The influence of the microscope lamp filament colour temperature on the process of digital images of histological slides acquisition standardization.

The influence of the microscope lamp filament colour temperature on the process of digital images of histological slides acquisition standardization.

BACKGROUND
The purpose of this study was to compare the digital image of the biopsy tissue taken with an optical microscope using bright field techniques under various lighting conditions. Various colors in immunohistochemistry stained with 3,3′-diaminobenzidine and Haematoxylin very large and tissue samples derived from various sources. One of them is the inadequate arrangements camera’s white balance color temperature of the light microscope.

Although these types of errors can be easily handled during the image acquisition phase, can be eliminated by using a color adjustment algorithms. Examination of the color variation of the temperature dependence of light microscope and camera settings is done as a preliminary study for automatic color standardization process.


METHOD
Six field of view with an empty space between these tissue samples selected for analysis. Each field of view has acquired 225 times with mild temperatures variety of microscopes and camera white balance settings. Fourteen of randomly selected images has been improved and compared with reference images, by the following methods: Mean Square Error, Structural similarity and a visual assessment viewer.


RESULTS
For the two types of background and two types of objects, descriptors statistical picture: the range, median, mean and standard deviation of the chromaticity on the A and B channels of the CIELAB color space, and lighting L, and the variability of local color to a specific area objects have counted. The result has been the average over the six images obtained in the same light conditions and camera settings for each sample.


CONCLUSION
Analysis of lead results following conclusions: (1) the images collected with the white balance adjusted for cluster color temperature of light in certain regions of space chromatic, (2) the process of correction of white balance to the images collected by the camera white balance settings are not suitable for the temperature light the explanatory moving picture to the right color space but at the same time value the lighting changes. So the unification process in terms of color fidelity image can be completed in a separate introductory stage before automatic image analysis.

 The influence of the microscope lamp filament colour temperature on the process of digital images of histological slides acquisition standardization.
The influence of the microscope lamp filament colour temperature on the process of digital images of histological slides acquisition standardization.

centrosome research historical roots: the invention of the microscope slide Boveri in Würzburg.

Boveri visionary monograph ‘Ueber die Natur der Centrosomen’ (On the nature of centrosomes) in 1900 founded largely on microscopic observations of sea urchin egg divides and Ascaris roundworm parasites.

As Boveri wrote in the introductory paragraph, less importance on morphological aspects of centrosomes, but aimed at an understanding of their physiological roles during cell division. The remarkable transition from observations of small dot-like structure remains and pieces of material for a unified theory of centrosome function (which is basically still true today) can not be fully appreciated without checking this starting material Boveri, histological specimens.

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It is generally assumed that the microscope slides are lost during the bombing of the Zoological Institute in Würzburg at the end of World War II. Here, I describe the discovery of a number of original microscope slides Boveri with early serial section and Ascaris sea urchin embryo, stained with hematoxylin iron HEIDENHAIN this method. Some slides bear handwritten notes and sketches by Boveri. Evidence is presented that the new slide is found is part of the original materials used by Boveri’s seminal monograph centrosome.

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