BACKGROUND
The purpose of this study was to compare the digital image of the biopsy tissue taken with an optical microscope using bright field techniques under various lighting conditions. Various colors in immunohistochemistry stained with 3,3′-diaminobenzidine and Haematoxylin very large and tissue samples derived from various sources. One of them is the inadequate arrangements camera’s white balance color temperature of the light microscope.

Although these types of errors can be easily handled during the image acquisition phase, can be eliminated by using a color adjustment algorithms. Examination of the color variation of the temperature dependence of light microscope and camera settings is done as a preliminary study for automatic color standardization process.

METHOD
Six field of view with an empty space between these tissue samples selected for analysis. Each field of view has acquired 225 times with mild temperatures variety of microscopes and camera white balance settings. Fourteen of randomly selected images has been improved and compared with reference images, by the following methods: Mean Square Error, Structural similarity and a visual assessment viewer.

RESULTS
For the two types of background and two types of objects, descriptors statistical picture: the range, median, mean and standard deviation of the chromaticity on the A and B channels of the CIELAB color space, and lighting L, and the variability of local color to a specific area objects have counted. The result has been the average over the six images obtained in the same light conditions and camera settings for each sample.

CONCLUSION
Analysis of lead results following conclusions: (1) the images collected with the white balance adjusted for cluster color temperature of light in certain regions of space chromatic, (2) the process of correction of white balance to the images collected by the camera white balance settings are not suitable for the temperature light the explanatory moving picture to the right color space but at the same time value the lighting changes. So the unification process in terms of color fidelity image can be completed in a separate introductory stage before automatic image analysis.

centrosome research historical roots: the invention of the microscope slide Boveri in Würzburg.

Boveri visionary monograph ‘Ueber die Natur der Centrosomen’ (On the nature of centrosomes) in 1900 founded largely on microscopic observations of sea urchin egg divides and Ascaris roundworm parasites.

As Boveri wrote in the introductory paragraph, less importance on morphological aspects of centrosomes, but aimed at an understanding of their physiological roles during cell division. The remarkable transition from observations of small dot-like structure remains and pieces of material for a unified theory of centrosome function (which is basically still true today) can not be fully appreciated without checking this starting material Boveri, histological specimens.

Vinyl microscope slides RINZL
DD52951	Scientific Laboratory Supplies	PK144	EUR 24.62

Superfrost® Plus microscope slides
PO103	FD Neurotechnologies	box (72 slides)	EUR 123.6

Gold Seal Rite-On® frosted slides
PO104	FD Neurotechnologies	box (72 slides)	EUR 108

Microscope Slides Plain 1.2-1.5mm 76x26mm
MIC2154	Scientific Laboratory Supplies	PK50	EUR 3.88

Microscope Slides Plain 1.0-1.2mm 76x52mm
MIC2156	Scientific Laboratory Supplies	PK50	EUR 15.73

Cardboard tray for 20 microscope slides
DD65205	Scientific Laboratory Supplies	EACH	EUR 15.85

SLS Slides Single Cavity
MIC3450	Scientific Laboratory Supplies	PK50	EUR 21.75

Micro Slides Tropical Frosted One End One Side
MIC1008	Scientific Laboratory Supplies	PK50	EUR 2.51

Box for 25 microscope slides purple colour
DD49719	Scientific Laboratory Supplies	EACH	EUR 15.28

Box for 25 microscope slides orange colour
DD49720	Scientific Laboratory Supplies	EACH	EUR 15.28

Box for 25 microscope slides turquoise colour
DD49721	Scientific Laboratory Supplies	EACH	EUR 15.28

Gelatin-coated microscope slides (75 x 25 mm)
PO101	FD Neurotechnologies	100 slides	EUR 236.4

Gelatin-coated microscope slides (75 x 50 mm)
PO105	FD Neurotechnologies	100 slides	EUR 420

Polystyrene box for 100 microscope slides blue colour
DD139537	Scientific Laboratory Supplies	EACH	EUR 10.49

Polystyrene box for 100 microscope slides green colour
DD139538	Scientific Laboratory Supplies	EACH	EUR 10.49

Plastic box for 100 microscope slides (blue colour)
DD37501	Scientific Laboratory Supplies	EACH	EUR 29.64

Plastic box for 100 microscope slides (red colour)
DD37503	Scientific Laboratory Supplies	EACH	EUR 29.64

Plastic box for 50 microscope slides (blue colour)
DD37507	Scientific Laboratory Supplies	EACH	EUR 25.08

Plastic box for 100 microscope slides (assorted colours) cork bottom
DD10197	Scientific Laboratory Supplies	PK5	EUR 83.22

100-place microscope slide box (for 75 x 25 mm slides)
PO201	FD Neurotechnologies	each	EUR 84

25-place microscope slide box (for 75 x 25 mm slides)
PO202	FD Neurotechnologies	each	EUR 73.2

100-place microscope slide box (for 75 x 50 mm slides)
PO203	FD Neurotechnologies	each	EUR 152.4

Economical slide with cut edges
DD68763	Scientific Laboratory Supplies	PK50	EUR 5.7

Watch Glass 50mm Fused Edges
213213207	Scientific Laboratory Supplies	PK10	EUR 102.6

Watch Glass 60ml Fused Edges
2132134	Scientific Laboratory Supplies	PK10	EUR 123.12

Cynomolgus Monkey:Placenta (single section per slide) (5 slides/pack)
CyFPT202	TissueArray	5 slides	EUR 150

Cynomolgus Monkey:Cartilage (single section per slide) (5 slides/pack)
CyFPT203	TissueArray	5 slides	EUR 150

Human Retinoblastoma (single section per slide) (5 slides/pack)
HuCAT601	TissueArray	5 slides	EUR 150

Human Retinoblastoma (single section per slide) (5 slides/pack)
HuCAT602	TissueArray	5 slides	EUR 150

Malignant mesothelioma (single section per slide) (5 slides/pack)
HuCAT605	TissueArray	5 slides	EUR 180

Ulcerative colitis (single section per slide) (5 slides/pack)
HuDAT032	TissueArray	5 slides	EUR 270

Gastric ulcer (single section per slide) (5 slides/pack)
HuDAT036	TissueArray	5 slides	EUR 270

Eosinophilic granuloma (single section per slide) (5 slides/pack)
HuDAT037	TissueArray	5 slides	EUR 270

Chronic tonsillitis (single section per slide) (5 slides/pack)
HuDAT188	TissueArray	5 slides	EUR 180

Combined nevus (single section per slide) (5 slides/pack)
HuDAT201	TissueArray	5 slides	EUR 270

Normal brain (single section per slide) (5 slides/pack)
HuFPT017	TissueArray	5 slides	EUR 150

Normal brain (single section per slide) (5 slides/pack)
HuFPT018	TissueArray	5 slides	EUR 150

Normal brain (single section per slide) (5 slides/pack)
HuFPT019	TissueArray	5 slides	EUR 150

Normal brain (single section per slide) (5 slides/pack)
HuFPT020	TissueArray	5 slides	EUR 150

Normal Brain (single section per slide) (5 slides/pack)
HuFPT021	TissueArray	5 slides	EUR 180

Normal Lung (single section per slide) (5 slides/pack)
HuFPT178	TissueArray	5 slides	EUR 150

Rat Normal (single section per slide) (5 slides/pack)
RaFPT096	TissueArray	5 slides	EUR 150

Bovine enterovirus (BEV) PCR kit
PCR-V728-48D	Bioingentech	50T	EUR 543.6

Bovine enterovirus (BEV) PCR kit
PCR-V728-96D	Bioingentech	100T	EUR 686.4

Microscope slide dispenser for box of 72 standard 25 x 75mm slides
M7100-SD	MTC Bio	1/pack	EUR 83.89
Description: Microscope slide dispenser

96 Semi Skirted Frosted Wells
PCR1120	Scientific Laboratory Supplies	PK50	EUR 250.8

96 Semi Skirted Frosted Wells
PCR1122	Scientific Laboratory Supplies	PK10	EUR 78.66

Economical slide with ground edges 90
DD68765	Scientific Laboratory Supplies	PK50	EUR 7.98

Cynomolgus Monkey: Heart (single section per slide) (5 slides/pack)
CyFPT001	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Pericardium (single section per slide) (5 slides/pack)
CyFPT002	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Kidney (single section per slide) (5 slides/pack)
CyFPT006	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Rectum (single section per slide) (5 slides/pack)
CyFPT021	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Gallbladder (single section per slide) (5 slides/pack)
CyFPT026	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Skin (single section per slide) (5 slides/pack)
CyFPT036	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Cerebrum (single section per slide) (5 slides/pack)
CyFPT046	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Pontine (single section per slide) (5 slides/pack)
CyFPT051	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Cerebellum (single section per slide) (5 slides/pack)
CyFPT061	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Spleen (single section per slide) (5 slides/pack)
CyFPT071	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Uterus (single section per slide) (5 slides/pack)
CyFPT081	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Cunnus (single section per slide) (5 slides/pack)
CyFPT087	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Esophagus (single section per slide) (5 slides/pack)
CyFPT091	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Colon (single section per slide) (5 slides/pack)
CyFPT096	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Stomach (single section per slide) (5 slides/pack)
CyFPT101	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Liver (single section per slide) (5 slides/pack)
CyFPT111	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Lung (single section per slide) (5 slides/pack)
CyFPT136	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Thymus (single section per slide) (5 slides/pack)
CyFPT141	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Tonsil (single section per slide) (5 slides/pack)
CyFPT146	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Bladder (single section per slide) (5 slides/pack)
CyFPT156	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Trachea (single section per slide) (5 slides/pack)
CyFPT161	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Prostate (single section per slide) (5 slides/pack)
CyFPT171	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Testis (single section per slide) (5 slides/pack)
CyFPT181	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Ureter (single section per slide) (5 slides/pack)
CyFPT186	TissueArray	5 slides	EUR 150

Cynomolgus Monkey: Penis (single section per slide) (5 slides/pack)
CyFPT191	TissueArray	5 slides	EUR 150

Human Brain Cancer (single section per slide) (5 slides/pack)
HuCAT001	TissueArray	5 slides	EUR 150

Human Brain Astrocytoma (single section per slide) (5 slides/pack)
HuCAT002	TissueArray	5 slides	EUR 150

Human Brain Cancer (single section per slide) (5 slides/pack)
HuCAT006	TissueArray	5 slides	EUR 150

Human Brain Cancer (single section per slide) (5 slides/pack)
HuCAT011	TissueArray	5 slides	EUR 150

Human Brain Cancer (single section per slide) (5 slides/pack)
HuCAT016	TissueArray	5 slides	EUR 150

Human Brain Cancer (single section per slide) (5 slides/pack)
HuCAT017	TissueArray	5 slides	EUR 150

Human Brain Cancer (single section per slide) (5 slides/pack)
HuCAT019	TissueArray	5 slides	EUR 150

Human Brain Cancer (single section per slide) (5 slides/pack)
HuCAT020	TissueArray	5 slides	EUR 150

Human Brain Cancer (single section per slide) (5 slides/pack)
HuCAT021	TissueArray	5 slides	EUR 150

Human Esophagus Cancer (single section per slide) (5 slides/pack)
HuCAT026	TissueArray	5 slides	EUR 150

Human Esophagus Cancer (single section per slide) (5 slides/pack)
HuCAT031	TissueArray	5 slides	EUR 150

Human Esophagus Cancer (single section per slide) (5 slides/pack)
HuCAT036	TissueArray	5 slides	EUR 150

Human Esophagus Cancer (single section per slide) (5 slides/pack)
HuCAT041	TissueArray	5 slides	EUR 150

Human Stomach Cancer (single section per slide) (5 slides/pack)
HuCAT046	TissueArray	5 slides	EUR 150

Human Stomach Cancer (single section per slide) (5 slides/pack)
HuCAT051	TissueArray	5 slides	EUR 150

Human Stomach Cancer (single section per slide) (5 slides/pack)
HuCAT056	TissueArray	5 slides	EUR 150

Human Stomach Cancer (single section per slide) (5 slides/pack)
HuCAT061	TissueArray	5 slides	EUR 150

Human Stomach Cancer (single section per slide) (5 slides/pack)
HuCAT066	TissueArray	5 slides	EUR 150

Human Liver Cancer (single section per slide) (5 slides/pack)
HuCAT071	TissueArray	5 slides	EUR 150

Human Liver Cancer (single section per slide) (5 slides/pack)
HuCAT076	TissueArray	5 slides	EUR 150

Human Liver Cancer (single section per slide) (5 slides/pack)
HuCAT081	TissueArray	5 slides	EUR 150

Human Liver Cancer (single section per slide) (5 slides/pack)
HuCAT086	TissueArray	5 slides	EUR 150

Human Colon Cancer (single section per slide) (5 slides/pack)
HuCAT116	TissueArray	5 slides	EUR 150

Human Colon Cancer (single section per slide) (5 slides/pack)
HuCAT121	TissueArray	5 slides	EUR 150

Human Colon Cancer (single section per slide) (5 slides/pack)
HuCAT126	TissueArray	5 slides	EUR 150

Human Colon Cancer (single section per slide) (5 slides/pack)
HuCAT131	TissueArray	5 slides	EUR 150

Human Colon Cancer (single section per slide) (5 slides/pack)
HuCAT136	TissueArray	5 slides	EUR 150

Human Rectum Cancer (single section per slide) (5 slides/pack)
HuCAT141	TissueArray	5 slides	EUR 150

Human Rectum Cancer (single section per slide) (5 slides/pack)
HuCAT146	TissueArray	5 slides	EUR 150

Human Rectum Cancer (single section per slide) (5 slides/pack)
HuCAT151	TissueArray	5 slides	EUR 150

Human Rectum Cancer (single section per slide) (5 slides/pack)
HuCAT156	TissueArray	5 slides	EUR 150

Human Rectum Cancer (single section per slide) (5 slides/pack)
HuCAT161	TissueArray	5 slides	EUR 150

Human Pancreatic Cancer (single section per slide) (5 slides/pack)
HuCAT166	TissueArray	5 slides	EUR 150

Human Pancreatic Cancer (single section per slide) (5 slides/pack)
HuCAT167	TissueArray	5 slides	EUR 150

Human Pancreatic Cancer (single section per slide) (5 slides/pack)
HuCAT171	TissueArray	5 slides	EUR 150

Human Pancreatic Cancer (single section per slide) (5 slides/pack)
HuCAT176	TissueArray	5 slides	EUR 150

Human Lung Cancer (single section per slide) (5 slides/pack)
HuCAT196	TissueArray	5 slides	EUR 150

Human Lung Cancer (single section per slide) (5 slides/pack)
HuCAT200	TissueArray	5 slides	EUR 150

Human Lung Cancer (single section per slide) (5 slides/pack)
HuCAT206	TissueArray	5 slides	EUR 150

Human Lung Cancer (single section per slide) (5 slides/pack)
HuCAT211	TissueArray	5 slides	EUR 150

Human Lung Cancer (single section per slide) (5 slides/pack)
HuCAT216	TissueArray	5 slides	EUR 150

Human Lung Cancer (single section per slide) (5 slides/pack)
HuCAT221	TissueArray	5 slides	EUR 150

Human Lung Cancer (single section per slide) (5 slides/pack)
HuCAT226	TissueArray	5 slides	EUR 150

Human Lung Cancer (single section per slide) (5 slides/pack)
HuCAT231	TissueArray	5 slides	EUR 150

Human Lung Adenocarcinoma (single section per slide) (5 slides/pack)
HuCAT232	TissueArray	5 slides	EUR 150

Human Endometrium Cancer (single section per slide) (5 slides/pack)
HuCAT266	TissueArray	5 slides	EUR 150

Human Endometrium Cancer (single section per slide) (5 slides/pack)
HuCAT271	TissueArray	5 slides	EUR 150

Human Endometrium Cancer (single section per slide) (5 slides/pack)
HuCAT276	TissueArray	5 slides	EUR 150

Human Endometrium Cancer (single section per slide) (5 slides/pack)
HuCAT281	TissueArray	5 slides	EUR 150

Human Endometrium Cancer (single section per slide) (5 slides/pack)
HuCAT286	TissueArray	5 slides	EUR 150

Human Breast Cancer (single section per slide) (5 slides/pack)
HuCAT291	TissueArray	5 slides	EUR 150

Human Breast Cancer (single section per slide) (5 slides/pack)
HuCAT296	TissueArray	5 slides	EUR 150

Human Breast Cancer (single section per slide) (5 slides/pack)
HuCAT297	TissueArray	5 slides	EUR 150

Human Breast Cancer (single section per slide) (5 slides/pack)
HuCAT298	TissueArray	5 slides	EUR 150

Human Breast Cancer (single section per slide) (5 slides/pack)
HuCAT299	TissueArray	5 slides	EUR 150

Human Breast Cancer (single section per slide) (5 slides/pack)
HuCAT300	TissueArray	5 slides	EUR 150

Human Breast Cancer (single section per slide) (5 slides/pack)
HuCAT301	TissueArray	5 slides	EUR 150

Human Breast Cancer (single section per slide) (5 slides/pack)
HuCAT303	TissueArray	5 slides	EUR 150

Human Ovary Cancer (single section per slide) (5 slides/pack)
HuCAT306	TissueArray	5 slides	EUR 150

Human Ovary Cancer (single section per slide) (5 slides/pack)
HuCAT311	TissueArray	5 slides	EUR 150

Human Ovary Cancer (single section per slide) (5 slides/pack)
HuCAT316	TissueArray	5 slides	EUR 150

Human Ovary Cancer (single section per slide) (5 slides/pack)
HuCAT321	TissueArray	5 slides	EUR 150

Human Ovary Cancer (single section per slide) (5 slides/pack)
HuCAT326	TissueArray	5 slides	EUR 150

Human Ovary Cancer (single section per slide) (5 slides/pack)
HuCAT331	TissueArray	5 slides	EUR 150

Human Ovary Cancer (single section per slide) (5 slides/pack)
HuCAT336	TissueArray	5 slides	EUR 150

Human Ovary Cancer (single section per slide) (5 slides/pack)
HuCAT341	TissueArray	5 slides	EUR 150

Human Spleen Cancer (single section per slide) (5 slides/pack)
HuCAT346	TissueArray	5 slides	EUR 150

Human Spleen Cancer (single section per slide) (5 slides/pack)
HuCAT351	TissueArray	5 slides	EUR 150

Human Spleen Cancer (single section per slide) (5 slides/pack)
HuCAT356	TissueArray	5 slides	EUR 150

Human Prostate Cancer (single section per slide) (5 slides/pack)
HuCAT361	TissueArray	5 slides	EUR 150

Human Prostate Cancer (single section per slide) (5 slides/pack)
HuCAT366	TissueArray	5 slides	EUR 150

Human Prostate Cancer (single section per slide) (5 slides/pack)
HuCAT367	TissueArray	5 slides	EUR 150

Human Prostate Cancer (single section per slide) (5 slides/pack)
HuCAT371	TissueArray	5 slides	EUR 150

Human Prostate Cancer (single section per slide) (5 slides/pack)
HuCAT376	TissueArray	5 slides	EUR 150

Human Testis Cancer (single section per slide) (5 slides/pack)
HuCAT381	TissueArray	5 slides	EUR 150

Human Testis Cancer (single section per slide) (5 slides/pack)
HuCAT386	TissueArray	5 slides	EUR 150

Human Kidney Cancer (single section per slide) (5 slides/pack)
HuCAT391	TissueArray	5 slides	EUR 150

Human Kidney Cancer (single section per slide) (5 slides/pack)
HuCAT396	TissueArray	5 slides	EUR 150

Human Kidney Cancer (single section per slide) (5 slides/pack)
HuCAT401	TissueArray	5 slides	EUR 150

Human Kidney Cancer (single section per slide) (5 slides/pack)
HuCAT406	TissueArray	5 slides	EUR 150

Human Kidney Cancer (single section per slide) (5 slides/pack)
HuCAT411	TissueArray	5 slides	EUR 150

Human Bladder Cancer (single section per slide) (5 slides/pack)
HuCAT416	TissueArray	5 slides	EUR 150

Human Bladder Cancer (single section per slide) (5 slides/pack)
HuCAT417	TissueArray	5 slides	EUR 150

Human Bladder Cancer (single section per slide) (5 slides/pack)
HuCAT418	TissueArray	5 slides	EUR 150

Human Bladder Cancer (single section per slide) (5 slides/pack)
HuCAT421	TissueArray	5 slides	EUR 150

Human Bladder Cancer (single section per slide) (5 slides/pack)
HuCAT426	TissueArray	5 slides	EUR 150

Human Bone Cancer (single section per slide) (5 slides/pack)
HuCAT446	TissueArray	5 slides	EUR 150

Human Bone Cancer (single section per slide) (5 slides/pack)
HuCAT451	TissueArray	5 slides	EUR 150

Human Bone Cancer (single section per slide) (5 slides/pack)
HuCAT456	TissueArray	5 slides	EUR 150

Human Cartilage Cancer (single section per slide) (5 slides/pack)
HuCAT461	TissueArray	5 slides	EUR 150

Human Cartilage Cancer (single section per slide) (5 slides/pack)
HuCAT466	TissueArray	5 slides	EUR 150

Human Tongue Cancer (single section per slide) (5 slides/pack)
HuCAT471	TissueArray	5 slides	EUR 150

Human Tongue Cancer (single section per slide) (5 slides/pack)
HuCAT476	TissueArray	5 slides	EUR 150

It is generally assumed that the microscope slides are lost during the bombing of the Zoological Institute in Würzburg at the end of World War II. Here, I describe the discovery of a number of original microscope slides Boveri with early serial section and Ascaris sea urchin embryo, stained with hematoxylin iron HEIDENHAIN this method. Some slides bear handwritten notes and sketches by Boveri. Evidence is presented that the new slide is found is part of the original materials used by Boveri’s seminal monograph centrosome.