BACKGROUND
The purpose of this study was to compare the digital image of the biopsy tissue taken with an optical microscope using bright field techniques under various lighting conditions. Various colors in immunohistochemistry stained with 3,3′-diaminobenzidine and Haematoxylin very large and tissue samples derived from various sources. One of them is the inadequate arrangements camera’s white balance color temperature of the light microscope.

Although these types of errors can be easily handled during the image acquisition phase, can be eliminated by using a color adjustment algorithms. Examination of the color variation of the temperature dependence of light microscope and camera settings is done as a preliminary study for automatic color standardization process.

METHOD
Six field of view with an empty space between these tissue samples selected for analysis. Each field of view has acquired 225 times with mild temperatures variety of microscopes and camera white balance settings. Fourteen of randomly selected images has been improved and compared with reference images, by the following methods: Mean Square Error, Structural similarity and a visual assessment viewer.

RESULTS
For the two types of background and two types of objects, descriptors statistical picture: the range, median, mean and standard deviation of the chromaticity on the A and B channels of the CIELAB color space, and lighting L, and the variability of local color to a specific area objects have counted. The result has been the average over the six images obtained in the same light conditions and camera settings for each sample.

CONCLUSION
Analysis of lead results following conclusions: (1) the images collected with the white balance adjusted for cluster color temperature of light in certain regions of space chromatic, (2) the process of correction of white balance to the images collected by the camera white balance settings are not suitable for the temperature light the explanatory moving picture to the right color space but at the same time value the lighting changes. So the unification process in terms of color fidelity image can be completed in a separate introductory stage before automatic image analysis.

centrosome research historical roots: the invention of the microscope slide Boveri in Würzburg.

Boveri visionary monograph ‘Ueber die Natur der Centrosomen’ (On the nature of centrosomes) in 1900 founded largely on microscopic observations of sea urchin egg divides and Ascaris roundworm parasites.

As Boveri wrote in the introductory paragraph, less importance on morphological aspects of centrosomes, but aimed at an understanding of their physiological roles during cell division. The remarkable transition from observations of small dot-like structure remains and pieces of material for a unified theory of centrosome function (which is basically still true today) can not be fully appreciated without checking this starting material Boveri, histological specimens.

Microscope Slides, Plain
07441-1	Polysciences Europe GmbH	1box	EUR 50

Microscope Slides, Micropure
22245-1	Polysciences Europe GmbH	1box	EUR 32

Microscope Slides, Micropure
22245-20	Polysciences Europe GmbH	1box	EUR 380

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DD52951	Scientific Laboratory Supplies	PK144	EUR 24.62

Microscope Slides, Immunofluorescence
18357-1	Polysciences Europe GmbH	1box	EUR 168

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26414-1	Polysciences Europe GmbH	1box	EUR 82

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26414-20	Polysciences Europe GmbH	20box	EUR 585

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PO102	FD Neurotechnologies	100 slides	EUR 236.4

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PO202	FD Neurotechnologies	each	EUR 73.2

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Description: Glass Slides, ?Yellow Color Frosted?One

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Description: Glass Slides, ?Yellow Color Frosted?One

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"Glass Slides, ?Orange Color Frosted?One
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Description: Glass Slides, ?Blue Color Frosted?One E

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M7100-SD	MTC Bio	1/pack	EUR 38.23
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AutoFrost Enhanced IHC Microscope Slides, Clipped corner, White, 3000pcs/case
EMP00W3	IHC World	-	EUR 1250

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M-ACC-GSL9	MiTeGen	200 TIPS	EUR 65
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24764-1	Polysciences Europe GmbH	1each	EUR 148

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19801A-1	Polysciences Europe GmbH	1Box	EUR 56

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LC7011-100	GenDepot	100/CS	EUR 684

Microscope calibration slide #1
LBI10411	Westburg	each	EUR 1155.4

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Microscope Slide Boxes,100-Place, Yellow
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Microscope Slide Boxes,25-Place, Yellow
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Microscope Slide Boxes,25-Place, Yellow
LC7010-010	GenDepot	10/PK	EUR 148.8

Microscope Slide Boxes,25-Place, Yellow
LC7010-100	GenDepot	100/CS	EUR 684

Microscope Slide Storage Cabinet Base Unit
22353-1	Polysciences Europe GmbH	1each	EUR 397

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BG004-10X50NO	EWC Diagnostics	1 unit	EUR 24.77
Description: Glass Slides, Cut Edges

Glass Slides, Cut Edges
BG004-5X50NO	EWC Diagnostics	1 unit	EUR 13.53
Description: Glass Slides, Cut Edges

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16603-1	Polysciences Europe GmbH	1bag	EUR 28

Microscope Slide Storage Box, Plastic large box
16604-1	Polysciences Europe GmbH	1each	EUR 54

Microscope Slide Coverslips, Glass, 22mm x 22mm
21913-1	Polysciences Europe GmbH	1oz	EUR 40

Microscope Slide Coverslips, Glass, 22mm x 30mm
23999-1	Polysciences Europe GmbH	1oz	EUR 40

Microscope Slide Coverslips, Glass, 24mm x 40mm
24070-1	Polysciences Europe GmbH	1oz	EUR 40

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24071-1	Polysciences Europe GmbH	1oz	EUR 41

Microscope Slide Coverslips, Glass, 24mm x 60mm
24072-1	Polysciences Europe GmbH	1oz	EUR 53

2 Place Microscope Slide Mailer PP Natural - PK25
MIC3172	Scientific Laboratory Supplies	PK25	EUR 52.79

Glass Slides, ?Ground Edges, Plain,?Fro
BG003-10X50NO	EWC Diagnostics	1 unit	EUR 36.11
Description: Glass Slides, ?Ground Edges, Plain,?Fro

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BG003-5X50NO	EWC Diagnostics	1 unit	EUR 19.5
Description: Glass Slides, Ground Edges, Plain, Fro

Quartz Microscope Slide, 1"x2" (25.4 x 50.8 mm) x 0.5 mm Thin
24962-1	Polysciences Europe GmbH	1EA	EUR 217

Quartz Microscope Slide, 1"x3" (25.4 x 76.2 mm) x 0.5 mm Thin
24963-1	Polysciences Europe GmbH	1EA	EUR 192

Premium Microscope Slide Box 50-Place Cork Red - EACH
MIC4216	Scientific Laboratory Supplies	EACH	EUR 24.14

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24957-1	Polysciences Europe GmbH	1EA	EUR 58

Premium Microscope Slide Box 50-Place Cork Blue - EACH
MIC4214	Scientific Laboratory Supplies	EACH	EUR 29.84

2 Place Microscope Slide Mailer PP Assorted Colours - PK25
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BG001-10X50NO	EWC Diagnostics	1 unit	EUR 27.61
Description: Glass Slides, ?Ground Edges, Plain

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Description: Glass Slides, Ground Edges, Plain

Glass Slides, ?Ground Edges, Plain
BG002-10X50NO	EWC Diagnostics	1 unit	EUR 27.61
Description: Glass Slides, ?Ground Edges, Plain

Glass Slides, ?Ground Edges, Plain
BG002-5X50NO	EWC Diagnostics	1 unit	EUR 14.96
Description: Glass Slides, ?Ground Edges, Plain

FlyStuff Microscope
59-502	Genesee Scientific	1 Microscope/Unit	EUR 1801.67
Description: Binocular, w/base

SLS Slides Single Cavity - PK50
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Description: Microscope Cover Glass

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BG011C-5X100NO	EWC Diagnostics	1 unit	EUR 23.46
Description: Microscope Cover Glass

Microscope Cover Glass
BG012C-5X100NO	EWC Diagnostics	1 unit	EUR 31.97
Description: Microscope Cover Glass

Microscope Cover Glass
BG013C-10X100NO	EWC Diagnostics	1 unit	EUR 65.86
Description: Microscope Cover Glass

Microscope Cover Glass
BG013C-5X100NO	EWC Diagnostics	1 unit	EUR 34.89
Description: Microscope Cover Glass

Microscope Cover Glass
BG014C-10X100NO	EWC Diagnostics	1 unit	EUR 106.84
Description: Microscope Cover Glass

Microscope Cover Glass
BG014C-5X100NO	EWC Diagnostics	1 unit	EUR 56.25
Description: Microscope Cover Glass

Microscope Cover Glass
BG015C-10X100NO	EWC Diagnostics	1 unit	EUR 72.85
Description: Microscope Cover Glass

Microscope Cover Glass
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Description: Microscope Cover Glass

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59-503	Genesee Scientific	1 Microscope/Unit	EUR 2779.21
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25039-1	Polysciences Europe GmbH	1EA	EUR 19098

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25040-1	Polysciences Europe GmbH	1EA	EUR 15134

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MIC1590	Scientific Laboratory Supplies	EACH	EUR 1433.55

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MIC2800	Scientific Laboratory Supplies	EACH	EUR 1357.74

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MIC1968	Scientific Laboratory Supplies	EACH	EUR 1827.42

ReadiUseâ„¢ microscope mounting solution
20009	AAT Bioquest	50 mL	EUR 88
Description: The mounting medium is the solution in which the specimen is embedded, generally under a cover glass.

ReadiUseâ„¢ microscope mounting solution
20009-50mL	AAT Bioquest	50 mL	EUR 86
Description: The mounting medium is the solution in which the specimen is embedded, generally under a cover glass.

Polyp (5 slides/pack, single section/slide)
S-HuDAT177	TissueArray	1 unit	EUR 95

Microscope Cover Glass (Borosilicate G
BG012C-10X100NO	EWC Diagnostics	1 unit	EUR 60.71
Description: Microscope Cover Glass (Borosilicate G

BA310 Elite Trinocular microscope - EACH
MIC2937	Scientific Laboratory Supplies	EACH	EUR 3000.76

BA210E Elite Binocular Microscope - EACH
MIC5633	Scientific Laboratory Supplies	EACH	EUR 2035.76

Adenosis (5 slides/pack, single section/slide)
S-HuDAT085	TissueArray	1 unit	EUR 95

Nephritis (5 slides/pack, single section/slide)
S-HuDAT016	TissueArray	1 unit	EUR 95

Microscope Pack 2 CX23 BIN LED - EACH
MIC1970	Scientific Laboratory Supplies	EACH	EUR 1865.38

Fibroadenoma (5 slides/pack, single section/slide)
S-HuDAT048	TissueArray	1 unit	EUR 95

Digi-Pad Microscope Tablet Camera - EACH
MIC0115	Scientific Laboratory Supplies	EACH	EUR 2033.1

Human Lung (5 slides/pack, single section/slide)
S-HuCAT1280	TissueArray	1 unit	EUR 115

Human Lung (5 slides/pack, single section/slide)
S-HuCAT1283	TissueArray	1 unit	EUR 115

Human Lung (5 slides/pack, single section/slide)
S-HuCAT1287	TissueArray	1 unit	EUR 115

Human Lung (5 slides/pack, single section/slide)
S-HuCAT1288	TissueArray	1 unit	EUR 115

Human Lung (5 slides/pack, single section/slide)
S-HuCAT1296	TissueArray	1 unit	EUR 115

Human Lung (5 slides/pack, single section/slide)
S-HuCAT1303	TissueArray	1 unit	EUR 115

Human Lung (5 slides/pack, single section/slide)
S-HuCAT1307	TissueArray	1 unit	EUR 115

Human Lung (5 slides/pack, single section/slide)
S-HuCAT1309	TissueArray	1 unit	EUR 115

Human Lung (5 slides/pack, single section/slide)
S-HuCAT1311	TissueArray	1 unit	EUR 115

Human Lung (5 slides/pack, single section/slide)
S-HuCAT1312	TissueArray	1 unit	EUR 115

Human Skin (5 slides/pack, single section/slide)
S-HuCAT1321	TissueArray	1 unit	EUR 115

Human Skin (5 slides/pack, single section/slide)
S-HuCAT1322	TissueArray	1 unit	EUR 115

Human Lung (5 slides/pack, single section/slide)
S-HuCAT208	TissueArray	1 unit	EUR 115

Human Lung (5 slides/pack, single section/slide)
S-HuCAT210	TissueArray	1 unit	EUR 115

It is generally assumed that the microscope slides are lost during the bombing of the Zoological Institute in Würzburg at the end of World War II. Here, I describe the discovery of a number of original microscope slides Boveri with early serial section and Ascaris sea urchin embryo, stained with hematoxylin iron HEIDENHAIN this method. Some slides bear handwritten notes and sketches by Boveri. Evidence is presented that the new slide is found is part of the original materials used by Boveri’s seminal monograph centrosome.