A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.

A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.

In this paper we report the development of a modular, cost-effective open source, and fully automated slide-scanning microscope, composed entirely of readily available parts of the off-the-shelf, and capable of bright field and fluorescence mode.

Automatic X-Y stage consists of two steps Cheap micrometer coupled with the stepper motor is operated in open loop mode. The microscope consists of low cost CMOS sensor and lens’s board placed in the 4f configuration. This system has a resolution of about 1 micron, is limited by the f / # lens boards are available. The microscope is compact, measuring just 25 × 25 × 30 cm, and have the absolute positioning accuracy of ± 1 m in the X and the direction Y. A Z-phase imaging allows autofocus and a wider field of view even on non-planar samples, and custom software enables automatic determination of the boundaries of the sample and image mosaicking.

We demonstrate the utility of our devices through the blood imaging fluorescent- and transmission-dye stained and smears feces containing parasite of humans and animals, as well as some tissue samples ready. These results indicate that the image quality comparable to high-end commercial microscopes with a cost of less than US $ 400 for the bright-field system, with an additional US $ 100 is required for the fluorescence module.


In the current trend of miniaturization and simplification of imaging flow cytometry, Lab-on-a-Chip (LOC) of the microfluidic device represents an innovative and cost-effective solutions. Within this framework, we propose for the first time the platform novel based on the compactness of the microscope slide holographic (HMS) in combination with computing features the new space-time holography digital (STDH) using a 1D linear sensor array (LSA) is not a 2D CCD or CMOS camera to respond real diagnostic needs. In this LoC platform, computational methods, holography, and microfluidics interlinked to provide an imaging system by reducing the number of optical components and the ability to achieve a reliable cell counting even in the absence of highly accurate flow control. STDH utilize motion sample into a microfluidic channel to get the field-of-view is limited along the direction of flow, independent of magnification factor.

Furthermore, the typical numerical refocusing from modality hologram allows imaging and visualization of the entire volume of the channel, thus avoiding the loss of information due to the limited depth of focus of a standard microscope. As a result, we believe that this platform could open new perspectives to improve throughput by 3D volumetric imaging.

 A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.
A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.

Droplet spray ionization of a glass microscope slide: real-time monitoring of polymerization of ethylene.

Ambient ionization mass spectrometry is achieved in a simple manner by loading a sample solution onto a glass microscope cover corner is positioned in front of the inlet to the mass spectrometer and applying a high voltage to the sample. The resulting flow of charged droplets is stable, has no contamination from the substrate platform, and can be used repeatedly.

Microscope Slides Plain 1.0-1.2mm 76x52mm

MIC2156 PK50
EUR 15.73

Microscope slides, positive charged, 90° corners, frosted on one end, 25 x 75mm

M7100-90 72/pack
EUR 75.4
Description: Microscope slides positive charged

BRAND(TM) MICROSCOPE SLIDE CUT EDGES

BR474701-2500EA PK2500
EUR 175.67

Proteinase-resistant microscope slides

PO102 100 slides
EUR 236.4

Vinyl microscope slides RINZL

DD52951 PK144
EUR 24.62

Superfrost® Plus microscope slides

PO103 box (72 slides)
EUR 123.6

Slides 90o Ground Twin Frost 1mm Thick

MIC3806 PK50
EUR 8.66

Cardboard tray for 20 microscope slides

DD65205 EACH
EUR 15.85

Slides Cut Edge Plain 1mm Thick

MIC3800 PK50
EUR 3.99

Box for 25 microscope slides purple colour

DD49719 EACH
EUR 15.28

Box for 25 microscope slides orange colour

DD49720 EACH
EUR 15.28

Box for 25 microscope slides turquoise colour

DD49721 EACH
EUR 15.28

Gelatin-coated microscope slides (75 x 25 mm)

PO101 100 slides
EUR 236.4

Gelatin-coated microscope slides (75 x 50 mm)

PO105 100 slides
EUR 420

Polystyrene box for 100 microscope slides blue colour

DD139537 EACH
EUR 10.49

Polystyrene box for 100 microscope slides green colour

DD139538 EACH
EUR 10.49

Plastic box for 100 microscope slides (blue colour)

DD37501 EACH
EUR 29.64

Plastic box for 100 microscope slides (red colour)

DD37503 EACH
EUR 29.64

Plastic box for 50 microscope slides (blue colour)

DD37507 EACH
EUR 25.08

Premium Plain Glass Microscope Slides, 144 /pk, 1 mm thickness, 25 mm wide by 73 mm long

M-ACC-GSL9 200 TIPS
EUR 65
Description: Premium Plain Glass Microscope Slides, 144 /pk, 1 mm thickness, 25 mm wide by 73 mm long

Anti-Mouse Whole Serum | GWS-90

GWS-90 1.0 mL
EUR 145
Description:

Anti-Mouse Whole Serum | GWS-90 | Immunology Consultants Laboratory

Host: Goat

Format: Whole Serum

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Ground Joint Socket

8124/24/29 EACH
EUR 4.67

Plastic box for 100 microscope slides (assorted colours) cork bottom

DD10197 PK5
EUR 83.22

18 mm Circular Glass Cover Slides - Plain, 1000/CS

M-CSL18-103 1000 pcs.
EUR 218
Description: 18 mm Circular Glass Cover Slides - Plain, 1000/CS

22 mm Circular Glass Cover Slides - Plain, 1000/CS

M-CSL22-104 1000 pcs.
EUR 270
Description: 22 mm Circular Glass Cover Slides - Plain, 1000/CS

Bag Plain Seal 90 x 170 x 50mm

AUT1502 PK1000
EUR 85.5

Bag Plain Seal 90 x 250 x 50mm

AUT1504 PK1000
EUR 114

DiagNano Fluorophore Labeled Gold Nanoparticles, 90 nm

GFL-90 1 mL
EUR 1263.6

Turbidity Std Ratio 90 NTU

CRSR-90-100 100ML
EUR 125.4

Turbidity Std Ratio 90 NTU

CRSR-90-500 500ML
EUR 248.52

100-place microscope slide box (for 75 x 25 mm slides)

PO201 each
EUR 84

25-place microscope slide box (for 75 x 25 mm slides)

PO202 each
EUR 73.2

100-place microscope slide box (for 75 x 50 mm slides)

PO203 each
EUR 152.4

Changed orientation of crystal mounts to 90 degree angle,

CA-90 20 ADD ON PER BOX
EUR 54
Description: Changed orientation of crystal mounts to 90 degree angle, modification to 1 box of 20 mounts/loops/meshes(mounts ordered separately)

Electrode ground joint-junction

TIT3120 EACH
EUR 315.78

Turbidity Std Non Ratio 90 NTU

CRS-90-100 100ML
EUR 191.52

Turbidity Std Non Ratio 90 NTU

CRS-90-500 500ML
EUR 399

Normal Mouse Serum | RS-90-100ML

RS-90-100ML 100 mL
EUR 448
Description:

Normal Mouse Serum | RS-90-100ML | Immunology Consultants Laboratory

Species Reactivity: Mouse

Format: Serum

Product Type: Isotype Control

Antibody clonality: Polyclonal

Economical slide with cut edges

DD68763 PK50
EUR 5.7

Watch Glass 50mm Fused Edges

213213207 PK10
EUR 102.6

Watch Glass 60ml Fused Edges

2132134 PK10
EUR 123.12

Microscope slide dispenser for box of 72 standard 25 x 75mm slides

M7100-SD 1/pack
EUR 83.89
Description: Microscope slide dispenser

Microscope slides, positive charged, 45° corners, frosted on one end, 25 x 75mm

M7100-45 72/pack
EUR 75.4
Description: Microscope slides positive charged

Ground Sleeve Gland St20/2

QST20/2 EACH
EUR 118.51

Aspirator Pyrex Ground Side 5Ltr

ASP1060 EACH
EUR 172.62

Aspirator Pyrex Ground Side 10Lt

ASP1065 EACH
EUR 301.44

Aspirator Pyrex Ground Side 20Lt

ASP1070 EACH
EUR 485.66

Stereo Microscope Trinocular

MIC1590 EACH
EUR 1022.58

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 90 nm

GCK-M-90 1 kit
EUR 908.4

Positive control tissue section for each antibody; Based on availability INQUIRE

Control-Slides Set of 5
EUR 211.2

Quickfit Ground Socket Conical Tube 10ml

QBC24C14T0 PK10
EUR 211.26

Custom Peptide Synthesis (>90%; mass spec, hplc (mg-kg size, price based upon peptide size: 2-100 aa)

PEP-90 1 Ask for price

BA310 Elite Trinocular microscope

MIC2937 EACH
EUR 2139.78

Microscope Slide Polysine 75x25x1mm

MIC3050 PK72
EUR 34.66

BA210E Elite Binocular Microscope

MIC5633 EACH
EUR 1451.22

30ltr Plain Bin

EXBB304 PK10
EUR 74.1

6ltr Plain Bins

EXBBPIP64 PK40
EUR 101.46

Plain Glass Plates

VS10WPG PK2
EUR 69.54

ReadiUse™ microscope mounting solution

20009 50 mL
EUR 138

Digi-Pad Microscope Tablet Camera

MIC0115 EACH
EUR 1144.56

Motic BA210 LED Microscope Binocular

MIC2004 EACH
EUR 1337.22

Motic BA310 LED Microscope Binocular

MIC2016 EACH
EUR 1805.76

Motic BA310 LED Microscope Trinocular

MIC2018 EACH
EUR 2042.88

Motic SMZ-168-BL Microscope

MIC2800 EACH
EUR 1357.74

Microscope Slide Box True North

MIC4090 EACH
EUR 38.19

Amyloid (Control Slides)

TCS0003-100 100 Slides
EUR 847.2

Amyloid (Control Slides)

TCS0003-25 25 Slides
EUR 278.4

Amyloid (Control Slides)

TCS0003-5 5 Slides
EUR 117.6

Argentaffin (Control Slides)

TCS0004-100 100 Slides
EUR 847.2

Argentaffin (Control Slides)

TCS0004-25 25 Slides
EUR 278.4

Argentaffin (Control Slides)

TCS0004-5 5 Slides
EUR 117.6

Fungus (Control Slides)

TCS0005-100 100 Slides
EUR 847.2

Fungus (Control Slides)

TCS0005-25 25 Slides
EUR 278.4

Fungus (Control Slides)

TCS0005-5 5 Slides
EUR 117.6

Bielschowsky's (Control Slides)

TCS0006-100 100 Slides
EUR 847.2

Bielschowsky's (Control Slides)

TCS0006-25 25 Slides
EUR 278.4

Bielschowsky's (Control Slides)

TCS0006-5 5 Slides
EUR 117.6

Calcium (Control Slides)

TCS0007-100 100 Slides
EUR 847.2

Calcium (Control Slides)

TCS0007-25 25 Slides
EUR 278.4

Calcium (Control Slides)

TCS0007-5 5 Slides
EUR 117.6

Elastic (Control Slides)

TCS0008-100 100 Slides
EUR 847.2

Elastic (Control Slides)

TCS0008-25 25 Slides
EUR 278.4

Elastic (Control Slides)

TCS0008-5 5 Slides
EUR 117.6

Melanin (Control Slides)

TCS0015-100 100 Slides
EUR 847.2

Melanin (Control Slides)

TCS0015-25 25 Slides
EUR 278.4

Melanin (Control Slides)

TCS0015-5 5 Slides
EUR 117.6

Mucin (Control Slides)

TCS0016-100 100 Slides
EUR 847.2

Mucin (Control Slides)

TCS0016-25 25 Slides
EUR 278.4

Mucin (Control Slides)

TCS0016-5 5 Slides
EUR 117.6

Reticulum (Control Slides)

TCS0019-100 100 Slides
EUR 847.2

Reticulum (Control Slides)

TCS0019-25 25 Slides
EUR 278.4

Reticulum (Control Slides)

TCS0019-5 5 Slides
EUR 117.6

Funnel Plain PP 35mm

FUN2088 PK36
EUR 16.76

Funnel Plain PP 50mm

FUN2090 PK36
EUR 18.58

Funnel Plain PP 55mm

FUN2092 PK36
EUR 18.58

Funnel Plain PP 65mm

FUN2094 PK12
EUR 10.6

Funnel Plain PP 75mm

FUN2096 PK36
EUR 32.26

Funnel Plain PP 90mm

FUN2098 PK12
EUR 18.58

Funnel Plain PP 100mm

FUN21020 PK12
EUR 22

Sterilin Certified Tubes - Plain

CON7496 PK400
EUR 231.42

Techne Plain Block Insert

BLO2100 EACH
EUR 139.08

Techne Block Plain Thermometer

BLO2120 EACH
EUR 401.28

Techne Block Plain Thermometer

BLO2122 EACH
EUR 141.36

20mm Alum CrimpCap plain

CHR2112 PK1000
EUR 41.04

20mm Alum CrimpCap plain

CHR3158 PK1000
EUR 622.44

Bottle Trigger Spray Plain

CLE9010P EACH
EUR 7.18

PIK-90

E1KS1187 5mg
EUR 390

PIK-90

A2764-10 10 mg
EUR 174
Description: PIK-90 is a broad-spectrum PI3K inhibitors that inhibits PI3K?, PI3K? and PI3K? with IC50 values of 11, 18 and 58nM, respectively [1].Studies showed that PIK-90 induced increased levels of secreted IgE at 1 ?mol/L, while it inhibited IgE production at doses of greater than 2 ?mol/L [2].

PIK-90

A2764-5 5 mg
EUR 141.6
Description: PIK-90 is a broad-spectrum PI3K inhibitors that inhibits PI3K?, PI3K? and PI3K? with IC50 values of 11, 18 and 58nM, respectively [1].Studies showed that PIK-90 induced increased levels of secreted IgE at 1 ?mol/L, while it inhibited IgE production at doses of greater than 2 ?mol/L [2].

PIK-90

B1671-25
EUR 582

PIK-90

B1671-5
EUR 189.6

IMR-90

P0016019 One Frozen vial
EUR 546

LR-90

HY-76383 10mM/1mL
EUR 596.4

PIK-90

HY-12030 5mg
EUR 129.6

Microscope Slide Boxes,100-Place, Blue

LC7002-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, Blue

LC7002-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, Blue

LC7002-100 100/CS
EUR 967.2

Microscope Slide Boxes,100-Place, Green

LC7003-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, Green

LC7003-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, Green

LC7003-100 100/CS
EUR 967.2

Microscope Slide Boxes,100-Place, Red

LC7004-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, Red

LC7004-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, Red

LC7004-100 100/CS
EUR 967.2

Microscope Slide Boxes,100-Place, Yellow

LC7005-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, Yellow

LC7005-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, Yellow

LC7005-100 100/CS
EUR 967.2

Microscope Slide Boxes,100-Place, White

LC7006-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, White

LC7006-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, White

LC7006-100 100/CS
EUR 967.2

Microscope Slide Boxes,25-Place, Blue

LC7007-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Blue

LC7007-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Blue

LC7007-100 100/CS
EUR 684

Microscope Slide Boxes,25-Place, Green

LC7008-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Green

LC7008-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Green

LC7008-100 100/CS
EUR 684

Microscope Slide Boxes,25-Place, Red

LC7009-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Red

LC7009-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Red

LC7009-100 100/CS
EUR 684

Microscope Slide Boxes,25-Place, Yellow

LC7010-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Yellow

LC7010-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Yellow

LC7010-100 100/CS
EUR 684

Microscope Slide Boxes,25-Place, White

LC7011-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, White

LC7011-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, White

LC7011-100 100/CS
EUR 684

Microscope Binoc MAGNUM-B planachrom obj

MIC0105 EACH
EUR 1800.06

Microscope Trinoc MAGNUM-T planachrom obj

MIC0107 EACH
EUR 1868.46

Microscope Pack 1 CX23 100x Oil

MIC1968 EACH
EUR 1827.42

Microscope Pack 2 CX23 BIN LED

MIC1970 EACH
EUR 1447.8

Microscope Cover Slips No. 0 18x18mm

MIC2158 PK100
EUR 2.28

Microscope Cover Slips No. 0 22x22mm

MIC2160 PK100
EUR 2.96

Microscope Cover Slips No. 1 18x18mm

MIC2162 PK100
EUR 1.94

Microscope Cover Slips No. 1 22x22mm

MIC2164 PK100
EUR 2.62

Microscope Cover Slips No. 1.5 18x18mm

MIC2166 PK100
EUR 2.05

Microscope Cover Slips No. 1.5 22x22mm

MIC2168 PK100
EUR 2.96

Microscope Cover Slips No.1.5 22x26mm

MIC2170 PK100
EUR 3.53

Microscope Cover Slips No. 1.5 22x32mm

MIC2172 PK100
EUR 4.1

Microscope Cover Slips No. 1.5 22x40mm

MIC2174 PK100
EUR 4.9

Microscope Cover Slips No. 2 18x18mm

MIC2176 PK100
EUR 2.39

Microscope Cover Slips No. 2 22x22mm

MIC2178 PK100
EUR 3.31

Microscope Cover Slips No. 1.5 16mm

MIC2182 PK100
EUR 8.89

Microscope Slide Box 100 Place Tall

MIC4080 EACH
EUR 38.19

Microscope Slide Box 25 ULT safe

MIC4092 EACH
EUR 17.62

SMZ-171-Binocular LED Stero Microscope

MIC5444 EACH
EUR 1596

SMZ-171-Trinocular LED Stero Microscope

MIC5445 EACH
EUR 1691.76

SMZ-171-Binocular P Stero Microscope

MIC5446 EACH
EUR 1244.88

Utilities spray droplets in situ analysis and real-time monitoring of chemical reactions indicated by bis (cyclopentadienyl) zirconium dichloride (zirconocene dichloride) / methylaluminoxane, Cp2ZrCl2 / MAO, homogeneous ethylene polymerization catalyst in a variety of solutions. reaction time ranging from seconds to minutes, and the catalyst active species and products of ethylene polymers obtained and identified by tandem mass spectrometry.

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