A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.

A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.

In this paper we report the development of a modular, cost-effective open source, and fully automated slide-scanning microscope, composed entirely of readily available parts of the off-the-shelf, and capable of bright field and fluorescence mode.

Automatic X-Y stage consists of two steps Cheap micrometer coupled with the stepper motor is operated in open loop mode. The microscope consists of low cost CMOS sensor and lens’s board placed in the 4f configuration. This system has a resolution of about 1 micron, is limited by the f / # lens boards are available. The microscope is compact, measuring just 25 × 25 × 30 cm, and have the absolute positioning accuracy of ± 1 m in the X and the direction Y. A Z-phase imaging allows autofocus and a wider field of view even on non-planar samples, and custom software enables automatic determination of the boundaries of the sample and image mosaicking.

We demonstrate the utility of our devices through the blood imaging fluorescent- and transmission-dye stained and smears feces containing parasite of humans and animals, as well as some tissue samples ready. These results indicate that the image quality comparable to high-end commercial microscopes with a cost of less than US $ 400 for the bright-field system, with an additional US $ 100 is required for the fluorescence module.


In the current trend of miniaturization and simplification of imaging flow cytometry, Lab-on-a-Chip (LOC) of the microfluidic device represents an innovative and cost-effective solutions. Within this framework, we propose for the first time the platform novel based on the compactness of the microscope slide holographic (HMS) in combination with computing features the new space-time holography digital (STDH) using a 1D linear sensor array (LSA) is not a 2D CCD or CMOS camera to respond real diagnostic needs. In this LoC platform, computational methods, holography, and microfluidics interlinked to provide an imaging system by reducing the number of optical components and the ability to achieve a reliable cell counting even in the absence of highly accurate flow control. STDH utilize motion sample into a microfluidic channel to get the field-of-view is limited along the direction of flow, independent of magnification factor.

Furthermore, the typical numerical refocusing from modality hologram allows imaging and visualization of the entire volume of the channel, thus avoiding the loss of information due to the limited depth of focus of a standard microscope. As a result, we believe that this platform could open new perspectives to improve throughput by 3D volumetric imaging.

 A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.
A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.

Droplet spray ionization of a glass microscope slide: real-time monitoring of polymerization of ethylene.

Ambient ionization mass spectrometry is achieved in a simple manner by loading a sample solution onto a glass microscope cover corner is positioned in front of the inlet to the mass spectrometer and applying a high voltage to the sample. The resulting flow of charged droplets is stable, has no contamination from the substrate platform, and can be used repeatedly.

Gelatin-coated microscope slides (75 x 50 mm)

PO105 100 slides
EUR 334.25
Description: Best quality products

DiagNano Fluorophore Labeled Gold Nanoparticles, 90 nm

GFL-90 1 mL
EUR 1053

100-place microscope slide box (for 75 x 25 mm slides)

PO201 each
EUR 67.03
Description: Best quality products

25-place microscope slide box (for 75 x 25 mm slides)

PO202 each
EUR 59.1
Description: Best quality products

100-place microscope slide box (for 75 x 50 mm slides)

PO203 each
EUR 114.36
Description: Best quality products

Microscope slide dispenser for box of 72 standard 25 x 75mm slides

M7100-SD 1/pack
EUR 69.91
Description: Microscope slide dispenser

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 90 nm

GCK-M-90 1 kit
EUR 757

Custom Peptide Synthesis (>90%; mass spec, hplc (mg-kg size, price based upon peptide size: 2-100 aa)

PEP-90 1 Ask for price

Microscope slides, positive charged, 45° corners, frosted on one end, 25 x 75mm

M7100-45 72/pack
EUR 62.83
Description: Microscope slides positive charged

HEV-IgM Hepatitis E IgM ELISA test

90 96T/Box Ask for price
  • Area of application: Hepatitis testing
Description: ELISA based test for quantitative detection of HEV-IgM Hepatitis E IgM

Positive control tissue section for each antibody; Based on availability INQUIRE

Control-Slides Set of 5
EUR 176

ReadiUse™ microscope mounting solution

20009 50 mL
EUR 115
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Amyloid (Control Slides)

TCS0003-100 100 Slides
EUR 706

Amyloid (Control Slides)

TCS0003-25 25 Slides
EUR 232

Amyloid (Control Slides)

TCS0003-5 5 Slides
EUR 98

Argentaffin (Control Slides)

TCS0004-100 100 Slides
EUR 706

Argentaffin (Control Slides)

TCS0004-25 25 Slides
EUR 232

Argentaffin (Control Slides)

TCS0004-5 5 Slides
EUR 98

Fungus (Control Slides)

TCS0005-100 100 Slides
EUR 706

Fungus (Control Slides)

TCS0005-25 25 Slides
EUR 232

Fungus (Control Slides)

TCS0005-5 5 Slides
EUR 98

Bielschowsky's (Control Slides)

TCS0006-100 100 Slides
EUR 706

Bielschowsky's (Control Slides)

TCS0006-25 25 Slides
EUR 232

Bielschowsky's (Control Slides)

TCS0006-5 5 Slides
EUR 98

Calcium (Control Slides)

TCS0007-100 100 Slides
EUR 706

Calcium (Control Slides)

TCS0007-25 25 Slides
EUR 232

Calcium (Control Slides)

TCS0007-5 5 Slides
EUR 98

Elastic (Control Slides)

TCS0008-100 100 Slides
EUR 706

Elastic (Control Slides)

TCS0008-25 25 Slides
EUR 232

Elastic (Control Slides)

TCS0008-5 5 Slides
EUR 98

Melanin (Control Slides)

TCS0015-100 100 Slides
EUR 706

Melanin (Control Slides)

TCS0015-25 25 Slides
EUR 232

Melanin (Control Slides)

TCS0015-5 5 Slides
EUR 98

Mucin (Control Slides)

TCS0016-100 100 Slides
EUR 706

Mucin (Control Slides)

TCS0016-25 25 Slides
EUR 232

Mucin (Control Slides)

TCS0016-5 5 Slides
EUR 98

Reticulum (Control Slides)

TCS0019-100 100 Slides
EUR 706

Reticulum (Control Slides)

TCS0019-25 25 Slides
EUR 232

Reticulum (Control Slides)

TCS0019-5 5 Slides
EUR 98

Microscope Slide Boxes,100-Place, Blue

LC7002-001 Ea
EUR 70

Microscope Slide Boxes,100-Place, Blue

LC7002-010 10/PK
EUR 151

Microscope Slide Boxes,100-Place, Blue

LC7002-100 100/CS
EUR 806

Microscope Slide Boxes,100-Place, Green

LC7003-001 Ea
EUR 70

Microscope Slide Boxes,100-Place, Green

LC7003-010 10/PK
EUR 151

Microscope Slide Boxes,100-Place, Green

LC7003-100 100/CS
EUR 806

Microscope Slide Boxes,100-Place, Red

LC7004-001 Ea
EUR 70

Microscope Slide Boxes,100-Place, Red

LC7004-010 10/PK
EUR 151

Microscope Slide Boxes,100-Place, Red

LC7004-100 100/CS
EUR 806

Microscope Slide Boxes,100-Place, Yellow

LC7005-001 Ea
EUR 70

Microscope Slide Boxes,100-Place, Yellow

LC7005-010 10/PK
EUR 151

Microscope Slide Boxes,100-Place, Yellow

LC7005-100 100/CS
EUR 806

Microscope Slide Boxes,100-Place, White

LC7006-001 Ea
EUR 70

Microscope Slide Boxes,100-Place, White

LC7006-010 10/PK
EUR 151

Microscope Slide Boxes,100-Place, White

LC7006-100 100/CS
EUR 806

Microscope Slide Boxes,25-Place, Blue

LC7007-001 EA
EUR 66

Microscope Slide Boxes,25-Place, Blue

LC7007-010 10/PK
EUR 124

Microscope Slide Boxes,25-Place, Blue

LC7007-100 100/CS
EUR 570

Microscope Slide Boxes,25-Place, Green

LC7008-001 EA
EUR 66

Microscope Slide Boxes,25-Place, Green

LC7008-010 10/PK
EUR 124

Microscope Slide Boxes,25-Place, Green

LC7008-100 100/CS
EUR 570

Microscope Slide Boxes,25-Place, Red

LC7009-001 EA
EUR 66

Microscope Slide Boxes,25-Place, Red

LC7009-010 10/PK
EUR 124

Microscope Slide Boxes,25-Place, Red

LC7009-100 100/CS
EUR 570

Microscope Slide Boxes,25-Place, Yellow

LC7010-001 EA
EUR 66

Microscope Slide Boxes,25-Place, Yellow

LC7010-010 10/PK
EUR 124

Microscope Slide Boxes,25-Place, Yellow

LC7010-100 100/CS
EUR 570

Microscope Slide Boxes,25-Place, White

LC7011-001 EA
EUR 66

Microscope Slide Boxes,25-Place, White

LC7011-010 10/PK
EUR 124

Microscope Slide Boxes,25-Place, White

LC7011-100 100/CS
EUR 570

PIK-90

B1671-25
EUR 485

PIK-90

B1671-5
EUR 158

PIK-90

E1KS1187 5mg
EUR 325

PIK-90

A2764-10 10 mg
EUR 145
Description: PIK-90 is a broad-spectrum PI3K inhibitors that inhibits PI3K?, PI3K? and PI3K? with IC50 values of 11, 18 and 58nM, respectively [1].Studies showed that PIK-90 induced increased levels of secreted IgE at 1 ?mol/L, while it inhibited IgE production at doses of greater than 2 ?mol/L [2].

PIK-90

A2764-5 5 mg
EUR 118
Description: PIK-90 is a broad-spectrum PI3K inhibitors that inhibits PI3K?, PI3K? and PI3K? with IC50 values of 11, 18 and 58nM, respectively [1].Studies showed that PIK-90 induced increased levels of secreted IgE at 1 ?mol/L, while it inhibited IgE production at doses of greater than 2 ?mol/L [2].

PIK-90

HY-12030 5mg
EUR 108

LR-90

HY-76383 10mM/1mL
EUR 497

IMR-90

P0016019 One Frozen vial
EUR 455

CometAssay High Throughput Slides

4252-02K-01 100 tests
EUR 1619.4

CometAssay Alkaline Control Slides

4256-010-CC 10 Tests
EUR 324.4

CometAssay Neutral Control Slides

4257-010-NC 10 Tests
EUR 324.4

Alcian Blue (Control Slides)

TCS0002-100 100 Slides
EUR 706

Alcian Blue (Control Slides)

TCS0002-25 25 Slides
EUR 232

Alcian Blue (Control Slides)

TCS0002-5 5 Slides
EUR 98

Helicobacter Pylori (Control Slides)

TCS0011-100 100 Slides
EUR 706

Helicobacter Pylori (Control Slides)

TCS0011-25 25 Slides
EUR 232

Helicobacter Pylori (Control Slides)

TCS0011-5 5 Slides
EUR 98

Iron - Liver (Control Slides)

TCS0013-100 100 Slides
EUR 706

Iron - Liver (Control Slides)

TCS0013-25 25 Slides
EUR 232

Iron - Liver (Control Slides)

TCS0013-5 5 Slides
EUR 98

Mast Cell (Control Slides)

TCS0014-100 100 Slides
EUR 706

Mast Cell (Control Slides)

TCS0014-25 25 Slides
EUR 232

Mast Cell (Control Slides)

TCS0014-5 5 Slides
EUR 98

PAS/Glycogen (Control Slides)

TCS0017-100 100 Slides
EUR 706

PAS/Glycogen (Control Slides)

TCS0017-25 25 Slides
EUR 232

PAS/Glycogen (Control Slides)

TCS0017-5 5 Slides
EUR 98

Trichrome - Colon (Control Slides)

TCS0021-100 100 Slides
EUR 706

Utilities spray droplets in situ analysis and real-time monitoring of chemical reactions indicated by bis (cyclopentadienyl) zirconium dichloride (zirconocene dichloride) / methylaluminoxane, Cp2ZrCl2 / MAO, homogeneous ethylene polymerization catalyst in a variety of solutions. reaction time ranging from seconds to minutes, and the catalyst active species and products of ethylene polymers obtained and identified by tandem mass spectrometry.

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