The influence of the microscope lamp filament colour temperature on the process of digital images of histological slides acquisition standardization.

The influence of the microscope lamp filament colour temperature on the process of digital images of histological slides acquisition standardization.

BACKGROUND
The purpose of this study was to compare the digital image of the biopsy tissue taken with an optical microscope using bright field techniques under various lighting conditions. Various colors in immunohistochemistry stained with 3,3′-diaminobenzidine and Haematoxylin very large and tissue samples derived from various sources. One of them is the inadequate arrangements camera’s white balance color temperature of the light microscope.

Although these types of errors can be easily handled during the image acquisition phase, can be eliminated by using a color adjustment algorithms. Examination of the color variation of the temperature dependence of light microscope and camera settings is done as a preliminary study for automatic color standardization process.


METHOD
Six field of view with an empty space between these tissue samples selected for analysis. Each field of view has acquired 225 times with mild temperatures variety of microscopes and camera white balance settings. Fourteen of randomly selected images has been improved and compared with reference images, by the following methods: Mean Square Error, Structural similarity and a visual assessment viewer.


RESULTS
For the two types of background and two types of objects, descriptors statistical picture: the range, median, mean and standard deviation of the chromaticity on the A and B channels of the CIELAB color space, and lighting L, and the variability of local color to a specific area objects have counted. The result has been the average over the six images obtained in the same light conditions and camera settings for each sample.


CONCLUSION
Analysis of lead results following conclusions: (1) the images collected with the white balance adjusted for cluster color temperature of light in certain regions of space chromatic, (2) the process of correction of white balance to the images collected by the camera white balance settings are not suitable for the temperature light the explanatory moving picture to the right color space but at the same time value the lighting changes. So the unification process in terms of color fidelity image can be completed in a separate introductory stage before automatic image analysis.

 The influence of the microscope lamp filament colour temperature on the process of digital images of histological slides acquisition standardization.
The influence of the microscope lamp filament colour temperature on the process of digital images of histological slides acquisition standardization.

centrosome research historical roots: the invention of the microscope slide Boveri in Würzburg.

Boveri visionary monograph ‘Ueber die Natur der Centrosomen’ (On the nature of centrosomes) in 1900 founded largely on microscopic observations of sea urchin egg divides and Ascaris roundworm parasites.

As Boveri wrote in the introductory paragraph, less importance on morphological aspects of centrosomes, but aimed at an understanding of their physiological roles during cell division. The remarkable transition from observations of small dot-like structure remains and pieces of material for a unified theory of centrosome function (which is basically still true today) can not be fully appreciated without checking this starting material Boveri, histological specimens.

Microscope slides, positive charged, 90° corners, frosted on one end, 25 x 75mm

M7100-90 72/pack
EUR 75.4
Description: Microscope slides positive charged

Superfrost® Plus microscope slides

PO103 box (72 slides)
EUR 123.6

Gold Seal Rite-On® frosted slides

PO104 box (72 slides)
EUR 108

SLS Slides Single Cavity

MIC3450 PK50
EUR 21.75

Microscope Slides Plain 1.2-1.5mm 76x26mm

MIC2154 PK50
EUR 3.88

Microscope Slides Plain 1.0-1.2mm 76x52mm

MIC2156 PK50
EUR 15.73

Cardboard tray for 20 microscope slides

DD65205 EACH
EUR 15.85

Micro Slides Tropical Frosted One End One Side

MIC1008 PK50
EUR 2.51

Box for 25 microscope slides purple colour

DD49719 EACH
EUR 15.28

Box for 25 microscope slides orange colour

DD49720 EACH
EUR 15.28

Box for 25 microscope slides turquoise colour

DD49721 EACH
EUR 15.28

Gelatin-coated microscope slides (75 x 25 mm)

PO101 100 slides
EUR 236.4

Gelatin-coated microscope slides (75 x 50 mm)

PO105 100 slides
EUR 420

Polystyrene box for 100 microscope slides blue colour

DD139537 EACH
EUR 10.49

Polystyrene box for 100 microscope slides green colour

DD139538 EACH
EUR 10.49

Plastic box for 100 microscope slides (blue colour)

DD37501 EACH
EUR 29.64

Plastic box for 100 microscope slides (red colour)

DD37503 EACH
EUR 29.64

Plastic box for 50 microscope slides (blue colour)

DD37507 EACH
EUR 25.08

Plastic box for 100 microscope slides (assorted colours) cork bottom

DD10197 PK5
EUR 83.22

100-place microscope slide box (for 75 x 25 mm slides)

PO201 each
EUR 84

25-place microscope slide box (for 75 x 25 mm slides)

PO202 each
EUR 73.2

100-place microscope slide box (for 75 x 50 mm slides)

PO203 each
EUR 152.4

Watch Glass 50mm Fused Edges

213213207 PK10
EUR 102.6

Watch Glass 60ml Fused Edges

2132134 PK10
EUR 123.12

Economical slide with cut edges

DD68763 PK50
EUR 5.7

Microscope slide dispenser for box of 72 standard 25 x 75mm slides

M7100-SD 1/pack
EUR 83.89
Description: Microscope slide dispenser

Bovine enterovirus (BEV) PCR kit

PCR-V728-48D 50T
EUR 543.6

Bovine enterovirus (BEV) PCR kit

PCR-V728-96D 100T
EUR 686.4

96 Semi Skirted Frosted Wells

PCR1120 PK50
EUR 250.8

96 Semi Skirted Frosted Wells

PCR1122 PK10
EUR 78.66

Economical slide with ground edges 90

DD68765 PK50
EUR 7.98

Stereo Microscope Trinocular

MIC1590 EACH
EUR 1022.58

Bovine enterovirus (BEV) RT PCR kit

RTq-V728-100D 100T
EUR 860.4

Bovine enterovirus (BEV) RT PCR kit

RTq-V728-150D 150T
EUR 969.6

Bovine enterovirus (BEV) RT PCR kit

RTq-V728-50R 50T
EUR 1155.6

Positive control tissue section for each antibody; Based on availability INQUIRE

Control-Slides Set of 5
EUR 211.2

BA310 Elite Trinocular microscope

MIC2937 EACH
EUR 2139.78

Microscope Slide Polysine 75x25x1mm

MIC3050 PK72
EUR 34.66

BA210E Elite Binocular Microscope

MIC5633 EACH
EUR 1451.22

Bovine enterovirus (BEV) One-Step PCR kit

Oneq-V728-100D 100T
EUR 1039.2

Bovine enterovirus (BEV) One-Step PCR kit

Oneq-V728-150D 150T
EUR 1177.2

Bovine enterovirus (BEV) One-Step PCR kit

Oneq-V728-50D 50T
EUR 861.6

Premium Plain Glass Microscope Slides, 144 /pk, 1 mm thickness, 25 mm wide by 73 mm long

M-ACC-GSL9 200 TIPS
EUR 65
Description: Premium Plain Glass Microscope Slides, 144 /pk, 1 mm thickness, 25 mm wide by 73 mm long

ReadiUse™ microscope mounting solution

20009 50 mL
EUR 138

Digi-Pad Microscope Tablet Camera

MIC0115 EACH
EUR 1144.56

Motic BA210 LED Microscope Binocular

MIC2004 EACH
EUR 1337.22

Motic BA310 LED Microscope Binocular

MIC2016 EACH
EUR 1805.76

Motic BA310 LED Microscope Trinocular

MIC2018 EACH
EUR 2042.88

Motic SMZ-168-BL Microscope

MIC2800 EACH
EUR 1357.74

Microscope Slide Box True North

MIC4090 EACH
EUR 38.19

Amyloid (Control Slides)

TCS0003-100 100 Slides
EUR 847.2

Amyloid (Control Slides)

TCS0003-25 25 Slides
EUR 278.4

Amyloid (Control Slides)

TCS0003-5 5 Slides
EUR 117.6

Argentaffin (Control Slides)

TCS0004-100 100 Slides
EUR 847.2

Argentaffin (Control Slides)

TCS0004-25 25 Slides
EUR 278.4

Argentaffin (Control Slides)

TCS0004-5 5 Slides
EUR 117.6

Fungus (Control Slides)

TCS0005-100 100 Slides
EUR 847.2

Fungus (Control Slides)

TCS0005-25 25 Slides
EUR 278.4

Fungus (Control Slides)

TCS0005-5 5 Slides
EUR 117.6

Bielschowsky's (Control Slides)

TCS0006-100 100 Slides
EUR 847.2

Bielschowsky's (Control Slides)

TCS0006-25 25 Slides
EUR 278.4

Bielschowsky's (Control Slides)

TCS0006-5 5 Slides
EUR 117.6

Calcium (Control Slides)

TCS0007-100 100 Slides
EUR 847.2

Calcium (Control Slides)

TCS0007-25 25 Slides
EUR 278.4

Calcium (Control Slides)

TCS0007-5 5 Slides
EUR 117.6

Elastic (Control Slides)

TCS0008-100 100 Slides
EUR 847.2

Elastic (Control Slides)

TCS0008-25 25 Slides
EUR 278.4

Elastic (Control Slides)

TCS0008-5 5 Slides
EUR 117.6

Melanin (Control Slides)

TCS0015-100 100 Slides
EUR 847.2

Melanin (Control Slides)

TCS0015-25 25 Slides
EUR 278.4

Melanin (Control Slides)

TCS0015-5 5 Slides
EUR 117.6

Mucin (Control Slides)

TCS0016-100 100 Slides
EUR 847.2

Mucin (Control Slides)

TCS0016-25 25 Slides
EUR 278.4

Mucin (Control Slides)

TCS0016-5 5 Slides
EUR 117.6

Reticulum (Control Slides)

TCS0019-100 100 Slides
EUR 847.2

Reticulum (Control Slides)

TCS0019-25 25 Slides
EUR 278.4

Reticulum (Control Slides)

TCS0019-5 5 Slides
EUR 117.6

Microscope Slide Boxes,100-Place, Blue

LC7002-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, Blue

LC7002-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, Blue

LC7002-100 100/CS
EUR 967.2

Microscope Slide Boxes,100-Place, Green

LC7003-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, Green

LC7003-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, Green

LC7003-100 100/CS
EUR 967.2

Microscope Slide Boxes,100-Place, Red

LC7004-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, Red

LC7004-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, Red

LC7004-100 100/CS
EUR 967.2

Microscope Slide Boxes,100-Place, Yellow

LC7005-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, Yellow

LC7005-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, Yellow

LC7005-100 100/CS
EUR 967.2

Microscope Slide Boxes,100-Place, White

LC7006-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, White

LC7006-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, White

LC7006-100 100/CS
EUR 967.2

Microscope Slide Boxes,25-Place, Blue

LC7007-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Blue

LC7007-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Blue

LC7007-100 100/CS
EUR 684

Microscope Slide Boxes,25-Place, Green

LC7008-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Green

LC7008-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Green

LC7008-100 100/CS
EUR 684

Microscope Slide Boxes,25-Place, Red

LC7009-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Red

LC7009-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Red

LC7009-100 100/CS
EUR 684

Microscope Slide Boxes,25-Place, Yellow

LC7010-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Yellow

LC7010-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Yellow

LC7010-100 100/CS
EUR 684

Microscope Slide Boxes,25-Place, White

LC7011-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, White

LC7011-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, White

LC7011-100 100/CS
EUR 684

VIKEM(R) VINYL MICROSCOPE COVER EXTRA

BAF243040000-1EA EACH
EUR 32.38

Microscope Binoc MAGNUM-B planachrom obj

MIC0105 EACH
EUR 1800.06

Microscope Trinoc MAGNUM-T planachrom obj

MIC0107 EACH
EUR 1868.46

Microscope Pack 1 CX23 100x Oil

MIC1968 EACH
EUR 1827.42

Microscope Pack 2 CX23 BIN LED

MIC1970 EACH
EUR 1447.8

Microscope Cover Slips No. 0 18x18mm

MIC2158 PK100
EUR 2.28

Microscope Cover Slips No. 0 22x22mm

MIC2160 PK100
EUR 2.96

Microscope Cover Slips No. 1 18x18mm

MIC2162 PK100
EUR 1.94

Microscope Cover Slips No. 1 22x22mm

MIC2164 PK100
EUR 2.62

Microscope Cover Slips No. 1.5 18x18mm

MIC2166 PK100
EUR 2.05

Microscope Cover Slips No. 1.5 22x22mm

MIC2168 PK100
EUR 2.96

Microscope Cover Slips No.1.5 22x26mm

MIC2170 PK100
EUR 3.53

Microscope Cover Slips No. 1.5 22x32mm

MIC2172 PK100
EUR 4.1

Microscope Cover Slips No. 1.5 22x40mm

MIC2174 PK100
EUR 4.9

Microscope Cover Slips No. 2 18x18mm

MIC2176 PK100
EUR 2.39

Microscope Cover Slips No. 2 22x22mm

MIC2178 PK100
EUR 3.31

Microscope Cover Slips No. 1.5 16mm

MIC2182 PK100
EUR 8.89

Microscope Slide Box 100 Place Tall

MIC4080 EACH
EUR 38.19

Microscope Slide Box 25 ULT safe

MIC4092 EACH
EUR 17.62

SMZ-171-Binocular LED Stero Microscope

MIC5444 EACH
EUR 1596

SMZ-171-Trinocular LED Stero Microscope

MIC5445 EACH
EUR 1691.76

SMZ-171-Binocular P Stero Microscope

MIC5446 EACH
EUR 1244.88

SMZ-171-Trinocular P Stero Microscope

MIC5447 EACH
EUR 1338.36

Motic SMZ-140-N2GG Stereo Microscope

MIC5900 EACH
EUR 621.3

Motic SMZ-143-N2GG Stereo Microscope

MIC5902 EACH
EUR 722.76

MIGHTY SCOPE(TM) DIGITAL MICROSCOPE 5M

Z742668-1EA EACH
EUR 348.04

CometAssay High Throughput Slides

4252-02K-01 100 tests
EUR 1943.28

CometAssay Alkaline Control Slides

4256-010-CC 10 Tests
EUR 389.28

CometAssay Neutral Control Slides

4257-010-NC 10 Tests
EUR 389.28

Alcian Blue (Control Slides)

TCS0002-100 100 Slides
EUR 847.2

Alcian Blue (Control Slides)

TCS0002-25 25 Slides
EUR 278.4

Alcian Blue (Control Slides)

TCS0002-5 5 Slides
EUR 117.6

Helicobacter Pylori (Control Slides)

TCS0011-100 100 Slides
EUR 847.2

Helicobacter Pylori (Control Slides)

TCS0011-25 25 Slides
EUR 278.4

Helicobacter Pylori (Control Slides)

TCS0011-5 5 Slides
EUR 117.6

Iron - Liver (Control Slides)

TCS0013-100 100 Slides
EUR 847.2

Iron - Liver (Control Slides)

TCS0013-25 25 Slides
EUR 278.4

Iron - Liver (Control Slides)

TCS0013-5 5 Slides
EUR 117.6

Mast Cell (Control Slides)

TCS0014-100 100 Slides
EUR 847.2

Mast Cell (Control Slides)

TCS0014-25 25 Slides
EUR 278.4

Mast Cell (Control Slides)

TCS0014-5 5 Slides
EUR 117.6

PAS/Glycogen (Control Slides)

TCS0017-100 100 Slides
EUR 847.2

PAS/Glycogen (Control Slides)

TCS0017-25 25 Slides
EUR 278.4

PAS/Glycogen (Control Slides)

TCS0017-5 5 Slides
EUR 117.6

Trichrome - Colon (Control Slides)

TCS0021-100 100 Slides
EUR 847.2

Trichrome - Colon (Control Slides)

TCS0021-25 25 Slides
EUR 278.4

Trichrome - Colon (Control Slides)

TCS0021-5 5 Slides
EUR 117.6

Trichrome - Liver (Control Slides)

TCS0022-100 100 Slides
EUR 847.2

Trichrome - Liver (Control Slides)

TCS0022-25 25 Slides
EUR 278.4

Trichrome - Liver (Control Slides)

TCS0022-5 5 Slides
EUR 117.6

TOMO Slides 1 Box

TOMO100 100 Slides
EUR 108

Slides Economy 76x26 1mm

MIC2000 PK50
EUR 4.56

Slides Superfrost 76X26mm White

MIC3020 PK100
EUR 17.33

Slides Superfrost 76X26mm Pnk

MIC3026 PK100
EUR 17.33

Slides Superfrost 76X26mm Yell

MIC3028 PK100
EUR 16.5

Slides Superfrost 76X26mm Orng

MIC3030 PK100
EUR 17.9

Insert For 210 Slides

WAS6098 EACH
EUR 409.26

Single Edge Blades

INS4510 PK100
EUR 39.1

Spreader single wrap

SLS1999 PK250
EUR 49.93

It is generally assumed that the microscope slides are lost during the bombing of the Zoological Institute in Würzburg at the end of World War II. Here, I describe the discovery of a number of original microscope slides Boveri with early serial section and Ascaris sea urchin embryo, stained with hematoxylin iron HEIDENHAIN this method. Some slides bear handwritten notes and sketches by Boveri. Evidence is presented that the new slide is found is part of the original materials used by Boveri’s seminal monograph centrosome.

Leave A Comment