS0005-100 100 Slides
EUR 706

Fungus (Control Slides)

TCS0005-25 25 Slides
EUR 232

Fungus (Control Slides)

TCS0005-5 5 Slides
EUR 98

Bielschowsky's (Control Slides)

TCS0006-100 100 Slides
EUR 706

Bielschowsky's (Control Slides)

TCS0006-25 25 Slides
EUR 232

Bielschowsky's (Control Slides)

TCS0006-5 5 Slides
EUR 98

Calcium (Control Slides)

TCS0007-100 100 Slides
EUR 706

Calcium (Control Slides)

TCS0007-25 25 Slides
EUR 232

Calcium (Control Slides)

TCS0007-5 5 Slides
EUR 98

Elastic (Control Slides)

TCS0008-100 100 Slides
EUR 706

Elastic (Control Slides)

TCS0008-25 25 Slides
EUR 232

Elastic (Control Slides)

TCS0008-5 5 Slides
EUR 98

Melanin (Control Slides)

TCS0015-100 100 Slides
EUR 706

Melanin (Control Slides)

TCS0015-25 25 Slides
EUR 232

Melanin (Control Slides)

TCS0015-5 5 Slides
EUR 98

Mucin (Control Slides)

TCS0016-100 100 Slides
EUR 706

Mucin (Control Slides)

TCS0016-25 25 Slides
EUR 232

Mucin (Control Slides)

TCS0016-5 5 Slides
EUR 98

Reticulum (Control Slides)

TCS0019-100 100 Slides
EUR 706

Reticulum (Control Slides)

TCS0019-25 25 Slides
EUR 232

Reticulum (Control Slides)

TCS0019-5 5 Slides
EUR 98

Microscope Slide Boxes,100-Place, Blue

LC7002-001 Ea
EUR 70

Microscope Slide Boxes,100-Place, Blue

LC7002-010 10/PK
EUR 151

Microscope Slide Boxes,100-Place, Blue

LC7002-100 100/CS
EUR 806

Microscope Slide Boxes,100-Place, Green

LC7003-001 Ea
EUR 70

Microscope Slide Boxes,100-Place, Green

LC7003-010 10/PK
EUR 151

Microscope Slide Boxes,100-Place, Green

LC7003-100 100/CS
EUR 806

Microscope Slide Boxes,100-Place, Red

LC7004-001 Ea
EUR 70

Microscope Slide Boxes,100-Place, Red

LC7004-010 10/PK
EUR 151

Microscope Slide Boxes,100-Place, Red

LC7004-100 100/CS
EUR 806

Microscope Slide Boxes,100-Place, Yellow

LC7005-001 Ea
EUR 70

Microscope Slide Boxes,100-Place, Yellow

LC7005-010 10/PK
EUR 151

Microscope Slide Boxes,100-Place, Yellow

LC7005-100 100/CS
EUR 806

Microscope Slide Boxes,100-Place, White

LC7006-001 Ea
EUR 70

Microscope Slide Boxes,100-Place, White

LC7006-010 10/PK
EUR 151

Microscope Slide Boxes,100-Place, White

LC7006-100 100/CS
EUR 806

Microscope Slide Boxes,25-Place, Blue

LC7007-001 EA
EUR 66

Microscope Slide Boxes,25-Place, Blue

LC7007-010 10/PK
EUR 124

Microscope Slide Boxes,25-Place, Blue

LC7007-100 100/CS
EUR 570

Microscope Slide Boxes,25-Place, Green

LC7008-001 EA
EUR 66

Microscope Slide Boxes,25-Place, Green

LC7008-010 10/PK
EUR 124

Microscope Slide Boxes,25-Place, Green

LC7008-100 100/CS
EUR 570

Microscope Slide Boxes,25-Place, Red

LC7009-001 EA
EUR 66

Microscope Slide Boxes,25-Place, Red

LC7009-010 10/PK
EUR 124

Microscope Slide Boxes,25-Place, Red

LC7009-100 100/CS
EUR 570

Microscope Slide Boxes,25-Place, Yellow

LC7010-001 EA
EUR 66

Microscope Slide Boxes,25-Place, Yellow

LC7010-010 10/PK
EUR 124

Microscope Slide Boxes,25-Place, Yellow

LC7010-100 100/CS
EUR 570

Microscope Slide Boxes,25-Place, White

LC7011-001 EA
EUR 66

Microscope Slide Boxes,25-Place, White

LC7011-010 10/PK
EUR 124

Microscope Slide Boxes,25-Place, White

LC7011-100 100/CS
EUR 570

PIK-90

B1671-25
EUR 485

PIK-90

B1671-5
EUR 158

PIK-90

E1KS1187 5mg
EUR 325

LR-90

HY-76383 10mM/1mL
EUR 497

PIK-90

HY-12030 5mg
EUR 108

PIK-90

A2764-10 10 mg
EUR 145
Description: PIK-90 is a broad-spectrum PI3K inhibitors that inhibits PI3K?, PI3K? and PI3K? with IC50 values of 11, 18 and 58nM, respectively [1].Studies showed that PIK-90 induced increased levels of secreted IgE at 1 ?mol/L, while it inhibited IgE production at doses of greater than 2 ?mol/L [2].

PIK-90

A2764-5 5 mg
EUR 118
Description: PIK-90 is a broad-spectrum PI3K inhibitors that inhibits PI3K?, PI3K? and PI3K? with IC50 values of 11, 18 and 58nM, respectively [1].Studies showed that PIK-90 induced increased levels of secreted IgE at 1 ?mol/L, while it inhibited IgE production at doses of greater than 2 ?mol/L [2].

IMR-90

P0016019 One Frozen vial
EUR 455

CometAssay High Throughput Slides

4252-02K-01 100 tests
EUR 1619.4

CometAssay Alkaline Control Slides

4256-010-CC 10 Tests
EUR 324.4

CometAssay Neutral Control Slides

4257-010-NC 10 Tests
EUR 324.4

Alcian Blue (Control Slides)

TCS0002-100 100 Slides
EUR 706

Alcian Blue (Control Slides)

TCS0002-25 25 Slides
EUR 232

Alcian Blue (Control Slides)

TCS0002-5 5 Slides
EUR 98

Helicobacter Pylori (Control Slides)

TCS0011-100 100 Slides
EUR 706

Helicobacter Pylori (Control Slides)

TCS0011-25 25 Slides
EUR 232

Helicobacter Pylori (Control Slides)

TCS0011-5 5 Slides
EUR 98

Iron - Liver (Control Slides)

TCS0013-100 100 Slides
EUR 706

Iron - Liver (Control Slides)

TCS0013-25 25 Slides
EUR 232

Iron - Liver (Control Slides)

TCS0013-5 5 Slides
EUR 98

Mast Cell (Control Slides)

TCS0014-100 100 Slides
EUR 706

Mast Cell (Control Slides)

TCS0014-25 25 Slides
EUR 232

Mast Cell (Control Slides)

TCS0014-5 5 Slides
EUR 98

PAS/Glycogen (Control Slides)

TCS0017-100 100 Slides
EUR 706

PAS/Glycogen (Control Slides)

TCS0017-25 25 Slides
EUR 232

PAS/Glycogen (Control Slides)

TCS0017-5 5 Slides
EUR 98

Trichrome - Colon (Control Slides)

TCS0021-100 100 Slides
EUR 706

Trichrome - Colon (Control Slides)

TCS0021-25 25 Slides
EUR 232

Trichrome - Colon (Control Slides)

TCS0021-5 5 Slides
EUR 98

Trichrome - Liver (Control Slides)

TCS0022-100 100 Slides
EUR 706

Trichrome - Liver (Control Slides)

TCS0022-25 25 Slides
EUR 232

Trichrome - Liver (Control Slides)

TCS0022-5 5 Slides
EUR 98

TOMO Slides 1 Box

TOMO100 100 Slides
EUR 90

HSP-90/ Rat HSP- 90 ELISA Kit

ELA-E0863r 96 Tests
EUR 886

Chicken Heat Shock Protein 90 (HSP-90)

QY-E80144 96T
EUR 426

Plain Glass Plates 2/pk, RS

2392031 8unit
EUR 283
Description: Rect angul ar wi t hout not ch & si de-spacer s

Plain Glass Plates 2/pk, CP

2393637 15unit
EUR 276

EDTA plasma

90-1009 100 ml
EUR 2028
Description: BNP and NT-proBNP free plasma EDTA

Defibrinated Human Serum

90-1010 10 ml
EUR 127
Description: Defibrinated Human Serum for use in Controls

Human Serum (AB positive)

90-1011 100 ml
EUR 484
Description: Pooled Normal Human Serum (AB positive)

Normal Human Serum (filtered)

90-1013 100 ml
EUR 548
Description: Human Serum Off The Clot from Normal Donors

Human Plasma Pooled Unfiltered K2-EDTA

90-1015 100 ml
EUR 336
Description: Unfiltered, pooled human plasma K2-EDTA

Lithium Heparin Plasma

90-1016 100 ml
EUR 370
Description: Lithium Heparin Human Plasma Sterile filtered

Non Sterile LH Human Plasma

90-1017 250 ml
EUR 851
Description: Non Sterile Lithium Heparin Human Plasma

Human Serum Albumin (PSA Elevated)

90-CS1084 1 ml
EUR 133
Description: Human Serum Albumin (PSA Elevated)

Pipette Bulb Filler, 90ml

PF003-90 1 UNIT
EUR 51.74
  • Product category: Labware/Pipettes - Serological/Bulbs/Fillers

90X15mm Cell Culture Plastic Dishes 10/PK

DS001-90 1PK, 10UNIT
EUR 54.18
  • Product category: Labware/Culture Related/Culture Dishes

Ro 90-7501

B7043-10 10 mg
EUR 242

Ro 90-7501

B7043-25 25 mg
EUR 508

Ro 90-7501

B7043-5 5 mg
EUR 151

Ro 90-7501

B7043-50 50 mg
EUR 863

20-Hydroxyecdysone, 90%

GP0436-1G 1 g
EUR 102

20-Hydroxyecdysone, 90%

GP0436-250MG 250 mg
EUR 62

MBP (90-106)

5-01524 4 x 1mg Ask for price

Rnase (90–105)

5-01885 4 x 1mg Ask for price

130233-90-8

TBZ0281 unit Ask for price

Solid Block (for slides / machining)

BSW01 1 PC
EUR 182.23
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Solid Block (for slides / machining)

BCM1412-BSW01 1 pcs, 1 UNIT
EUR 194.34
  • Product category: Benchtop Equipment/Heat Baths/Heat Blocks

Human Heat Shock Protein 90(HSP-90)ELISA Kit

GA-E1821HM-48T 48T
EUR 289

Human Heat Shock Protein 90(HSP-90)ELISA Kit

GA-E1821HM-96T 96T
EUR 466

Mouse Heat Shock Protein 90(Hsp-90)ELISA Kit

GA-E0314MS-48T 48T
EUR 336

Mouse Heat Shock Protein 90(Hsp-90)ELISA Kit

GA-E0314MS-96T 96T
EUR 534

Rat heat shock protein 90(hSP-90)ELISA Kit

GA-E0531RT-48T 48T
EUR 317

Rat heat shock protein 90(hSP-90)ELISA Kit

GA-E0531RT-96T 96T
EUR 496

Rabbit Heat Shock Protein 90,HSP-90 ELISA Kit

GA-E0090RB-48T 48T
EUR 326

Rabbit Heat Shock Protein 90,HSP-90 ELISA Kit

GA-E0090RB-96T 96T
EUR 524

Rat HSP-90(Heat Shock Protein 90) ELISA Kit

ER1058 96T
EUR 476.25
  • Detection range: 125-8000 pg/ml
  • Alias: HSP-90(Heat Shock Protein 90)/HSP90AA1/Hsp90/HSP86/HSP89A/HSP90A/HSP90N/HSPC1/HSPCA/HSPCAL1/HSPCAL4/HSPN/LAP2/FLJ31884/Heat shock 86 kDa/heat shock 90kD protein 1, alpha/heat shock 90kD protein 1, alpha-like 4/h
  • Show more
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 75pg/ml

Mouse HSP-90(Heat Shock Protein 90) ELISA Kit

EM1138 96T
EUR 524.1
  • Detection range: 0.156-10 ng/ml
  • Alias: HSP-90(Heat Shock Protein 90)/HSP90AA1/Hsp90/HSP86/HSP89A/HSP90A/HSP90N/HSPC1/HSPCA/HSPCAL1/HSPCAL4/HSPN/LAP2/FLJ31884/Heat shock 86 kDa/heat shock 90kD protein 1, alpha/heat shock 90kD protein 1, alpha-like 4/h
  • Show more
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml

Human Heat Shock Protein 90 (HSP-90) CLIA Kit

abx195773-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Mouse Heat Shock Protein 90 (HSP-90) CLIA Kit

abx195774-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Rat Heat Shock Protein 90 (HSP-90) CLIA Kit

abx195775-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Human Heat Shock Protein 90,HSP-90 ELISA Kit

201-12-1805 96 tests
EUR 440
  • This Heat Shock Protein 90 ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Mouse Heat Shock Protein 90, Hsp-90 ELISA Kit

CSB-E08312m-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Mouse Heat Shock Protein 90, Hsp-90 in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Heat Shock Protein 90, Hsp-90 ELISA Kit

1-CSB-E08312m
  • EUR 946.00
  • EUR 5782.00
  • EUR 3060.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Mouse Heat Shock Protein 90, Hsp-90 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Chicken Heat Shock Protein 90, HSP-90 ELISA Kit

CSB-E12873C-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Chicken Heat Shock Protein 90, HSP-90 in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Chicken Heat Shock Protein 90, HSP-90 ELISA Kit

1-CSB-E12873C
  • EUR 900.00
  • EUR 5476.00
  • EUR 2900.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Chicken Heat Shock Protein 90, HSP-90 in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

PoFAine Heat Shock Protein 90,HSP-90 ELISA Kit

CN-01297P1 96T
EUR 457

PoFAine Heat Shock Protein 90,HSP-90 ELISA Kit

CN-01297P2 48T
EUR 306

Rat heat shock protein 90,hSP-90 ELISA Kit

CN-01862R1 96T
EUR 449

Mouse Heat Shock Protein 90,Hsp-90 ELISA Kit

CN-02740M1 96T
EUR 457

Mouse Heat Shock Protein 90,Hsp-90 ELISA Kit

CN-02740M2 48T
EUR 306

Human Heat Shock Protein 90,HSP-90 ELISA Kit

CN-03553H1 96T
EUR 473

Human Heat Shock Protein 90,HSP-90 ELISA Kit

CN-03553H2 48T
EUR 323

Rabbit Heat Shock Protein 90,HSP-90 ELISA Kit

CN-00634R1 96T
EUR 461

Rabbit Heat Shock Protein 90,HSP-90 ELISA Kit

CN-00634R2 48T
EUR 310

Human Heat Shock Protein 90(HSP-90)ELISA Kit

QY-E01784 96T
EUR 361

Rat heat shock protein 90(hSP-90)ELISA Kit

QY-E11121 96T
EUR 400

Goat Heat Shock Protein 90 (HSP-90) ELISA Kit

QY-E140075 96T
EUR 413

Rabbit Heat Shock Protein 90,HSP-90 ELISA Kit

QY-E30209 96T
EUR 374

Porcine heat shock protein 90 (HSP-90) ELISA Kit

QY-E40083 96T
EUR 400

Mouse heat shock protein 90(hSP-90)ELISA Kit

QY-E20717 96T
EUR 361

plain glass plates 33x45 cm pk/2

ESEQ1100-GLASS ea
EUR 116

plain glass plates 20x50 cm pk/2

ESEQ1200-GLASS ea
EUR 108

DiagPoly Plain Polystyrene Particles, 0.05-0.1 µm

DNM-P001 10 mL
EUR 554

DiagPoly Plain Polystyrene Particles, 0.1-0.2 µm

DNM-P002 10 mL
EUR 554

DiagPoly Plain Polystyrene Particles, 0.2-0.3 µm

DNM-P003 10 mL
EUR 554

Utilities spray droplets in situ analysis and real-time monitoring of chemical reactions indicated by bis (cyclopentadienyl) zirconium dichloride (zirconocene dichloride) / methylaluminoxane, Cp2ZrCl2 / MAO, homogeneous ethylene polymerization catalyst in a variety of solutions. reaction time ranging from seconds to minutes, and the catalyst active species and products of ethylene polymers obtained and identified by tandem mass spectrometry.

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