A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.

A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.

In this paper we report the development of a modular, cost-effective open source, and fully automated slide-scanning microscope, composed entirely of readily available parts of the off-the-shelf, and capable of bright field and fluorescence mode.

Automatic X-Y stage consists of two steps Cheap micrometer coupled with the stepper motor is operated in open loop mode. The microscope consists of low cost CMOS sensor and lens’s board placed in the 4f configuration. This system has a resolution of about 1 micron, is limited by the f / # lens boards are available. The microscope is compact, measuring just 25 × 25 × 30 cm, and have the absolute positioning accuracy of ± 1 m in the X and the direction Y. A Z-phase imaging allows autofocus and a wider field of view even on non-planar samples, and custom software enables automatic determination of the boundaries of the sample and image mosaicking.

We demonstrate the utility of our devices through the blood imaging fluorescent- and transmission-dye stained and smears feces containing parasite of humans and animals, as well as some tissue samples ready. These results indicate that the image quality comparable to high-end commercial microscopes with a cost of less than US $ 400 for the bright-field system, with an additional US $ 100 is required for the fluorescence module.


In the current trend of miniaturization and simplification of imaging flow cytometry, Lab-on-a-Chip (LOC) of the microfluidic device represents an innovative and cost-effective solutions. Within this framework, we propose for the first time the platform novel based on the compactness of the microscope slide holographic (HMS) in combination with computing features the new space-time holography digital (STDH) using a 1D linear sensor array (LSA) is not a 2D CCD or CMOS camera to respond real diagnostic needs. In this LoC platform, computational methods, holography, and microfluidics interlinked to provide an imaging system by reducing the number of optical components and the ability to achieve a reliable cell counting even in the absence of highly accurate flow control. STDH utilize motion sample into a microfluidic channel to get the field-of-view is limited along the direction of flow, independent of magnification factor.

Furthermore, the typical numerical refocusing from modality hologram allows imaging and visualization of the entire volume of the channel, thus avoiding the loss of information due to the limited depth of focus of a standard microscope. As a result, we believe that this platform could open new perspectives to improve throughput by 3D volumetric imaging.

 A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.
A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.

Droplet spray ionization of a glass microscope slide: real-time monitoring of polymerization of ethylene.

Ambient ionization mass spectrometry is achieved in a simple manner by loading a sample solution onto a glass microscope cover corner is positioned in front of the inlet to the mass spectrometer and applying a high voltage to the sample. The resulting flow of charged droplets is stable, has no contamination from the substrate platform, and can be used repeatedly.

Premium Plain Glass Microscope Slides, 144 /pk, 1 mm thickness, 25 mm wide by 73 mm long

M-ACC-GSL9 200 TIPS
EUR 65
Description: Premium Plain Glass Microscope Slides, 144 /pk, 1 mm thickness, 25 mm wide by 73 mm long

Microscope Slides, Micropure

22245-20 1box
EUR 380

Microscope Slides, Micropure

22245-1 1box
EUR 32

Slides 90o Ground Plain 1mm Thick

MIC3804 PK50
EUR 8.75

Vinyl microscope slides RINZL

DD52951 PK144
EUR 24.62

Super Frosted Microscope Slides

21912-1 1box
EUR 60

Slides 90o Ground Plain 1-1.2mm Thick

MIC3812 PK50
EUR 7.68

Microscope Slides, Immunofluorescence

18357-1 1box
EUR 168

Slides 90o Ground Plain 0.8-1mm Thick

MIC3810 PK50
EUR 7.68

Polysine Coated Microscope Slides

26414-1 1box
EUR 82

Polysine Coated Microscope Slides

26414-20 20box
EUR 585

Superfrost® Plus microscope slides

PO103 box (72 slides)
EUR 123.6

Proteinase-resistant microscope slides

PO102 100 slides
EUR 236.4

Tissue Tack (+ Charged) Microscope Slides

24216-1 1box
EUR 55

Tissue Tack (+ Charged) Microscope Slides

24216-20 20box
EUR 720

Cardboard tray for 20 microscope slides

DD65205 EACH
EUR 19.2

Plastic box for 100 microscope slides (red colour)

DD37503 EACH
EUR 38.4

Plastic box for 100 microscope slides (blue colour)

DD37501 EACH
EUR 38.4

Box for 25 microscope slides purple colour

DD49719 EACH
EUR 19.2

Box for 25 microscope slides orange colour

DD49720 EACH
EUR 19.2

Plastic box for 50 microscope slides (blue colour)

DD37507 EACH
EUR 32.4

TruBond 380 Adhesive Microscope Slides, 1000 pcs/case

IW-T380C -
EUR 385

TruBond 380 Adhesive Microscope Slides, 100 pcs/box

IW-T380 -
EUR 45

25-place microscope slide box (for 75 x 25 mm slides)

PO202 each
EUR 73.2

Box for 25 microscope slides turquoise colour

DD49721 EACH
EUR 19.2

100-place microscope slide box (for 75 x 25 mm slides)

PO201 each
EUR 84

100-place microscope slide box (for 75 x 50 mm slides)

PO203 each
EUR 152.4

Gelatin-coated microscope slides (75 x 25 mm)

PO101 100 slides
EUR 236.4

Gelatin-coated microscope slides (75 x 50 mm)

PO105 100 slides
EUR 420

Polystyrene box for 100 microscope slides blue colour

DD139537 EACH
EUR 22.8

Microscope slides, positive charged, 90° corners, frosted on one end, 25 x 75mm

M7100-90 72/pack
EUR 75.4
Description: Microscope slides positive charged

Polystyrene box for 100 microscope slides green colour

DD139538 EACH
EUR 22.8

Plastic box for 100 microscope slides (assorted colours) cork bottom

DD10197 PK5
EUR 87.6

Microscope slide dispenser for box of 72 standard 25 x 75mm slides

M7100-SD 1/pack
EUR 83.89
Description: Microscope slide dispenser

AutoFrost Enhanced IHC Microscope Slides, Clipped corner, White, 3000pcs/case

EMP00W3 -
EUR 1250

Microscope slides, positive charged, 45° corners, frosted on one end, 25 x 75mm

M7100-45 72/pack
EUR 75.4
Description: Microscope slides positive charged

Microscope Slide Box 100 Place Tall

MIC4080 EACH
EUR 42

Microscope Slide Coverslips, Plastic

16601-1 1box
EUR 148

Microscope Slide Holder Block

24764-1 1each
EUR 148

Microscope Slide Mailing Tubes

23998-10 10pkg
EUR 40

Microscope Slide Boxes,100-Place, Red

LC7004-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, Red

LC7004-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, Red

LC7004-100 100/CS
EUR 967.2

Microscope Slide Boxes,25-Place, Red

LC7009-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Red

LC7009-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Red

LC7009-100 100/CS
EUR 684

Microscope Slide Boxes,100-Place, Blue

LC7002-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, Blue

LC7002-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, Blue

LC7002-100 100/CS
EUR 967.2

Microscope Slide Boxes,25-Place, Blue

LC7007-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Blue

LC7007-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Blue

LC7007-100 100/CS
EUR 684

Microscope Slide Box True North

MIC4090 EACH
EUR 47.52

Microscope Slide Storage Cabinet

22352-1 1each
EUR 278

Microscope Slide Boxes,100-Place, Green

LC7003-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, Green

LC7003-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, Green

LC7003-100 100/CS
EUR 967.2

Microscope Slide Boxes,100-Place, White

LC7006-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, White

LC7006-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, White

LC7006-100 100/CS
EUR 967.2

Microscope Slide Boxes,25-Place, Green

LC7008-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Green

LC7008-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Green

LC7008-100 100/CS
EUR 684

Microscope Slide Boxes,25-Place, White

LC7011-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, White

LC7011-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, White

LC7011-100 100/CS
EUR 684

Microscope Slide Box 25 ULT safe

MIC4092 EACH
EUR 19.37

Microscope Slide Boxes,100-Place, Yellow

LC7005-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, Yellow

LC7005-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, Yellow

LC7005-100 100/CS
EUR 967.2

Microscope Slide Boxes,25-Place, Yellow

LC7010-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Yellow

LC7010-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Yellow

LC7010-100 100/CS
EUR 684

Microscope Slide Polysine 75x25x1mm

MIC3050 PK72
EUR 43.22

2 Place Microscope Slide Mailer PP Natural

MIC3172 PK25
EUR 46.92

Microscope Slide Holder, Peel-A-Way

19801A-1 1Box
EUR 56

Microscope Slide Storage Box, Plastic small box

16603-1 1bag
EUR 28

Microscope Slide Storage Box, Plastic large box

16604-1 1each
EUR 54

Premium Microscope Slide Box 50-Place Cork Red

MIC4216 EACH
EUR 21.46

Premium Microscope Slide Box 50-Place Cork Blue

MIC4214 EACH
EUR 26.52

Microscope Slide Storage Cabinet Base Unit

22353-1 1each
EUR 397

2 Place Microscope Slide Mailer PP Assorted Colours

MIC3170 PK25
EUR 48

Microscope Slide Coverslips, Glass, 22mm x 30mm

23999-1 1oz
EUR 40

Microscope Slide Coverslips, Glass, 24mm x 40mm

24070-1 1oz
EUR 40

Microscope Slide Coverslips, Glass, 24mm x 50mm

24071-1 1oz
EUR 41

Microscope Slide Coverslips, Glass, 24mm x 60mm

24072-1 1oz
EUR 53

Microscope Slide Coverslips, Glass, 22mm x 22mm

21913-1 1oz
EUR 40

Quartz Microscope Slide, 1"x2" (25.4 x 50.8 mm) x 0.5 mm Thin

24962-1 1EA
EUR 217

Quartz Microscope Slide, 1"x3" (25.4 x 76.2 mm) x 0.5 mm Thin

24963-1 1EA
EUR 192

Quartz Microscope Slide, 1"x1" (25.4 x 25.4 mm) x 1 mm Thick

24957-1 1EA
EUR 58

Slides Cut Edge Plain 1mm Thick

MIC3800 PK50
EUR 4.8

Slides 90o Ground Twin Frost 1mm Thick

MIC3806 PK50
EUR 9.92

Microscope Cover Slips No. 1.5 16mm

MIC2182 PK100
EUR 10.8

Microscope Cover Slips No. 0 18x18mm

MIC2158 PK100
EUR 3.6

Microscope Cover Slips No. 0 22x22mm

MIC2160 PK100
EUR 3.6

Microscope Cover Slips No. 1 18x18mm

MIC2162 PK100
EUR 2.4

Microscope Cover Slips No. 1 22x22mm

MIC2164 PK100
EUR 3.6

Microscope Cover Slips No. 1.5 18x18mm

MIC2166 PK100
EUR 2.4

Microscope Cover Slips No. 1.5 22x22mm

MIC2168 PK100
EUR 3.6

Microscope Cover Slips No.1.5 22x26mm

MIC2170 PK100
EUR 4.8

Microscope Cover Slips No. 1.5 22x32mm

MIC2172 PK100
EUR 4.8

Microscope Cover Slips No. 1.5 22x40mm

MIC2174 PK100
EUR 6

Microscope Cover Slips No. 2 18x18mm

MIC2176 PK100
EUR 3.6

Microscope Cover Slips No. 2 22x22mm

MIC2178 PK100
EUR 4.8

Microscope Cover Slips No. 0 19mm dia

MIC2180 PK100
EUR 12

Stereo Microscope Trinocular

MIC1590 EACH
EUR 1274.27

Upright Fluorescence Microscope

25039-1 1EA
EUR 19098

Inverted Fluorescence Microscope

25040-1 1EA
EUR 15134

Motic SMZ-168-BL Microscope

MIC2800 EACH
EUR 1357.74

BA310 Elite Trinocular microscope

MIC2937 EACH
EUR 2667.34

BA210E Elite Binocular Microscope

MIC5633 EACH
EUR 1809.56

Microscope Pack 1 CX23 100x Oil

MIC1968 EACH
EUR 1827.42

Microscope Pack 2 CX23 BIN LED

MIC1970 EACH
EUR 1658.11

Microscope Binoc MAGNUM-B planachrom obj

MIC0105 EACH
EUR 2008.8

Microscope Trinoc MAGNUM-T planachrom obj

MIC0107 EACH
EUR 2085.6

Digi-Pad Microscope Tablet Camera

MIC0115 EACH
EUR 1807.2

Motic BA210 LED Microscope Binocular

MIC2004 EACH
EUR 1667.25

Motic BA310 LED Microscope Binocular

MIC2016 EACH
EUR 2250.85

ReadiUseâ„¢ microscope mounting solution

20009-50mL 50 mL
EUR 86
Description: The mounting medium is the solution in which the specimen is embedded, generally under a cover glass.

Motic BA310 LED Microscope Trinocular

MIC2018 EACH
EUR 2545.92

VIKEM(R) VINYL MICROSCOPE COVER EXTRA

BAF243040000-1EA EACH
EUR 32.88

MIGHTY SCOPE(TM) DIGITAL MICROSCOPE 5M

Z742668-1EA EACH
EUR 451.74

ReadiUse™ microscope mounting solution

20009 50 mL
EUR 86

Motic SMZ-140-N2GG Stereo Microscope

MIC5900 EACH
EUR 774.22

Motic SMZ-143-N2GG Stereo Microscope

MIC5902 EACH
EUR 900.86

SMZ-171-Binocular P Stero Microscope

MIC5446 EACH
EUR 1552.36

SMZ-171-Binocular LED Stero Microscope

MIC5444 EACH
EUR 1989.73

SMZ-171-Trinocular P Stero Microscope

MIC5447 EACH
EUR 1668.56

SMZ-171-Trinocular LED Stero Microscope

MIC5445 EACH
EUR 2108.54

Microscope Binoc MAGNUM-B pos phase contrast

MIC0106 EACH
EUR 2624.4

SLS Lab Basics Star-24 LED Stereo Microscope

MIC2026 EACH
EUR 1422

Microscope Trinoc MAGNUM-T pos phase contrast

MIC0108 EACH
EUR 2865.6

18 mm Circular Glass Cover Slides - Plain, 1000/CS

M-CSL18-103 1000 pcs.
EUR 228
Description: 18 mm Circular Glass Cover Slides - Plain, 1000/CS

22 mm Circular Glass Cover Slides - Plain, 1000/CS

M-CSL22-104 1000 pcs.
EUR 282
Description: 22 mm Circular Glass Cover Slides - Plain, 1000/CS

Economical slide with cut edges

DD68763 PK50
EUR 7.2

MiTeGen Kit V201 Stereo Microscope kit for MicroCrystallography:

ZV20-MTGKV201 1 KIT
EUR 16532
Description: MiTeGen Kit V201 Stereo Microscope kit for MicroCrystallography:- Ergonomic adjustable eyepieces- A wide array of accessories available- 7.5 to 150 x magnification range- ZEISS SteREO Discovery.V20- ZEISS SteREO Discovery.V20 body,- Human Interface Panel HIP,- Dust Protection Set M ,- Binoc Phototube Ergo Stereo 5-45 ,- Eyepiece PL 10x/23 Br foc ,- Folding Eyecup ,- Stand base Profile S,- Transmitted Light Equipment S- Cold-light source Zeiss CL6000 LED- 6200K color temperature,- control of intensity & 6 memory positions,- filter slider for 2 filters 35x26x4mm (sold separately)- Analyzer S Rotatable,- Polarizer D =84mm,- Coarse/fine drive w/column S 490mm- Mount S with 76 mm Diameter Support- Achromat S 1.0x Reo WD=63 lens

MiTeGen Kit V81 Stereo Microscope kit for Crystallography:

ZV20-MTGKV81 1 KIT
EUR 13998
Description: MiTeGen Kit V81 Stereo Microscope kit for Crystallography:- ZEISS Stereo Discovery V8 Microscope:- ZEISS Stereo Discovery.V8 body,- Dust Protection Set M ,- Binocular Phototube Ergo Stereo 5-45 ,- Eyepiece PL 10x/23 Br foc ,- Folding Eyecup ,- Stand base Profile S,- Transmitted Light Equipment S:- Cold-light source ZEISS CL6000 LED- 6200K color temperature, control of intensity & 6 memory positions,- filter slider for 2 filters (35 x 26 x 4mm, (filters sold separately)- Analyzer S Rotatable,- Polarizer D =84mm,- Manual focus drive fa/ Discovery,- Mount S with 76 mm Diameter Support- Achromatic S 1.0x Reo WD=63 lens

MiTeGen Kit V121 Stereo Microscope kit for Crystallography:

ZV20-MTGKV121 1 KIT
EUR 15282
Description: MiTeGen Kit V121 Stereo Microscope kit for Crystallography:- ZEISS Stereo Discovery V12 Microscope:- ZEISS Stereo Discovery.V8 body,- Dust Protection Set M ,- Binocular Phototube Ergo Stereo 5-45 ,- Eyepiece PL 10x/23 Br foc ,- Folding Eyecup ,- Stand base Profile S,- Transmitted Light Equipment S:- Cold-light source ZEISS CL6000 LED- 6200K color temperature,- control of intensity & 6 memory positions,- filter slider for 2 filters (35 x 26 x 4mm, (filters sold separately)- Analyzer S Rotatable,- Polarizer D =84mm,- Manual focus drive fa/ Discovery,- Mount S with 76 mm Diameter Support- Achromatic S 1.0x Reo WD=63 lens

Max III Trinocular Compound Microscope with Plan Achromatic Objectives

SLS2272 EACH
EUR 1525.2

MiTeGen Kit Stemi 305 Stereo Microscope kit for Crystallography:

ZV20-MTGK305 1 KIT
EUR 2589
Description: MiTeGen Kit Stemi 305 Stereo Microscope kit for Crystallography:Stemi 305 trinocular body w/ integrated camera portIncludes adapter for C-mount lensesStand K Edu w/ integrated transmissive LED cold light and polarizerSpot illuminator w/ polarizerAnalyzer (rotatable)

MiTeGen Crystallography Microscope Kit for Protein Crystallography:

ZV20-MTGK508-PC1 1 KIT
EUR 6492
Description: MiTeGen Crystallography Microscope Kit for Protein Crystallography:- Stemi 508 Stand M- Magnification: 10-80x (with 16x eyepieces)- Free Working Distance: 92 mm- Eyepieces (2 x 10x & 2 x 16x)- Cold light (LED) transillumination source with large working area stand- Rotatable and slidable mirror for brightfield, darkfield and oblique transillumination- Plain mirror side for crisp, diffuse mirror side for homogeneous illumination- Trinocular Camera Port Configuration- Camera Adapter 60N- Viewing angle 35 degrees with adjustable interocular distance- Camera Port- Analyzer (rotatable)- Transillumination Polarizer- Dual flex-neck top-side LED illumination

Max III Trinocular Compound Microscope with Achromatic Objectives

SLS2270 EACH
EUR 1194

Magtex Trinocular Materials and Biological Microscope with Plan Achromatic Objectives

SLS2280 EACH
EUR 4160.4

Steddy-T Trinocular Stereo Microscope with Diamond Base with Zoom Ratio 0.67x - 4.5x

SLS2106 EACH
EUR 1638

Magnum Binocular Compound Microscope with Infinity Corrected Plan Achromatic Objectives with LED Illumination

MIC0122 EACH
EUR 2385.6

Magtex Trinocular Reflected and Transmitted Light Microscope with Polarisation and Plan Achromatic Objectives

SLS2282 EACH
EUR 6100.8

Magnum Trinocular Compound Microscope with Infinity Corrected Plan Achromatic Objectives with LED Illumination

MIC0124 EACH
EUR 2496

C-Mount for All SLS Lab Basics Max III Microscopes

SLS2300 EACH
EUR 127.2

C-Mount for All SLS Lab Basics Magnum and Magtex Microscopes

SLS2302 EACH
EUR 145.2

Ground Joint Socket

8124/24/29 EACH
EUR 4.92

Slides Cut Edge Twin Frost 1mm Thick

MIC3802 PK50
EUR 5.88

DUAL BLOCK, 96 WELL MICROTITER PLATE OR 4 SLIDES

480131 1/pk
EUR 261.6
Description: Lab Equipment; Constant Temperature Equipment

Ground Sleeve Gland St20/2

QST20/2 EACH
EUR 136.8

Electrode ground joint-junction

TIT3120 EACH
EUR 394.29

Micro Slides Tropical Frosted One End One Side

MIC1008 PK50
EUR 3.6

Watch Glass 50mm Fused Edges

213213207 PK10
EUR 118.8

Watch Glass 60ml Fused Edges

2132134 PK10
EUR 141.6

Aspirator Pyrex Ground Side 5Ltr

ASP1060 EACH
EUR 199.2

Aspirator Pyrex Ground Side 10Lt

ASP1065 EACH
EUR 362.4

Aspirator Pyrex Ground Side 20Lt

ASP1070 EACH
EUR 583.2

Nanosoft Clipping Station for the clipping of cryoEM grids for Thermo Fisher autoloader-based microscopes.

M-CEM-NS-CLSTN 1 UNIT
EUR 2400
Description: Nanosoft Clipping Station for the clipping of cryoEM grids for Thermo Fisher autoloader-based microscopes.

Nanosoft Clipping Station for the clipping of cryoEM grids for Thermo Fisher autoloader-based microscopes.

M-CEM-NS-CLSTN01 each
EUR 2400
Description: Nanosoft Clipping Station for the clipping of cryoEM grids for Thermo Fisher autoloader-based microscopes.

Fluorescence Microscopy Aid

4400 3 ml
EUR 216.6
Description: This is Counter - Stain/Blocking Diluent with optimal formula for preparaiton of final dilutions of FITC conjugate prior to use in FA procedures.

Quickfit Ground Socket Conical Tube 10ml

QBC24C14T0 PK10
EUR 244.8

Lugols Iodine Solution (for Microscopy)

RRSP84-D 500ml
EUR 7.33

Utilities spray droplets in situ analysis and real-time monitoring of chemical reactions indicated by bis (cyclopentadienyl) zirconium dichloride (zirconocene dichloride) / methylaluminoxane, Cp2ZrCl2 / MAO, homogeneous ethylene polymerization catalyst in a variety of solutions. reaction time ranging from seconds to minutes, and the catalyst active species and products of ethylene polymers obtained and identified by tandem mass spectrometry.

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